The relation between the function of brain catecholamine neuron and cytokines

雄性Wistar大鼠被抑制4小时或每天4小时，连续2或3天。约束30 min后处死大鼠，解剖下丘脑和中脑。采用半定量逆转录聚合酶链式反应（RT-PCR）法检测白细胞介素(IL) -6受体(R) mRNA水平。在抑制4小时的大鼠中，这两个区域的IL-6R信息明显减少。抑制的重复通常会导致IL-6R信息的进一步减少。另外的实验表明，这种减少也可以由短期的限制引起，比如30分钟的限制，并且在限制之后持续几个小时以上。使用同样的方法，我们正在研究IL-6信息和各种神经递质受体信息的变化。在短期抑制的情况下，IL-6信息可能在短暂下降后增加。这些结果可能与IL-6的各种作用一致，如神经营养作用和下丘脑垂体肾上腺轴的激活。我们计划更详细地阐明脑细胞因子的作用。

Male Wistar rats were restrained for 4 hours or at 4 hours/day for 2 or 3 days. The rats were decapitated 30 minutes after the restraint and the hypothalamus and midbrain were dissected. The interleukin (IL) -6 receptor (R) mRNA levels were determined by a semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) method.The IL-6R message in the both regions was significantly decreased in the 4-hour restrained rats. The repetition of restraint generally induced a further decrease of the IL-6R message. Additional experiments revealed that such a reduction was also induced by the short-term, such as 30-minute restraint and lasted more than several hours after the restraint.Using the same method, we are studying changes of IL-6 message and the messages of various neurotransmitter receptors. In the case of short-term restraint, the IL-6 message could be increased after a transient decrease.These results may be consistent with various actions of IL-6, such as neurotrophic action and the activation of hypothalamopituitary adrenal axis. We plan to clarify the actions of brain cytokines in more detail.