DESIGNING OF HEALTHFUL ENVIRONMENT FOR HUMAN LIFE-A BASIC STUDY TO CLARIFY THE PREVENTIVE EFFECT OF AN ANTI-STREES VITAMIN,VITAMIN C ON THE COMMON CHRONIC DISEASE OF ADULT PEOPLE

L-抗坏血酸，又称维生素C，是一种水溶性抗氧化剂，也是灵长类动物的必需营养物质，因为它们缺乏生物合成。被动吸烟暴露在香烟的侧流烟雾中，据说会增加不吸烟者患某些疾病的风险，因为香烟的侧流烟雾比主流香烟烟雾含有更多对身体有害的有机化合物和自由基。本研究从被动吸烟的角度出发，研究了AsA代谢和药物代谢酶的活性，以阐明AsA对香烟烟雾化学应激的生物防御作用。将合成阿司匹林的Wistar雄性大鼠和不合成阿司匹林的阿司匹林大鼠分为对照组(C组)和试验组(T组)。T组动物暴露于侧流烟雾中，每天2小时。实验结束后，用高效毛细管电泳法测定大鼠肝脏和其他组织中AsA的浓度。香烟烟雾暴露后，Wistar大鼠和ods大鼠各组织中AsA水平和药物代谢酶活性均升高。推测ASA是在大鼠体内合成的，当ASA作为抗氧化剂与某些自由基反应或支持提高药物代谢酶的活性以对抗化学应激时。在香烟烟雾暴露的ODS大鼠中，观察到各种组织中ASA含量的意外增加，这种明显的增加可能是由于ASA代谢对香烟烟雾所致的化学应激的某种适应的结果。因此，被动吸烟对大鼠和消耗臭氧层物质大鼠体内的ASA代谢和药物代谢酶活性均有较大影响。

L-ascorbic acid (AsA), known as Vitamin C is a water-soluble antioxidant and an essential nutrient for the primate, because of their deficiency to biosynthesize it. Passive smoking, exposed to sidestream smoke from cigarette, is said to increase the risk of certain disease in nonsmokers, since sidestream from cigarette have much more harmful organic compounds and free radicals for body than mainstream smoke from cigarette. In this study, in order to clarify the bio-defense function of AsA against chemical stress from cigarette smoke, the AsA metabolism and the activities of drug-metabolizing enzyme were studied from the standpoint of the effect of passive smoking. Wistar male rats, which synthesized AsA,and ODS rats, which chould not synthesize AsA,were divided into two group, the control (C group) and the test (T group) groups. The animals in T group were exposed to sidestream smoke from cigarette for 2 hours everyday. After the experimental period, the AsA concentration in liver, and other tissues were measured by HPLC.When exposed to cigarette smoke, AsA levels in various tissues and activities of drug-metabolizing enzymesincreased in Wistar rats and ODS rats. It was speculated that AsA was synthesized in rats, when AsA was consumed to react with some radicals as an antioxidant or to support in raising the activities of drug-metabolizing enzymes against chemical stress. In the case of ODS rats exposed to cigarette smoke, an unexpected increase of AsA contents in various tissues was observed, and this apparent increase might be the result of some adaptation in AsA metabolism against chemical stress due to cigarette smoke. Therefore, it was concluded that the passive smoking gave a considerable effect on both AsA metabolism and activities of drug-metabolizing enzymes in rats and ODS rats.