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Molecular Mechanisms Controlling Tissue and Organ Differentiation

控制组织和器官分化的分子机制

基本信息

批准号：
10182102
负责人：
MACHIDA Yasunori
金额：
$ 180.48万
依托单位：
NAGOYA UNIVERSITY (1999-2002)Kyoto University (1998)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas (A)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2002
项目状态：
已结题

项目摘要

Following the research plan proposed initially, the group of these investigators have obtained results described below. Machida isolated and characterized the ASYMMETRIC LEAVES2(AS2) and AS1 genes. Proteins that are encoded by these genes were shown to be associated each other and to act as transcription repressors against expression of class1 knox homeobox genes during leaf development. Tsukaya cloned the AN gene that is responsible specifically for lateral expansion of leaf lamina and that demonstrated that AN protein is similar in terms of amino acid sequence to CtBP(C-terminal binding protein). His group also showed that AN controls structures of cell walls through modulation of formation of cortical microtubules of plant cells. Nakamura isolated a number of Arabidopsis mutants in which sugar-dependent expression of the SPO : : LUC reporter gene is affected by the mutations. These mutants were shown to include ones that exhibited only an abnormal sugar response and ones that also altered responses to ABA as well as sugar. Thus, there must be two independent pathways for these responses. Shimamoto attempted to identify genes that may determine the short-day response of flowering of rice and showed that the rice homolog of the CO gene represses expression of the rice homolog of the FT gene, which is responsible for short-day flowering of rice. Umeda demonstrated that CDKB2/cycline B complex is involved directly in differentiation of rice cells as well as progression of cell cycle. He also showed that a rice CAK has an ability to form calli without cytokinin in vitro. Nishitani showed that Arabidopsis has 33 gene that belong to the EXGT family and that these genes are controlled in individual manners for tissue-specific expression of these genes. Our group including members who had been selected from general applicants has provided sufficient contributions to morphogenesis and differentiation of plants.

根据最初提出的研究计划，这些研究人员小组获得了下述结果。町田分离并鉴定了不对称叶2（AS 2）和AS 1基因。由这些基因编码的蛋白质被证明是相互关联的，并作为转录抑制剂对class 1诺克斯同源异型盒基因的表达在叶发育过程中。Tsukaya克隆了AN基因，该基因专门负责叶片的侧向扩展，并证明AN蛋白在氨基酸序列方面与CtBP（C-末端结合蛋白）相似。他的研究小组还表明，AN通过调节植物细胞皮层微管的形成来控制细胞壁的结构。中村分离了许多拟南芥突变体，其中SPO：：LUC报告基因的糖依赖性表达受到突变的影响。这些突变体被证明包括那些只表现出异常的糖反应和那些也改变响应阿坝以及糖。因此，这些反应必须有两个独立的途径。Shimamoto试图鉴定可能决定水稻开花的短日照反应的基因，并表明CO基因的水稻同源物抑制FT基因的水稻同源物的表达，FT基因负责水稻的短日照开花。Umeda等人证明CDKB 2/cycline B复合物直接参与水稻细胞的分化和细胞周期的进程。他还表明，水稻CAK在体外没有细胞分裂素的情况下具有形成愈伤组织的能力。Nishitani表明拟南芥有33个属于EXGT家族的基因，并且这些基因以个体方式被控制以使这些基因的组织特异性表达。我们的研究小组包括从普通申请人中选出的成员，他们对植物的形态建成和分化做出了足够的贡献。