ESR studies on biological free radicals in pharmaceutical sciences

本项目历时3年的合作研究取得了以下重要成果，激活了药物科学中ESR的研究。1）L-波段ESRa的研究）ESR成像新仪器和新探针的开发和改进。B）L-波段ESR新测量系统，c）稳定自旋探针的定量药代动力学分析。d）L-自旋探针的ESR带监测，以预测小鼠在X射线照射下的氧化应激强度。e）在室温和液氮温度下通过L-带ESR分析Cu（II）配合物的结构。2）X-带ESRa的研究。开发ESR成像仪器。B）检测与癌症、肝病、c）通过使用ESR自旋标记和自旋探针方法，就溶血和药物代谢机制而言，表征血细胞膜中的水平3蛋白。建议在体内血液循环监测-ESR在有机自旋探针和顺磁金属离子的药代动力学分析方面。e）ESR检测金属蛋白或自由基物种的活性位点以及酶促反应过程中的中间体。f）检测制备生物活性化合物过程中的不稳定自由基中间体。g）有机硒化合物等新型抗氧化化合物的提出。

Cooperative studies on our project for 3 years gave the following important results and activated the research on ESR in pharmaceutical sciences.1) Studies on L-band ESRa) Development and improvement of new instruments as well as new probes for ESR imaging.b) New measurement system of L-band ESR,particularly for monitoring enzymatic reactions.c) Quantitative pharmacokinetic analysis of stable spin probes.d) L-band ESR monitoring of spin probes to predict the strength of oxidative stress in terms of x ray irradiation to mice.e) Structure analysis of Cu (II) complexes by L-band ESR at room temperature and liquid nitrogen temperatures.2) Studies on X-band ESRa) Developmemt of instruments for ESR imaging.b) Detection of active oxygen species and nitric oxide which relate to the development of cancer, hepatic diseases, ulcer and aging.c) Characterization of level 3 proteins in blood cell membranes in terms of hemolysis and drug metabolism in respect to the mechanism by using ESR spin label and spin probe methods.d) Proposal of in vivo blood circulation monitoring-ESR in terms of pharmacokinetic analysis of organic spin probes and paramagnetic metal ions.e) ESR detection of active site of metalloproteins or free radical species as well as intermediates during enzymatic reactions.f) Detection of unstable radical intermediates during the preparation of pharmacologically active compounds.g) Proposal of new antioxidative compounds such as organic selenium compounds.