Dynamism of apoplast

质外体的动力学

基本信息

  • 批准号:
    07304054
  • 负责人:
  • 金额:
    $ 9.73万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
  • 财政年份:
    1995
  • 资助国家:
    日本
  • 起止时间:
    1995 至 1996
  • 项目状态:
    已结题

项目摘要

The following results were obtained ;(1) Five glucanases bound to barley cell walls were identified as, [1] endo-1,3 ; 1,4-glucanase, [2] endo-1,3-glucanase-1, [3] endo-1,3-glucanase-2, [4] exoglucanase-1, and [5] exoglucanase-2. The enzymes involved in 1,3 ; 1,4-glucan degradation in the cereal plants are [1] and [4].(2) The enzymic activity that converts indoleacetaldehyde to indole-3-acetic acid was found in barley cell walls. The Km was 5muM which is lower than that for the cytoplasmic enzyme with similar activity (31muM). Apoplast of dark grown squash hypocotyls containes the concentration of indole-3-acetic acid 5 times higher than the symplast.(3) Essential element, boron, which was known to be localized at cell walls, was bound to rhamnogalacturoans by a diol-ester linkage and cross-linked two polysaccharides.(4) It was suggested that when xyloglucan was breakdown during elongation growth, xyloglucans becomes susceptible to enzyme action after cellulose molecules into which zyloglucan was inserted, was degraded by endoglucanase activity.(5) In is unlikely that plant has RGD peptide homologs similar to those found on animal cells.The technique with pH dependent fluorescence dye revealed that the phosphate lever of vacuoles were homeostatically regulted by the cooperative action of mechanism wiht which the cytoplasmic phosphate level was regulated.These results clearly demonstrate that the apoplast is the site where substances prerequisite for the plant life, such as plant hormones, polysaccharide-related enzymes, or inorganic ions, are dynamically metabolized.
结果表明:(1)5种与大麦细胞壁结合的葡聚糖酶被鉴定为:[1]内切-1,3; 1,4-葡聚糖酶,[2]内切-1,3-葡聚糖酶-1,[3]内切-1,3-葡聚糖酶-2,[4]外切葡聚糖酶-1和[5]外切葡聚糖酶-2。禾谷类植物中参与1,3; 1,4-葡聚糖降解的酶为[1]和[4]。(2)在大麦细胞壁中发现了将吲哚乙醛转化为吲哚-3-乙酸的酶活性。Km值为5 μ M,低于活性相近的胞质酶Km值(31 μ M)。暗培养西葫芦下胚轴质外体中吲哚-3-乙酸的浓度比共质体高5倍。(3)必需元素硼,已知定位于细胞壁,通过二醇-酯键和交联的两种多糖与鼠李糖半乳果糖结合。(4)有人建议,当木葡聚糖在伸长生长过程中被分解时,木葡聚糖在插入了木葡聚糖的纤维素分子被内切葡聚糖酶活性降解后变得对酶作用敏感。(5)植物中不可能存在与动物细胞中类似的RGD肽同源物。pH依赖性荧光染料技术表明,液泡中的磷酸盐水平是由细胞质磷酸盐水平调节机制的协同作用来调节的。这些结果清楚地表明,质外体是植物生命所必需的物质,如植物激素,多糖相关酶或无机离子被动态代谢。

项目成果

期刊论文数量(115)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
M. Kobayashi, T. Matoh and J. Azuma: "Two Chains of Rhamnogalacturonan II are Cross linked by Borate-Diol EsterBonds in Higher Plant Cell Walls" Plant Physiology. 110(in press). (1996)
M. Kobayashi、T. Matoh 和 J. Azuma:“在高等植物细胞壁中,鼠李糖半乳糖醛酸 II 的两条链通过硼酸酯-二醇酯键交联”《植物生理学》。
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    0
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K. Nishitani: "The role of-endoxyloglucan transferase in the organization of plant cell walls" International Review of Cytology. 173. 157-206 (1997)
K. Nishitani:“内切木葡聚糖转移酶在植物细胞壁组织中的作用”《国际细胞学评论》。
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    0
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K.Nishitani: "The role of endoxyloglucun transferase in the organization of plant cell walls" International Review of Cytology. 173. 157-206 (1997)
K.Nishitani:“内切酶在植物细胞壁组织中的作用”国际细胞学评论。
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    0
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Wakabayashi, K., T.Hoson and S.Kamisaka: "Osmotic stress suppresses cell wall stiffening and the increase in cell wall-gound ferulic and diferulic acids in wheat coleoptiles." Plant Physiol.113(in press). (1997)
Wakabayashi, K.、T.Hoson 和 S.Kamisaka:“渗透压会抑制小麦胚芽鞘中细胞壁的硬化和细胞壁阿魏酸和二阿魏酸的增加。”
  • DOI:
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  • 影响因子:
    0
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Kotake, T., N.Nakagawa, K.Takeda and N.Sakurai: "Purification and characterization of wall-bound exo-1,3, -beta-glucanase from barley (Hordeum vulgare L.) seedlings." Plant Cell Physiol.38. 194-200 (1997)
Kotake, T.、N.Nakakawa、K.Takeda 和 N.Sakurai:“来自大麦 (Hordeum vulgare L.) 幼苗的壁结合外切 1,3, -β-葡聚糖酶的纯化和表征。”
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    0
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SAKURAI Naoki其他文献

SAKURAI Naoki的其他文献

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{{ truncateString('SAKURAI Naoki', 18)}}的其他基金

A research on the actual state and effectiveness of the fiscal transfer system in educational budgeting of local governments
地方政府教育预算中财政转移支付制度的现状及有效性研究
  • 批准号:
    16H07392
  • 财政年份:
    2016
  • 资助金额:
    $ 9.73万
  • 项目类别:
    Grant-in-Aid for Research Activity Start-up
The Development of the swallowing function evaluation training system for home care patients applied ICT (Information and Communication Technology) and piezo sensor
应用ICT(信息通信技术)和压电传感器开发家庭护理患者吞咽功能评估训练系统
  • 批准号:
    25462990
  • 财政年份:
    2013
  • 资助金额:
    $ 9.73万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of the remote swallowing function evaluation training system with a piezo sensor for the home-bound elderly patient
居家老年患者压电传感器远程吞咽功能评估训练系统的开发
  • 批准号:
    22592145
  • 财政年份:
    2010
  • 资助金额:
    $ 9.73万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of non-destructive method for water content and internal defect of trees with an acoustic vibration technique
利用声振动技术开发树木含水量和内部缺陷的无损检测方法
  • 批准号:
    19380021
  • 财政年份:
    2007
  • 资助金额:
    $ 9.73万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of diagnosis support system for mastication-swallowing function
咀嚼吞咽功能诊断支持系统的开发
  • 批准号:
    19592231
  • 财政年份:
    2007
  • 资助金额:
    $ 9.73万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of measuring system of maturity and quality of fruits by laser Doppler method
激光多普勒法水果成熟度及品质测定系统的研制
  • 批准号:
    12556006
  • 财政年份:
    2000
  • 资助金额:
    $ 9.73万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
A follow-up study on the relation of malocclusion and temporomandibular joint MRI and helical CT findings in temporomandibular disorder patients
颞下颌关节紊乱病患者咬合不正与颞下颌关节MRI及螺旋CT表现关系的随访研究
  • 批准号:
    11671926
  • 财政年份:
    1999
  • 资助金额:
    $ 9.73万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Studies on the mechanism of auxin-induced elongation growth in Poaceae plants
生长素诱导禾本科植物伸长生长机制的研究
  • 批准号:
    07640864
  • 财政年份:
    1995
  • 资助金额:
    $ 9.73万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Production of a remote measuring system of fruit softening using laser beams and an acoustic wave
使用激光束和声波生产水果软化远程测量系统
  • 批准号:
    07556012
  • 财政年份:
    1995
  • 资助金额:
    $ 9.73万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
ANALYSIS OF DWARF GENE(UZ)OF STRAIN UZU-AKASHINNRIKI OF BARLEY
大麦UZU-AKASHINRIKI矮化基因(UZ)分析
  • 批准号:
    63540534
  • 财政年份:
    1988
  • 资助金额:
    $ 9.73万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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微生物的可溶性酶如何降解不溶性植物细胞壁?
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旨在通过增加纤维素对压缩刺激的反应来发现细胞壁的新功能
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