Sorting and membrane localization of E.coli lipoproteins

大肠杆菌脂蛋白的分选和膜定位

• 批准号: 15208009
• 负责人: TOKUDA Hajime
• 金额: $ 28.12万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15208009/
• 关键词: protein transport; molecular chaperone; reconstitution; Escherichia coli; ABC transporter; lipoprotein; membrane localization; sorting signal

E.coli lipoproteins are sorted to the outer membrane depending on the amino acid residue located next to the amino-terminal lipid-attached cysteine. The sorting is mediated by the Lol system composed of five Lol proteins. The lipoprotein transfer reaction from LolA to LolB is unidirectional and requires no energy. This reaction was found to depend on the strength of hydrophobic interaction between lipoproteins and LolA or LolB. If the LolA-lipoprotein interaction is too strong, the transfer of lipoprotein to LolB is inhibited. Opening and closing of the hydrophobic cavity of LolA was found to be critical for the LolA function. Finally, the region of LolD important for the interaction with membrane subunits LolC and LolE was determined.

大肠杆菌脂蛋白根据位于氨基末端脂质连接的半胱氨酸旁边的氨基酸残基被分选到外膜。分选由五种Lol蛋白组成的Lol系统介导。从LolA到LolB的脂蛋白转移反应是单向的，不需要能量。发现该反应依赖于脂蛋白与LolA或LolB之间的疏水相互作用的强度。如果LolA-脂蛋白相互作用太强，则脂蛋白向LolB的转移被抑制。发现LolA疏水腔的打开和关闭对于LolA功能是至关重要的。最后，确定了LolD与膜亚基LolC和LolE相互作用的重要区域。

项目成果

Narita, S: "A Mutation in the membrane subunit of an ABC transporter LolCDE complex causing outer membrane localization of lipoproteins against their inner membrane-specificsignals"Mol.Microbiol.. 49. 167-177 (2003)

Narita, S：“ABC 转运蛋白 LolCDE 复合物的膜亚基中的突变导致脂蛋白针对其内膜特异性信号进行外膜定位”Mol.Microbiol.. 49. 167-177 (2003)

Depletion of SecDF-YajC causes a decrease in the level of SecG: implication for their functional interaction

• DOI: 10.1016/s0014-5793(03)00847-0
• 发表时间: 2003-08-28
• 期刊: FEBS LETTERS
• 影响因子: 3.5
• 作者: Kato, Y;Nishiyama, K;Tokuda, H
• 通讯作者: Tokuda, H

蛋白質輸送とエンベロープの形成(原核細胞)

蛋白质运输和包膜形成（原核细胞）

• 发表时间: 2004
• 期刊: 蛋白質・核酸・酵素 49
• 作者: Inubushi;K.;(Oslan Jumadi);徳田元
• 通讯作者: 徳田元

Takeda, K.: "Crystallization and preliminary crystallographic study of the outer membrane lipoprotein receptor LolB. a member of the lipoprotein localization factors"Acta Cryst.. D59. 1224-1226 (2003)

Takeda, K.：“外膜脂蛋白受体 LolB 的结晶和初步晶体学研究。脂蛋白定位因子的成员”Acta Cryst.. D59。

Mechanisms underlying energy-independent transfer of lipoproteins from LolA to LolB, which have similar unclosed β-barrel structures.

脂蛋白从 LolA 到 LolB 的能量依赖性转移的机制，LolB 具有类似的未封闭 β 桶结构。

• 发表时间: 2005
• 期刊: J. Biol. Chem. 280
• 作者: Taniguchi;N.
• 通讯作者: N.