Studies on the mechanism of auxin-induced elongation growth in Poaceae plants

生长素诱导禾本科植物伸长生长机制的研究

• 批准号: 07640864
• 负责人: SAKURAI Naoki
• 金额: $ 1.41万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07640864/
• 关键词: beta-glucanase; cell walls; auxin; elongation growth; enzyme purification; proteins; N terminal amino acids; barley; 伸長生長

We purified and identified five glucanases bound to barley cell walls ; [1], endo-1,3 ; 1,4-glucanase (31KD) ; [2], endo-1,3-glucanase-1 (31kD) ; [3], endo-1,3-glucanase-2 (31kD) ; [4], exo-glucanase-1 (66kD) ; [5], exo-glucanase-2 (68kD). The enzymes involved in 1,3 ; 1,4-glucan degradation in cereal plants are likely glucanase [1] and [4]. The exo-glucanase-1 [4] has the highest activity toward 1,3 ; 1,4-beta-glucans, and accounts for the 60% of total glucanase activity bound to the cell walls. The endo-glucanase [1] caused a drastic molecular downshift of 1,3 ; 1,4-beta-glucans. N-terminal amino acid sequences of glucanase [1] and [4] were determined. The sequence of glucanase [4] (30 AA residues) has a high homology to cDNA sequence of genomic DNA of rice (D40610). The sequence of glucanase [1] (five AA residues) is homorogous to cDNA expression tag of rice (D39445). The mucleotide sequence if 400 bp from 3' and 5' end of the D39445 cDNA were determined. These results serves as the basic knowledge for the future study on the regulation mechanism of auxin on cis element of the upstream of glucanase genes in elongation growth of cereal plants.

我们纯化并鉴定了5种大麦细胞壁结合的葡聚糖酶：[1]，内切-1，3；1，4-葡聚糖酶(31kD)；[2]，内切-1，3-葡聚糖酶-1(31kD)；[3]，内切-1，3-葡聚糖酶-2(31kD)；[4]，外切-葡聚糖酶-1(66kD)；[5]，外切-葡聚糖酶-2(68kD)。谷类植物中参与1，3；1，4-葡聚糖降解的酶可能是葡聚糖酶[1]和[4]。胞外葡聚糖酶-1[4]对1，3；1，4-β-葡聚糖的活性最高，占结合在细胞壁上的总葡聚糖酶活性的60%。内切葡聚糖酶[1]导致1，3；1，4-β-葡聚糖分子急剧下移。测定了葡聚糖酶[1]和[4]的N-末端氨基酸序列。葡聚糖酶[4]的序列(30个氨基酸残基)与水稻基因组DNA的cDNA序列(D40610)有很高的同源性。葡聚糖酶[1](5个氨基酸残基)序列与水稻基因表达标签(D39445)同源。测定了D39445基因3‘端和5’端400bp的核苷酸序列。这些结果为进一步研究生长素对谷类植物伸长生长中葡聚糖酶基因上游顺式元件的调控机制奠定了基础。

项目成果

桜井直樹: "植物には血管があるか" 化学と生物. 34. 24-33 (1996)

樱井直树：“植物有血管吗？” 34. 24-33 (1996)

T.Kotake,N.Nakagawa K.Takeda N.Sakurai: "Purification and characterization of wall-bound exo-1,3-β-D-glucanase from barley (Hordeum vulgaseL) seedlings" Plant and Cell Physiology. 38. 194-200 (1997)

T.Kotake、N.Nakakawa K.Takeda N.Sakurai：“大麦 (Hordeum vulgaseL) 幼苗的壁结合外切 1,3-β-D-葡聚糖酶的纯化和表征”植物和细胞生理学 38。194-。 200 (1997)

Kojima, K. and N. Sakurai: "IAA distribution in etiolated spears of asparagus" Hort Science. 29. 822-822 (1995)

Kojima, K. 和 N. Sakurai：“芦笋黄化矛中的 IAA 分布”Hort Science。

Kotake, T., N.Nakagawa, K.Takeda and N.Sakurai: "Purification and characterization of wall-bound exo-1,3-beta-D-glucanase from barley (Hordeum vulgare L.) seedlings." Plant Cell Physiol.38. 194-200 (1997)

Kotake, T.、N.Nakakawa、K.Takeda 和 N.Sakurai：“来自大麦 (Hordeum vulgare L.) 幼苗的壁结合外切 1,3-β-D-葡聚糖酶的纯化和表征。”

Yeo, U-D., W-Y. Soh, H. Tasaka, N. Sakurai, S. Kuraishi and K. Takeda: "Cell wall polysaccharides of callus and suspension-cultured cells from three cellulose-less mutants of barley (Hordeum vulgare L)" Plant Cell Physiology. 36. 931-936 (1995)

杨，U-D.，W-Y。