Structure and function of Sec factors involving protein translocation

涉及蛋白质易位的Sec因子的结构和功能

• 批准号: 07458148
• 负责人: TOKUDA Hajime
• 金额: $ 4.54万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07458148/
• 关键词: Protein translocation; Prokaryote; Reconstitution; SecY; SecE; SecG; SecA; 大腸菌

Efficient protein translocation machinery was reconstituted from SecA,SecY,SecE and SecG into proteoliposomes. SecG was found to undergo the inversion cycle of membrane topology during protein translocation. This cycle was found to be coupled with the membrane insertion and deinsertion cycle of SecA.

将SecA、SecY、SecE和SecG重组为蛋白脂质体。SecG被发现在蛋白质易位过程中经历膜拓扑结构的反转循环。发现该循环与SecA的膜插入和去插入循环相结合。

项目成果

Tokuda,H.: "Protein traffic from the cytosol to the outer membrane of Escherichia coli." Protein targetting. (in press). (1997)

Tokuda, H.：“大肠杆菌从细胞质到外膜的蛋白质运输。”

Tokuda, H.: "Topology inversion of SecG,a membrane factor of E.coli protein translocase" Membrane Proteins : "Structure, function and expression control" (ed.N.Hamasaki and K.Mihara, Kyusyu University Press/Karger Medical and Scientific Publishers). 183-1

Tokuda, H.：“SecG 的拓扑反转，大肠杆菌蛋白转位酶的膜因子”膜蛋白：“结构、功能和表达控制”（ed.N.Hamasaki 和 K.Mihara，九州大学出版社/Karger Medical and

Kontinen,V.P.: "The secG deletion mutation of Escherichia coli is suppressed by expression of a novel regulate gene of Bacillus subtilis." FEBS Lett.389. 281-284 (1996)

Kontinen，V.P.：“枯草芽孢杆菌的新型调节基因的表达可抑制大肠杆菌的 secG 缺失突变。”

Kontinen V.P., and Tokuda, H.: "Overexpression of phosphatidylglycerophosphate synthase restores protein translocation in a secG deletion mutant of Escherichia coli at low temperature" FEBS Lett.364. 157-160 (1995)

Kontinen V.P. 和 Tokuda, H.：“磷脂酰甘油磷酸合酶的过度表达可在低温下恢复大肠杆菌 secG 缺失突变体中的蛋白质易位”FEBS Lett.364。

Hirano, M., Matsuyama, S., and Tokuda, H.: "The carboxyl-terminal region is essential for SecA dimerization." Biochem. Biophys. Res. Commun.229. 90-95 (1996)

Hirano, M.、Matsuyama, S. 和 Tokuda, H.：“羧基末端区域对于 SecA 二聚化至关重要。”