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ESR studies on biological free radicals in pharmaceutical sciences

制药科学中生物自由基的 ESR 研究

基本信息

批准号：
07307036
负责人：
SAKURAI Hiromu
金额：
$ 2.11万
依托单位：
Kyoto Pharmaceutical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07307036/
关键词：
X-band ESR L-band ESR free radical in vitro measurement in vivo measurement spin probe spin-label metal ion L-バンドESR 常磁性金属イオン薬物動態 ESRイメージング BCM-ESR SOD

项目摘要

Cooperative studies on our project for 3 years gave the following important results and activated the research on ESR in pharmaceutical sciences.1) Studies on L-band ESRa) Development and improvement of new instruments as well as new probes for ESR imaging.b) New measurement system of L-band ESR,particularly for monitoring enzymatic reactions.c) Quantitative pharmacokinetic analysis of stable spin probes.d) L-band ESR monitoring of spin probes to predict the strength of oxidative stress in terms of x ray irradiation to mice.e) Structure analysis of Cu (II) complexes by L-band ESR at room temperature and liquid nitrogen temperatures.2) Studies on X-band ESRa) Developmemt of instruments for ESR imaging.b) Detection of active oxygen species and nitric oxide which relate to the development of cancer, hepatic diseases, ulcer and aging.c) Characterization of level 3 proteins in blood cell membranes in terms of hemolysis and drug metabolism in respect to the mechanism by using ESR spin label and spin probe methods.d) Proposal of in vivo blood circulation monitoring-ESR in terms of pharmacokinetic analysis of organic spin probes and paramagnetic metal ions.e) ESR detection of active site of metalloproteins or free radical species as well as intermediates during enzymatic reactions.f) Detection of unstable radical intermediates during the preparation of pharmacologically active compounds.g) Proposal of new antioxidative compounds such as organic selenium compounds.

我们的项目合作研究了3年，取得了以下重要成果，并激活了ESR在制药科学中的研究。(1) l波段ESRa研究)ESR成像新仪器、新探头的研制与改进。b)新的l波段ESR测量系统，特别用于监测酶反应。c)稳定自旋探针的定量药代动力学分析。d)自旋探针的l波段ESR监测，预测x射线照射小鼠氧化应激强度。e)室温和液氮温度下的l波段ESR分析Cu （II）配合物的结构。2) x波段ESRa的研究ESR成像仪器的研制。b)检测与癌症、肝脏疾病、溃疡和衰老发生相关的活性氧和一氧化氮。c)利用ESR自旋标记和自旋探针方法从溶血和药物代谢的角度表征血细胞膜上的3级蛋白的作用机制。d)基于有机自旋探针和顺磁性金属离子药代动力学分析的体内血液循环监测- esr的建议。e)酶促反应中金属蛋白或自由基活性位点及中间体的ESR检测。f)在药物活性化合物制备过程中不稳定自由基中间体的检测。g)提出新的抗氧化化合物，如有机硒化合物。