Protein translocation across the cytoplasmic membrane of bacteria
Protein translocation across the cytoplasmic membrane of bacteria
批准号:
07308069
负责人:
TOKUDA Hajime
金额:
$6.14万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (A)
财政年份:
1995
资助国家:
日本
项目状态:
已结题
起止时间:
1995 至 1996
中文摘要
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英文摘要
1. SecG was found to interact with SecA and facilitate the membrane insertion-deinsertion cycle of SecA.2. Mode of interaction of SecY-SecE-SecG complex was revealed.3. SecA was found to be involved in the import of cytochrome f into thylakoid.4. SecY and SecE were reconstituted into a planar lipid bilayr membrane and found to form a ion-conducting channel.5. Step-wise translocation of precursor protein was found to be caused by a hydrophobic region of the precursor.6. Structure and function of Bacillus subtilis SRP homologue were revealed.7. Highly active protein translocation system was reconstituted into proteoliposomes.
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Uchida,K.: "Stepwise Movement of Preproteins in the Process of Translocation across the Cytoplasmic Membrance of Escherichia coli." J.Biol.Chem.270. 30862-30868 (1995)
Uchida,K.:“前蛋白在大肠杆菌细胞质膜易位过程中的逐步运动。”
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Sato,K.: "Short hydrophobic segments in the mature domain of proOmpA determine its stepwise movement during translocation across the cytoplasmic membrane of Escherichia coli" J.Biol.Chem.272(in press). (1997)
Sato,K.:“proOmpA 成熟结构域中的短疏水片段决定了其在大肠杆菌细胞质膜易位过程中的逐步运动”J.Biol.Chem.272(出版中)。
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Homma, T., Yoshihisa, T., Kihara, A., Akiyama, Y., and Ito, K.: "Intracellular stability of a fragment of beta-galactosidase : effects of amino-terminally fused polypeptides." Biochem. Biophys. Res. Commun.215. 452-458 (1995)
Homma, T.、Yoshihisa, T.、Kihara, A.、Akiyama, Y. 和 Ito, K.:“β-半乳糖苷酶片段的细胞内稳定性:氨基末端融合多肽的影响”。
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Endo, T., Nakayama, Y., and Nakai, M.: "Avidin fusion protein as a tool to genecrate a stable translocation intermediate spanning the mitochondrial membranes." J.Biochem.118. 753-759 (1995)
Endo, T.、Nakayama, Y. 和 Nakai, M.:“抗生物素蛋白融合蛋白作为生成跨越线粒体膜的稳定易位中间体的工具。”
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Nishiyama,K.: "Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation." Cell. 85. 71-81 (1996)
Nishiyama,K.:“SecG 膜拓扑结构的反转与 SecA 依赖性前蛋白易位相结合。”
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共 76 条
Molecular mechanisms underlying the selective membrane localization of bacterial lipoproteins
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批准号:18K05396
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.83万
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财政年份:2018
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负责人:TOKUDA Hajime
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依托单位:
Molecular mechanisms underlying the sorting of bacterial lipoproteins.
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批准号:22380049
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$10.65万
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财政年份:2010
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负责人:TOKUDA Hajime
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依托单位:
Molecular mechanisms underlying the membrane sorting of bacterial lipoproteins
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批准号:19380046
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$12.06万
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财政年份:2007
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负责人:TOKUDA Hajime
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依托单位:
Sorting and membrane localization of E.coli lipoproteins
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批准号:15208009
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$28.12万
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财政年份:2003
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负责人:TOKUDA Hajime
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依托单位:
Molecular Mechanisms underlying membrane localization and qualify control of lipoproteins in Escherichia coli cell surface
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批准号:14037212
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项目类别:Grant-in-Aid for Scientific Research on Priority Areas
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资助金额:$82.69万
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财政年份:2002
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负责人:TOKUDA Hajime
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依托单位:
Structure and function of protein translocation and localization system
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批准号:09308021
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项目类别:Grant-in-Aid for Scientific Research (A).
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资助金额:$8.9万
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财政年份:1997
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负责人:TOKUDA Hajime
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依托单位:
Structure and function of Sec factors involving protein translocation
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批准号:07458148
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$4.54万
-
财政年份:1995
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负责人:TOKUDA Hajime
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依托单位:
Factors affecting the efficiency of protein secretion in E.coli.
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批准号:06558096
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项目类别:Grant-in-Aid for Developmental Scientific Research (B)
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资助金额:$11.97万
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财政年份:1994
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负责人:TOKUDA Hajime
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依托单位:
Reconstitution of the E.coli protein translocation machinery
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批准号:05454620
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$3.71万
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财政年份:1993
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负责人:TOKUDA Hajime
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依托单位:
Properties and physiological roles of Na^+-motive respiratory chain in marine bacteria.
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批准号:61560110
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.02万
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财政年份:1986
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负责人:TOKUDA Hajime
-
依托单位:
海外基金