Screening and breeding of high CO2 fixation ability tree speceis

高固碳树种的筛选与选育

• 批准号: 07456076
• 负责人: SAITO Akira
• 金额: $ 4.42万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07456076/
• 关键词: Melia azedarach; CO2 fixation; RAPD; Micropropagation; Callus selection; Salt toleraaance; ニセアカシア; 大量増殖; SDSゲル電気泳動法; 炭酸ガス; 樹木; 遺伝; 育種; 遺伝育種; 成長量; 光合成

Melia azedarach was selected from tree species dominated in Kyushu area as a species which had the highest CO2 fixation ability. Seeds of M.azedarach were collected from five districts of kyushu, and were used for projeny test. The heredity variations of these seeds were revealed by RAPD analysis, and the growth characteristics were revealed by field shade and fertilizer experiments. The micropropagation method was introduced for the propagation of M.azedarach. Callus selection for producing mutant plants were tested to Robinia pseudoacassia. As a result of this study, following things were made clear.1. There were a great deal of heredity varieties among dollected district or individuals of M.azedarach.2. RAPD analysis revealed that the heredity distance were longer in proportion to the distance of collected district, especially Yakushima and Tanegasima were much different from other districts.3. M.azedarach had the characteristics of sun plant, and demanded many nutrients.4. Micropropagatin method was possible for the propagation of M.azedarach. but there were a difference for propagation rate among clones.5. A technique for producing new plants from the callus of R.pseudoaccasia was established.6. A technique for detecting the different of new plants was established by using protein analysis.7. Heredity varietions of new plants produced from salt treated callus were revealed by protein analysis.

苦楝是从九州地区的主要树种中选出的具有最高CO2固定能力的树种。从九州五个地区采集苦楝种子，并用于项目试验。通过RAPD分析揭示了这些种子的遗传变异，并通过田间遮荫和施肥试验揭示了这些种子的生长特性。介绍了苦楝的微繁殖方法。以刺槐为材料，进行了愈伤组织筛选和突变体产生的研究。通过这项研究，以下几点得到了明确。苦楝在不同地区或个体间存在着大量的遗传变异. RAPD分析表明，遗传距离与采集地的距离成正比，尤其是屋久岛和谷岛与其他地区的遗传距离差异较大.苦楝具有阳生植物的特性，对营养的要求较高.微繁技术是苦楝繁殖的可行方法。但无性系间的繁殖率存在差异.建立了从假红杜鹃愈伤组织诱导新植株的技术.建立了利用蛋白质分析技术检测新植物差异的技术.通过蛋白质分析，揭示了盐处理愈伤组织产生的新植株的遗传变异。

项目成果

KANETANI,S., WATANABE,A., SHIRAISI,S., GYOKUSEN,K., and SAITO,A.: "Study on genetic variation of Melia azedarch Linn.Pollulations in Kyushu area using RAPD Markers." Bull.Kyushu Univ.For. 76. 1-9 (1997)

KANETANI,S.、WATANABE,A.、SHIRAISI,S.、GYOKUSEN,K. 和 SAITO,A.：“利用 RAPD 标记研究苦楝的遗传变异。九州地区的种群。”

金 整一 ら: "Study on Genetic Variation of Melia azedarch Linn.Pollulations in Kyushu Area Using RAPD Markers." 九州大学農学部演習林報告. 76. 1-9 (1997)

Seiichi Kim 等人：“利用 RAPD 标记研究九州地区楝树的遗传变异。九州大学农学部实验森林报告”。

CHEN,R., GYOKUSEN,K., and SAITO,A.: "Screening of NaCl-tolerancet Robinia pseudoacacia L.callus through in Vitro culture" Bull.Kyushu Univ.For. 75. 15-26 (1996)

CHEN,R.、GYOKUSEN,K. 和 SAITO,A.：“通过体外培养筛选耐 NaCl 的刺槐愈伤组织”Bull.Kyushu Univ.For。

陳 任・玉泉 幸一郎・斉藤 明: "ニセアカシヤ(Robinia pseudoacacia L.)の耐塩性カルスの選抜" 九州大学農学部演習林報告. 75. 15-26 (1996)

陈仁，玉泉晃一郎，斋藤晃：“刺槐耐盐愈伤组织的选择”。九州大学农学部实验林报告。75. 15-26 (1996)

陳任: "ニセアカシア(Robinia pseudoacacia L.)の耐塩性カルスの選抜" 九州大学農学部演習林報告. 75. 15-26 (1996)

陈任：“刺槐耐盐愈伤组织的选择”。九州大学农学部实验林报告75。15-26（1996）。