Screening and breeding of high CO2 fixation ability tree speceis

从九州地区优势树种中选择苦楝作为固定CO2能力最强的树种。从九州5个县采集苦楝种子，进行原性试验。利用RAPD分析揭示了这些种子的遗传变异，并通过田间遮荫和施肥试验揭示了这些种子的生长特性。介绍了苦楝的微繁繁殖方法。以刺槐为材料，进行了突变植株愈伤组织选择试验。作为这项研究的结果，以下事情是明确的。在选取的区域或个体间存在大量的遗传变异。RAPD分析结果显示，遗传距离与采集区的距离成比例较长，尤以屋久岛和种子岛与其他地区差异较大。苦楝具有太阳植物的特点，对营养物质的需求量很大。微繁殖法是苦楝的可行繁殖方法。无性系间繁殖速率存在差异。建立了一种以假金丝桃愈伤组织为材料培育新植株的技术。建立了一种利用蛋白质分析检测新植物差异的技术。通过蛋白质分析揭示了盐处理愈伤组织产生的新植株的遗传变异。

Melia azedarach was selected from tree species dominated in Kyushu area as a species which had the highest CO2 fixation ability. Seeds of M.azedarach were collected from five districts of kyushu, and were used for projeny test. The heredity variations of these seeds were revealed by RAPD analysis, and the growth characteristics were revealed by field shade and fertilizer experiments. The micropropagation method was introduced for the propagation of M.azedarach. Callus selection for producing mutant plants were tested to Robinia pseudoacassia. As a result of this study, following things were made clear.1. There were a great deal of heredity varieties among dollected district or individuals of M.azedarach.2. RAPD analysis revealed that the heredity distance were longer in proportion to the distance of collected district, especially Yakushima and Tanegasima were much different from other districts.3. M.azedarach had the characteristics of sun plant, and demanded many nutrients.4. Micropropagatin method was possible for the propagation of M.azedarach. but there were a difference for propagation rate among clones.5. A technique for producing new plants from the callus of R.pseudoaccasia was established.6. A technique for detecting the different of new plants was established by using protein analysis.7. Heredity varietions of new plants produced from salt treated callus were revealed by protein analysis.