Research and Development of Protective Compounds against UV Iight-induced Skin Damage on the Basis of anti-ROS properties

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Recent increase of ultraviolet (UV) lights due to the increase of ozone hole is suggested to enhance a severe damage and premature photoaging in the skin of animals. Although the harmful effects of UV radiation on the skin are associated with the generation of reactive oxygen species (ROS), direct proof of ROS production in the skin under UV irradiation has been elusive. Recently, we have reported first an in vivo detection and imaging of the generated ROS in the skin of alive animals following UVA irradiation, where both a sensitive and specific chemiluminescent probe (CLA) and ultralow-light imaging apparatus with a CCD camera were used. Then, we proposed that superoxide anion radical (・O_2^-) is formed spontaneously and singlet oxygen (^1O_2) is generated in the UVA-irradiated skin. This method was applied to examine the relationship between ROS and aging, and find preventive agents again skin damage, and we found that ROS generation induced by UVA irradiation in the hairless rat (8-80 weeks old) skin increases age-dependently, and ascorbic acid-3-phosphate suppresses effectively oxidative stress in the skin of mice under UVA irradiation.This method is useful not only for noninvasive investigation of the spatial distribution and quantitative determination of ROS in the skin of alive animals, but for in vivo evaluation of the protective ability of free radical scavengers and antioxidants.