Dynamism of apoplast

质外体的动力学

基本信息

批准号：
07304054
负责人：
SAKURAI Naoki
金额：
$ 9.73万
依托单位：
Hiroshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

The following results were obtained ;(1) Five glucanases bound to barley cell walls were identified as, [1] endo-1,3 ; 1,4-glucanase, [2] endo-1,3-glucanase-1, [3] endo-1,3-glucanase-2, [4] exoglucanase-1, and [5] exoglucanase-2. The enzymes involved in 1,3 ; 1,4-glucan degradation in the cereal plants are [1] and [4].(2) The enzymic activity that converts indoleacetaldehyde to indole-3-acetic acid was found in barley cell walls. The Km was 5muM which is lower than that for the cytoplasmic enzyme with similar activity (31muM). Apoplast of dark grown squash hypocotyls containes the concentration of indole-3-acetic acid 5 times higher than the symplast.(3) Essential element, boron, which was known to be localized at cell walls, was bound to rhamnogalacturoans by a diol-ester linkage and cross-linked two polysaccharides.(4) It was suggested that when xyloglucan was breakdown during elongation growth, xyloglucans becomes susceptible to enzyme action after cellulose molecules into which zyloglucan was inserted, was degraded by endoglucanase activity.(5) In is unlikely that plant has RGD peptide homologs similar to those found on animal cells.The technique with pH dependent fluorescence dye revealed that the phosphate lever of vacuoles were homeostatically regulted by the cooperative action of mechanism wiht which the cytoplasmic phosphate level was regulated.These results clearly demonstrate that the apoplast is the site where substances prerequisite for the plant life, such as plant hormones, polysaccharide-related enzymes, or inorganic ions, are dynamically metabolized.

获得了以下结果；（1）将与大麦细胞壁结合的五个葡萄糖酶确定为[1] Endo-1,3； 1,4-葡萄糖酶，[2]内多-1,3-葡聚糖酶-1，[3] endo-1,3-葡萄糖酶-2，[4] exogoganase-1和[5] exogoglucanase-2。涉及1,3的酶；谷物植物中的1,4-葡聚糖降解是[1]和[4]。（2）在大麦细胞壁中发现了将吲哚羟醛转化为吲哚-3-乙酸的酶活性。 Km为5MUM，比具有相似活性的细胞质酶（31MUM）低。深色种植壁球的低尾胞素的凋亡含有吲哚 - 3-乙酸的浓度5倍。（3）基本要素，已知硼龙（已知位于细胞壁上）与rhamnogalcalacturoans绑定，rhamnogalacturolans被二醇 - 层连接和交叉连接的两种多细多糖液在xylog中均为xylog。木糖葡萄糖在插入二凝蛋白葡萄糖的纤维素分子后易于酶作用，被内og葡萄酶的活性降解。（5）在植物中，植物不太可能具有与动物细胞相似的RGD肽同源物，该技术通过pH依赖性荧光染色体，该技术呈现了pH依赖性的作用。细胞质磷酸盐水平受到调节的机制。这些结果清楚地表明，凋亡是植物寿命的物质先决条件的位置，例如植物激素，多糖相关酶或无机离子，或者是动力代谢的。