Molecular phytogeography of Aster and Kalimeris in East Asia

东亚紫菀和马兰的分子植物地理学

• 批准号: 07041126
• 负责人: ITO Motomi
• 金额: $ 1.66万
• 依托单位: CHIBA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07041126/
• 关键词: Aster; Kalimeris; molecular systematics; polyploid complex; speciation

Morphological, cytological and molecular analysis of Aster ageratoides complex in Taiwan had been studied. We collected about 200 plants from 15 population in Taiwan. In morphological study, four taxa were recognized, the two wide spread species and the two species restricted in limestone area. Among the wide spread species, one type is almost glabrous and identified as A.ageratoides, and the other type is pubescent and identified as A.lasoclada.From the cytological analysis, all plants belonging Aster ageratoides were tetraploid and A.lasioclada were diploid. They occurs allopatrically each other. We had performed the RFLP analysis of cpDNA of the plants collected in Taiwan. As a result, these two species showed difference haplotypes. For comparing to the plants in Japan and Korea, we had reconstructed the phylogenetic tree of the result of present study and previous result of Japanese and Korean plants. The most parsimonious tree showed a topology which A.ageratoides of Taiwan clustered together with Japanese and Korean A.ageratoides and A.lasioclada branched at base of the tree. This result suggests that there are two lineages in Aster ageratoides complex in Taiwan.

对台湾三脉紫菀复合体进行形态学、细胞学及分子生物学研究。本研究采自台湾15个居群约200株植物。在形态学研究中，共鉴定出4个类群，其中广布种2个，局限种2个。在分布广泛的种中，有一种几乎无毛，鉴定为三脉紫菀（A.ageratoides），另一种被短柔毛，鉴定为毛枝紫菀（A.lasoclada），细胞学分析表明，三脉紫菀（A.ageratoides）为四倍体，毛枝紫菀（A.lasioclada）为二倍体。它们彼此异地发生。本研究对采自台湾的植物进行了cpDNA的限制性片段长度多态性分析。结果表明，这两个物种表现出不同的单倍型。为了与日本和韩国的植物进行比较，我们将本研究的结果与日本和韩国的已有结果进行了系统发育树的重建。最简约的树状图显示台湾的三尖蒿与日本、韩国的三尖蒿聚在一起，毛枝蒿在树的基部分枝。此结果显示台湾三脉紫菀复合体有两个支系。

项目成果

M.Ito,A.Soejima,M.Hasebe and K.Watanabe: "A chloroplast-DNA phylogeny of Kalimeris and Aster,with reference to the generic circumscription" Journal of Plant Research. 108. 93-96 (1995)

M.Ito、A.Soejima、M.Hasebe 和 K.Watanabe：“Kalimeris 和 Aster 的叶绿体 DNA 系统发育，参考通用范围”植物研究杂志。

A.Soejima and C.I.Peng: "Aster ageratoides complex in Taiwan. Cytological and morphological features." Bot. Bull. Acad. Sinica. 23 (in press). (1996)

A.Soejima 和 C.I.Peng：“台湾紫菀复合体。细胞学和形态特征。”