Protein phosphorylation by cellular networks including plant MAP-kinase cascades

包括植物 MAP 激酶级联在内的细胞网络的蛋白质磷酸化

基本信息

  • 批准号:
    07308072
  • 负责人:
  • 金额:
    $ 3.07万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
  • 财政年份:
    1995
  • 资助国家:
    日本
  • 起止时间:
    1995 至 1996
  • 项目状态:
    已结题

项目摘要

Machida : Protein kinase NPK1 that was identified in tobacco is homolohous in terms of an amino acid sequence to members of the mitogen-activated protein kinase kinase kinase (MAPKKK) family, and NPK1 cDNA can complement a mutation of BCK1 gene, one of the yeast homologue of MAPKKK.In the present study, a cDNA for an activator of NPK1 was isolated by using an activatioin cloning system with yeast cells. Structural and immunological analyzes with the cDNA showed that it codes for a new member of a kinesin-like protein because the predicted protein has an amino acid sequence at the amino terminus that is highly homologous to that of the motor domain of the kinesin super family. The predicted protein was designated NPK1-activating kinesisn-like protein (NACK). When both NPK1 and NACK cDNAs were overexpressed in yease cells, activity of NPK1 increased by approximately 10-fold by the interaction of the carboxy-terminal region of NPK1 with the middle of the stalk region of NACK.NACK was accu … More mulated only at the M phase of the cell cycle, and NPK1 was detected from the S phase to the end of M phase. These results suggest that NPK1 can be activated at the M phase and function in a process of that phase. Shinozaki : cDNAs for nine homologues of MAP kinases, cDNAs for three homologues of MAPKK,cDNAs for four homologues of MAPKKK,cDNAs for two homologues of S6 protein kinase were cloned. Genes for some of the protein kinases (ATMPK3, ATMEKK,ATPK19) were shown to be expressed in response to drought, cold temperature and salt stress, suggesting that they constitute a single signal cascade. Oka : The results of experiments with transgenic Arabidopsis plants that have its homologue of PP1 (protein phosphatase 1) suggest that the homologue functions in positive response to ethylene. Nakamura : A 54-kDa protein kinase in tobacco leaves was activated by the treatment with sucrose, and it was shown to be a member of the CDPK family. Izui : PEP carboxylase of maize was shown to be phosphorylated by a member of CDPK.Complementary DNAs for candidate protein kiases that may be involved in such phosphorylation were cloned. Less
町田:在烟草中发现的蛋白激酶NPK 1与丝裂原活化蛋白激酶(MAPKKK)家族成员的氨基酸序列同源,并且NPK 1 cDNA可以互补MAPKKK的酵母同源物BCK 1基因的突变。与cDNA的结构和免疫学分析表明,它编码的驱动蛋白样蛋白的一个新成员,因为预测的蛋白质具有在氨基末端的氨基酸序列是高度同源的驱动蛋白超家族的马达结构域。预测的蛋白质被命名为NPK 1激活的运动蛋白样蛋白(NACK)。当NPK 1和NACK cDNA在酵母细胞中过表达时,NPK 1的活性通过NPK 1的羧基末端区域与NACK的茎部区域的中间的相互作用而增加约10倍。 ...更多信息 NPK 1仅在细胞周期的M期表达,从S期到M期末均有表达。这些结果表明,NPK 1可以在M期被激活,并在该阶段的过程中发挥作用。筱崎:克隆了9个MAP激酶同源物、3个MAPKK同源物、4个MAPKKK同源物和2个S6蛋白激酶同源物的cDNA。一些蛋白激酶(ATMPK 3,ATMEKK,ATPK 19)的基因被证明是在响应干旱,低温和盐胁迫的表达,这表明它们构成了一个单一的信号级联。Oka:用具有PP 1(蛋白磷酸酶1)同源物的转基因拟南芥植物进行的实验结果表明,该同源物在对乙烯的正响应中起作用。中村:烟草叶片中的一种54-kDa蛋白激酶通过蔗糖处理被激活,并且被证明是CDPK家族的成员。出井:玉米PEP羧化酶被CDPK的一个成员磷酸化,克隆了可能参与这种磷酸化的候选蛋白激酶的互补DNA。少

项目成果

期刊论文数量(31)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Ryuichi Nishihara: "Possible involvent of differential splicing in the regulation of the activity of Arabidopsis ANP1 that is related to mitogen-activated protein kinase kinase kinases(MAPKKKs)" The Plant Journal. (印刷中). (1997)
Ryuichi Nishihara:“可能涉及拟南芥 ANP1 活性调节中与丝裂原激活蛋白激酶激酶 (MAPKKK) 相关的差异剪接”植物杂志(1997 年出版)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Shoji Usami: "Cutting activates a 46-kilodalton protein kinase in plants" Proceedings National Academy of Science USA. 92. 8660-8664 (1995)
Shoji Usami:“切割激活植物中 46 千道尔顿的蛋白激酶”美国国家科学院院刊。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Norihiro Ohtsubo: "Cooperation of two distinct cis-acting elements is necessary for the S phase-specific activation of the wheat hisone H3 promoter." The Plant Journal. (印刷中). (1997)
Norihiro Ohtsubo:“两个不同的顺式作用元件的合作对于小麦组蛋白 H3 启动子的 S 期特异性激活是必要的。”(植物杂志)(1997 年)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Yusuke Saijo: "cDNA cloning and prokaryotic expression of maize calcium-dependent protein kinases" Biochim Biophy Acta. (印刷中). (1997)
Yusuke Saijo:“玉米钙依赖性蛋白激酶的 cDNA 克隆和原核表达”Biochim Biophy Acta(出版中)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Tsuyoshi Furumoto et al.: "Plant calcium-dependent protein kinase-related kinases (CDPKs) do not require calcium for their activities." FEBS letters. 396. 147-151 (1996)
Tsuyoshi Furumoto 等人:“植物钙依赖性蛋白激酶相关激酶 (CDPK) 的活性不需要钙。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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MACHIDA Yasunori其他文献

MACHIDA Yasunori的其他文献

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{{ truncateString('MACHIDA Yasunori', 18)}}的其他基金

Correlation between asymmetric DNA replication and asymmetric cell division
不对称DNA复制与不对称细胞分裂之间的相关性
  • 批准号:
    25650094
  • 财政年份:
    2013
  • 资助金额:
    $ 3.07万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Mechanisms of M phase progression and cytokinesis of plant cells: a relationship to asymmetric cell division
植物细胞 M 期进展和胞质分裂的机制:与不对称细胞分裂的关系
  • 批准号:
    23370021
  • 财政年份:
    2011
  • 资助金额:
    $ 3.07万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular mechanisms controlled by cytokinin ofM phase progression in division cycle at tobacco BY-2 cells
烟草 BY-2 细胞分裂周期中细胞分裂素 ofM 期进展控制的分子机制
  • 批准号:
    17370016
  • 财政年份:
    2005
  • 资助金额:
    $ 3.07万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular Mechanisms Controlling Tissue and Organ Differentiation
控制组织和器官分化的分子机制
  • 批准号:
    10182102
  • 财政年份:
    1998
  • 资助金额:
    $ 3.07万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas (A)
Molecular interaction between Agrobacterium and plant cells
农杆菌和植物细胞之间的分子相互作用
  • 批准号:
    05454017
  • 财政年份:
    1993
  • 资助金额:
    $ 3.07万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Molecular bases of initial processes for formation of crown gall tumor
冠瘿瘤形成初始过程的分子基础
  • 批准号:
    02404003
  • 财政年份:
    1990
  • 资助金额:
    $ 3.07万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (A)
Molecular Mechanism of Crwon Gall Tumor Formation (Transfer of T-DNA into Monocotyledonous plants)
Crwon瘿瘤形成的分子机制(T-DNA转移到单子叶植物中)
  • 批准号:
    63480013
  • 财政年份:
    1988
  • 资助金额:
    $ 3.07万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Molecular mechanisms of T-DNA transfer from Agrobacterium to plant cells during formations of crown gas tumor.
冠气瘤形成过程中 T-DNA 从农杆菌转移到植物细胞的分子机制。
  • 批准号:
    60480011
  • 财政年份:
    1985
  • 资助金额:
    $ 3.07万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

相似海外基金

キネシン様タンパク質によるタバコキナーゼNPK1(MAPKKK)の活性化と細胞分裂
驱动蛋白样蛋白激活烟草激酶 NPK1 (MAPKKK) 和细胞分裂
  • 批准号:
    97J02003
  • 财政年份:
    1998
  • 资助金额:
    $ 3.07万
  • 项目类别:
    Grant-in-Aid for JSPS Fellows
NPK1キナーゼとキネシン様蛋白質及び微小管による植物細胞の増殖制御の研究
NPK1激酶、类驱动蛋白和微管控制植物细胞生长的研究
  • 批准号:
    08454256
  • 财政年份:
    1996
  • 资助金额:
    $ 3.07万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
植物細胞の増殖と分化におけるNPK1プロテインキナーゼの役割
NPK1蛋白激酶在植物细胞增殖和分化中的作用
  • 批准号:
    07254205
  • 财政年份:
    1995
  • 资助金额:
    $ 3.07万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
植物細胞の増殖と分化におけるNPK1プロテインキナーゼの役割
NPK1蛋白激酶在植物细胞增殖和分化中的作用
  • 批准号:
    06262212
  • 财政年份:
    1994
  • 资助金额:
    $ 3.07万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
植物細胞の増殖と分化におけるNPK1プロテインキナーゼの役割
NPK1蛋白激酶在植物细胞增殖和分化中的作用
  • 批准号:
    05269212
  • 财政年份:
    1993
  • 资助金额:
    $ 3.07万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
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