Studies on the Pathogenesis of Trophoblastic Tumors by DNA Fingerprint and its Relationship With Growth Factor

DNA指纹图谱研究滋养细胞肿瘤发病机制及其与生长因子的关系

• 批准号: 01480394
• 负责人: KOYAMA Masayasu
• 金额: $ 3.58万
• 依托单位: Osaka University Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480394/
• 关键词: DNA fingerprint; Hydatidiform mole; Choriocarcinoma; Androgeners; Mitochondrial DNA; Macrophage colony-stimulating factor; ミニサテライトDNAプロ-ブ; サザンブロットハイブリダイゼ-ション; ノ-ザンブロットハイブリダイゼ-ション; MCSF; c-fms

We have investigated the pathogenesis of trophoblastic tumors by analyzing restriction fragment length polymorphisms (RFLPs) using a minisatellite DNA probe (DNA fingerprint) to complete hydatidiform molecules, riocarcinoma, and hydrotropic abortus. The polymorphic fragments of the complete mole were all transmitted from the paternal DNA, while some of the polymorphic bands of paternal DNA were not observed in molar DNA (placenta 1989, 10, pp399). On the other hand, the molar mitochondrial DNA was inherited from maternal ovum (Gynecol Oncol 1991, 40, pp29). Our results verify that complete molecule results from the fertilization of an anuclear empty ovum with normal sperm at the molecular level. In the hydrotropic abortus, the polymorphic fragments of the DNA fingerprint could be traced back to its parent, indicating that the DNA fingerprint could be a useful tool for differential diagnosis between complete molecules and hydrotropic abortus (Am J Obstet Gynecol 1990, 163, pp634).Intere … More stingly, in some cases of choriocarcinoma, the pattern of inheritance of RFLPs was the same as that in the complete molecule, Whereas in others all the polymorphic fragments in tumor tissues were identical to those in the host DNA (Cancer Res 1990, 50, pp488). These results suggest that the pathogenesis of choriocarcinoma varies, being completely different from that of the complete hydatidiform mole in some cases.We also investigated the gene expression of the macrophage colony-stimulating factor (M-CSF) and its receptor, c-fms proto-oncogene, in human placenta and decidua in order to clarify the role of this growth factor in the proliferation of trophoblasts. M-CSF and c-fms mRNAs, 4.7kb and 3.9kb respectively, were detected by Northern blot hybridization in the early stage placenta and subsequently increased during pregnancy. These results indicate that M-CSF is deeply involved in the local proliferation and differentiation of trophoblasts and the auto- or paracrine system of this growth factor may play a key role of the growth of trophoblastic tumors (Am J Reprod Immunol 1990, 24, pp99). Less

我们通过使用小卫星DNA探针（DNA指纹）分析限制性片段长度多态性（RFLP）来完成葡萄状分子、里奥癌和嗜水性流产，从而研究了滋养细胞肿瘤的发病机制。完整葡萄胎的多态性片段均由父本DNA遗传而来，而部分父本DNA的多态性条带在葡萄胎DNA中未观察到(placenta 1989, 10, pp399)。另一方面，摩尔线粒体DNA是从母体卵子继承的（Gynecol Oncol 1991, 40, pp29）。我们的结果证实，完整的分子是由无核空卵子与正常精子在分子水平上受精产生的。在嗜水性流产中，DNA 指纹的多态性片段可以追溯到其亲本，这表明 DNA 指纹可能是区分诊断完整分子和嗜水性流产的有用工具（Am J Obstet Gynecol 1990, 163, pp634）。有趣的是，在某些绒毛膜癌病例中，DNA 指纹的模式 RFLP 的遗传与完整分子中的遗传相同，而在其他肿瘤组织中，所有多态性片段与宿主 DNA 中的多态性片段相同（Cancer Res 1990, 50, pp488）。这些结果表明，绒毛膜癌的发病机制各不相同，有些病例与完全性葡萄胎的发病机制完全不同。我们还研究了巨噬细胞集落刺激因子（M-CSF）及其受体c-fms原癌基因在人胎盘和蜕膜中的基因表达，以阐明该生长因子在滋养细胞增殖中的作用。通过 Northern blot 杂交在早期胎盘中检测到 M-CSF 和 c-fms mRNA 分别为 4.7kb 和 3.9kb，随后在妊娠期间增加。这些结果表明，M-CSF深入参与滋养细胞的局部增殖和分化，并且该生长因子的自分泌或旁分泌系统可能在滋养细胞肿瘤的生长中发挥关键作用(Am J ReprodImmunol 1990, 24, pp99)。较少的

项目成果

Chihiro Azuma: "Zygosity Determination of multiple pregnancy by DNA fingerprinting" Am.J.Obstet.Gynec.160. 734-736 (1989)

Chihiro Azuma：“通过 DNA 指纹识别多胎妊娠的合子性测定”Am.J.Obstet.Gynec.160。

Chihiro Azuma: "Studies on the pathogenesis of choriocarcinoma by analysis of restriction fragment length polymorphisms" Cancer Research. 50. 488-491 (1990)

Chihiro Azuma：“通过限制性片段长度多态性分析研究绒毛膜癌的发病机制”癌症研究。

東 千尋: "産婦人科領域への分子生物学の応用 ーーバイオテクノロジ-を用いた 診断と治療ーー DNA指紋を用いた多胎の卵性診断" 産科と婦人科. 56. 2377-2382 (1989)

Chihiro Higashi：“分子生物学在妇产科领域的应用 - 使用生物技术进行诊断和治疗 - 使用 DNA 指纹诊断多卵巢” 妇产科 56. 2377-2382 (1989)。

Chihro Azuma: "Steroid hormones induce macrophage colony stimulating factor (MCSF) and MCSF receptor mRNAs in the human endometrium" Gynecologic Oncology. 5. 103-108 (1990)

Chihro Azuma：“类固醇激素在人子宫内膜中诱导巨噬细胞集落刺激因子 (MCSF) 和 MCSF 受体 mRNA”《妇科肿瘤学》。

M.Mochizuki,R.Hussa(eds.): "Placental Protein Hormones. Analysis of restriction fragment length polymorphism in trophoblastic tumors." Excerpa Medica., 335 (1988)

M.Mochizuki,R.Hussa（编）：“胎盘蛋白激素。滋养层肿瘤中限制性片段长度多态性的分析。”