The development of the treatment of autoimmune diseases by cytokine gene transfer

(1)髓鞘碱性蛋白（MBP）特异性T细胞系的建立；用MBP肽（68-88）免疫Lewis大鼠获得淋巴结细胞，用MBP和白细胞介素2重复刺激培养。将获得的T细胞系转移至幼稚同基因大鼠，证实其诱导脑脊髓炎（实验性过敏性脑脊髓炎；EAE）。(2)细胞因子基因向T细胞系的转移；制备细胞因子基因（IL-4、IL-10、tgf - β）表达载体，转染COS细胞。培养上清具有相应的细胞因子活性，转染tgfβ的COS细胞上清对mbp刺激的T细胞株增殖有明显抑制作用。将这些细胞系转移到经MBP肽免疫的Lewis大鼠体内，观察细胞因子基因转移是否能成功抑制EAE的诱导。

(1) Establishment of myelin basic protein (MBP)-specific T cell lines ;Lymph node cells were obtained from Lewis rats immunized with MBP peptide (68-88) and cultured with a repetitive stimulation with MBP and interleukin 2. T cell lines thus obtained were transfered to naive syngeneic rats and the induction of encephalomyelitis (experimental allergic encephalomyelitis ; EAE) was confirmed.(2) Transfer of cytokine genes to T cell lines ;Expression vectors for cytokine genes (IL-4, IL-10, TGFbeta) were prepared and transfected into COS cells. The culture supernatants were confirmed to have corresponding cytokine activities and those from TGFbeta-transfected COS cells strongly inhibited the proliferation of MBP-stimulated T cell lines in vitro. These cell lines were transferred into Lewis rats immunized with MBP peptide to examine whether cytokine-gene transfer successfully inhibit the induction of EAE.