Mechanisms of M phase progression and cytokinesis of plant cells: a relationship to asymmetric cell division

1.我们已经在烟草和拟南芥中分别鉴定了蛋白磷酸酶的NA2C和AtNA2C基因，它们可能参与胞质分裂所需的NACK-PQR途径的激活。我们最近的研究结果表明，AtNA2C与分生组织细胞的不对称分裂有关。2.为了了解拟南芥GIG-APC通路在细胞命运决定中的作用，我们寻找了可能改变Gig1表型的新突变体。我们已经确定了11个新的突变。我们的研究结果表明，GIG1和UVI4是GIG1的同源基因，它们都参与气孔的发育。3.由于我们未能复制MAP65基因家族成员在拟南芥根细胞不对称分裂中的参与，我们推迟了上述两条途径中基因的双突变实验。

1. We have identified NA2C and AtNA2C genes for protein phosphatases in tobacco and arabidopsis, respectively, which may be involved in activation of the NACK-PQR pathways that are required for cytokinesis. Results of our recent studies have suggested that AtNA2C is related to asymmetric cell division of meristematic cells. 2. For understanding of a role of the GIG-APC pathway of arabidopsis in cell fate determination, we have sought for new mutants that might modify gig1 phenotypes. We have identified 11 novel mutations. Results of our investigations suggest that GIG1 and UVI4, a homolg of GIG1, both are involved in development of stomata. 3. Since we failed to reproduce the involvement of members of the MAP65 gene family in asymmetric cell division in arabidopsis roots, we postponed experiments with double mutants of genes in the two pathways described above.