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Molecular interaction between Agrobacterium and plant cells

农杆菌和植物细胞之间的分子相互作用

基本信息

批准号：
05454017
负责人：
MACHIDA Yasunori
金额：
$ 4.29万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454017/
关键词：
Agrobacterium Ti plasmid protein kinase vir genes phenol monosaccharides

项目摘要

Crown gall tumor is incited on dicotyledonous plants by infection with Agrobacterium tumefaciens harboring Ti plasmid. Upon infection, expression of most of vir genes on the Ti plasmid is induced or enhanced in the bacterial cells.Some of the vir gene products are involved in transfer of T-DNA which is transferred. We have previously found that monosaccharides such as glucose and galactose can enhance signals initiated by plant phenolic compounds. In the present study, we demonstrated that the signal by such monosaccharides is transduced into cytoplasm through the sensor protein VirA.In addition, the periplasmic domain of VirA was shown to be responsible for signal perception by the sugars and the linker domain and the region of the C-terminal side of the TM2 domain are shown to be involved in signaling of phenolics. In addition to these investigations, we have examined whether infection of plants with Agrobacterium cells activates a certain protein kinase. Our results showed that a novel protein kinase which has molecular mass of 46 kDa was transiently activated by infection of leaf discs of tobacco. Activities of 46-kDa kinases were detected in a wide variety of plant species after wounding, indicating that this protein kinase is present in a number of plants. It should be important to investigate physiological relationship between infection with Agrobacterium and function of this kinase.

用携带Ti质粒的根癌农杆菌感染双子叶植物可诱发冠瘿瘤。感染后，Ti质粒上的大部分vir基因在细菌细胞中的表达被诱导或增强，部分vir基因产物参与T-DNA的转移。我们以前已经发现单糖如葡萄糖和半乳糖可以增强植物酚类化合物引发的信号。在本研究中，我们证明了信号由这样的单糖被转导到细胞质中通过传感器蛋白VirA。此外，VirA的周质域被证明是负责信号感知的糖和连接结构域和区域的TM 2结构域的C-末端侧被证明参与信号的酚。除了这些调查，我们已经研究了是否感染植物与农杆菌细胞激活某种蛋白激酶。我们的研究结果表明，一种新的蛋白激酶，其分子量为46 kDa的瞬时激活的烟草叶盘感染。46-kDa激酶的活性被检测到在各种各样的植物物种受伤后，表明这种蛋白激酶是存在于一些植物。因此，研究农杆菌侵染与该激酶功能之间的生理关系具有重要意义。