Molecular neuroendocrine basis of spawning migration in salmon

鲑鱼产卵洄游的分子神经内分泌基础

基本信息

批准号：
07404046
负责人：
ANDO Hironori
金额：
$ 24.06万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1998
项目状态：
已结题

项目摘要

The spawning migration is a kind of innate behavior which is essential for successful reproduction in salmon. To realize a genetic program of the behavior. we have examined molecular basis of neuro-endocrine mechanisms of salmon migration. Our research has been focused mainly on the following three points ; 1)Changes in expression levels of hypothalamic and pituitary hormone genes during spawning migration, 2)Structural analysis of the neurohormone genes. 3)Roles of gonadotropin-releasing hormone (GnRH) in the control of spawning behavior. The results of this project are summarized as follows.1. The levels in neurohypophysial hormone (NH) mRNAs decrease in the pre-spawning females. The sexual dimorphic expression of the NH genes suggests that the NH may be involved in reproductive behavior as well as osmotic regulation in the homing salmon. The gonadal and adrenocortical stroid hormones may be concerned in the control of the NH gene expression.2. The expression of salmon GnRH (sGnRH) gene in the preoptic area increases during final maturation. The 5' upstream regions of the two sGnRH genes (sGnRH-I and-II) are distinctively divergent. The expression of gonadotropin alpha and IIbeta genes also. increases temporally in the final maturation, and is stimulated by the administration of genre.3. The adminiatration of GnRH shortens duration of the homing migration. The GnRH may play roles in the stimulation of not only gonadal maturation but also migratory behavior.The molecular neuroendocrine basis of spawning migration is considered to be mostly established at present. In the new project ''Moleclular neuroendocrine regulation of spawning migration in salmon", which is supported by the Grant-in-Aid for Specially Promoted Research, further investigations on the molecular mechanisms of neurohormone action in the control of salmon spawning migration will be progressively done.

产卵迁移是一种天生的行为，对于成功繁殖鲑鱼至关重要。实现行为的遗传程序。我们研究了鲑鱼迁移的神经内分泌机制的分子基础。我们的研究主要集中在以下三点上。 1）在产卵迁移过程中，下丘脑和垂体激素基因的表达水平变化，2）神经激素基因的结构分析。 3）促性腺激素释放激素（GNRH）在控制产卵行为中的作用。该项目的结果总结如下1。前产卵女性的神经型激素（NH）mRNA的水平降低。 NH基因的性二态表达表明，NH可能参与养殖鲑鱼中的生殖行为以及渗透调节。性腺和肾上腺皮质型链肌激素可能与NH基因表达的控制有关。2。在最终成熟期间，鲑鱼GnRH（SGNRH）基因的表达增加。两个SGNRH基因（SGNRH-I和-II）的5'上游区域是明显不同的。促性腺激素α和IIBETA基因的表达。在最终成熟度中暂时增加，并通过类型的施用刺激3。 GNRH的管理缩短了归巢迁移的持续时间。 GNRH不仅可以刺激性腺成熟，还可以刺激迁移行为。在新的项目“鲑鱼产卵迁移的分子神经内分泌调节”中，这是由赠款中的赠款对特殊促进研究的支持，对控制鲑鱼产卵迁移的神经激素作用的分子机制的进一步研究将逐步完成。