Screening and breeding of high CO2 fixation ability tree speceis

苦楝是从九州地区的主要树种中选出的具有最高CO2固定能力的树种。从九州五个地区采集苦楝种子，并用于项目试验。通过RAPD分析揭示了这些种子的遗传变异，并通过田间遮荫和施肥试验揭示了这些种子的生长特性。介绍了苦苣苔的微繁殖方法。以刺槐为材料，进行了愈伤组织筛选和突变体产生的研究。通过这项研究，以下几点得到了明确。苦楝在不同地区或个体间存在着大量的遗传变异. RAPD分析表明，遗传距离与采集地的距离成正比，尤其是屋久岛和谷岛与其他地区的遗传距离差异较大.苦楝具有阳生植物的特性，对营养的要求较高.微繁技术是苦楝繁殖的可行方法。但无性系间的繁殖率存在差异.建立了从假红杜鹃愈伤组织诱导新植株的技术.建立了利用蛋白质分析技术检测新植物差异的技术.通过蛋白质分析，揭示了盐处理愈伤组织产生的新植株的遗传变异。

Melia azedarach was selected from tree species dominated in Kyushu area as a species which had the highest CO2 fixation ability. Seeds of M.azedarach were collected from five districts of kyushu, and were used for projeny test. The heredity variations of these seeds were revealed by RAPD analysis, and the growth characteristics were revealed by field shade and fertilizer experiments. The micropropagation method was introduced for the propagation of M.azedarach. Callus selection for producing mutant plants were tested to Robinia pseudoacassia. As a result of this study, following things were made clear.1. There were a great deal of heredity varieties among dollected district or individuals of M.azedarach.2. RAPD analysis revealed that the heredity distance were longer in proportion to the distance of collected district, especially Yakushima and Tanegasima were much different from other districts.3. M.azedarach had the characteristics of sun plant, and demanded many nutrients.4. Micropropagatin method was possible for the propagation of M.azedarach. but there were a difference for propagation rate among clones.5. A technique for producing new plants from the callus of R.pseudoaccasia was established.6. A technique for detecting the different of new plants was established by using protein analysis.7. Heredity varietions of new plants produced from salt treated callus were revealed by protein analysis.