Molecular mechanisms underlying the membrane sorting of bacterial lipoproteins

细菌脂蛋白膜分选的分子机制

• 批准号: 19380046
• 负责人: TOKUDA Hajime
• 金额: $ 12.06万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19380046/
• 关键词: 膜局在化; ABCトランスポーター; 分子シャペロン; ペリプラズム; リポ蛋白質; 光架橋反応; LolA; LolB; LolCDE; 選別シグナル; 大腸菌

Lipoproteins possessing the lipid-attached cysteine residue at the N-terminus are widely distributed among bacteria and anchored to membranes through the N-terminal lipids. Lipoproteins are involved in various functions present in cell surfaces and localized in either the inner or outer membrane of Gram-negative bacteria depending on their sorting signals. We have reveled that five Lol factors are involved in the membrane sorting of lipoproteins. The mechanisms underlying the specific membrane sorting of lipoproteins by Lol proteins are examined in this study.

在n端具有脂质附着半胱氨酸残基的脂蛋白广泛分布于细菌中，并通过n端脂质锚定在膜上。脂蛋白参与多种功能，存在于细胞表面，并根据其分选信号定位于革兰氏阴性菌的内膜或外膜。我们发现5个Lol因子参与了脂蛋白的膜分选。本研究探讨了Lol蛋白对脂蛋白特异性膜分选的机制。

项目成果

Introduction of a lethal redox switch that controls the opening and closing of the hydrophobic cavity in LolA.

引入致命的氧化还原开关，控制 LolA 中疏水腔的打开和关闭。

• 发表时间: 2008
• 期刊: J.Biol.Chem. 283
• 作者: Watanabe;S.;Oguchi;Y.;Takeda;K.;Miki;K.;Tokuda;H.
• 通讯作者: H.

Backbone resonance assignment for the outer membrane lipoprotein receptor LolB from Escherichia coli

大肠杆菌外膜脂蛋白受体 LolB 的骨架共振分配

• 发表时间: 2007
• 期刊: Biomol. NMR Assign. 1
• 作者: Tateishi;Y.;Ariyoshi;H.;Igarashi;R.;Hara;H.;Mizuguchi;K.;Seto;A.;Nakai;A.;Kolcubo;T.;Tochio;H. and Shirakawa;M;杉田有治;Masayuki Oda;H. Takahashi;Shingo Nakada
• 通讯作者: Shingo Nakada

Complete reconstitution of an ATP-binding cassette transporter LolCDE complex from separately isolated subunits

• DOI: 10.1111/j.1742-4658.2007.05832.x
• 发表时间: 2007-07-01
• 期刊: FEBS JOURNAL
• 影响因子: 5.4
• 作者: Kanamaru, Kyoko;Taniguchi, Naohiro;Tokuda, Hajime
• 通讯作者: Tokuda, Hajime

Diverse effects of phospholipids on lipoprotei sorting and ATP hydrolysis by the ABC transporter LolCDE complex.

磷脂对 ABC 转运蛋白 LolCDE 复合物的脂蛋白分选和 ATP 水解的多种影响。

• 发表时间: 2007
• 期刊: Biochim. Biophys. Acta. 1768
• 作者: Miyamoto;S.
• 通讯作者: S.

Large-scale preparation of the homogeneous LolA-lipoprotein complex and efficient in vitro transfer of lipoproteins to the outer membrane in a LolB-deperldent manner

大规模制备同质 LolA-脂蛋白复合物并以 LolB 依赖性方式在体外将脂蛋白有效转移到外膜

• 发表时间: 2007
• 期刊: Protein Sci 16
• 作者: Watanabe;S.
• 通讯作者: S.