The relation between the function of brain catecholamine neuron and cytokines

脑儿茶酚胺神经元功能与细胞因子的关系

• 批准号: 07457208
• 负责人: KOMORI Teruhisa
• 金额: $ 4.42万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457208/
• 关键词: brain cytokines; interleukin-6; interleukin-6 receptor; PCR; stress; neurotransmitter; カテコールアミソニューロン; サイトカイン

Male Wistar rats were restrained for 4 hours or at 4 hours/day for 2 or 3 days. The rats were decapitated 30 minutes after the restraint and the hypothalamus and midbrain were dissected. The interleukin (IL) -6 receptor (R) mRNA levels were determined by a semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) method.The IL-6R message in the both regions was significantly decreased in the 4-hour restrained rats. The repetition of restraint generally induced a further decrease of the IL-6R message. Additional experiments revealed that such a reduction was also induced by the short-term, such as 30-minute restraint and lasted more than several hours after the restraint.Using the same method, we are studying changes of IL-6 message and the messages of various neurotransmitter receptors. In the case of short-term restraint, the IL-6 message could be increased after a transient decrease.These results may be consistent with various actions of IL-6, such as neurotrophic action and the activation of hypothalamopituitary adrenal axis. We plan to clarify the actions of brain cytokines in more detail.

将雄性Wistar大鼠束缚4小时或以4小时/天束缚2或3天。束缚30分钟后将大鼠断头，解剖下丘脑和中脑。用半定量逆转录聚合酶链反应（RT-PCR）法检测大鼠海马两区白细胞介素（IL）-6受体（IL-6 R）mRNA水平，结果显示：束缚4 h后，两区IL-6 R mRNA表达均明显降低。重复的约束一般诱导IL-6 R信息的进一步减少。进一步的实验表明，这种减少也是由短期的，如30分钟的限制，并持续几个小时以上的限制后，使用相同的方法，我们正在研究IL-6的信息和各种神经递质受体的信息的变化。在短期束缚的情况下，IL-6的信息量在短暂下降后又增加，这可能与IL-6的多种作用有关，如神经营养作用和激活下丘脑-肾上腺皮质轴。我们计划更详细地阐明脑细胞因子的作用。

项目成果

Teruhisa Komori,et al.: "Reduced expression of interleukin-6 receptor mRNA in the hypothalamus of restrained rat" Human Psychopharmacology. (in press).

Teruhisa Komori 等人：“抑制大鼠下丘脑中白细胞介素 6 受体 mRNA 的表达减少”人类精神药理学。

Teruhisa Komori, et al.: "Reduced expression of interleukin-6 receptor mRNA in the hypothalamus of restrained rat" Human Psychopharmacology. (in press).

Teruhisa Komori 等人：“抑制大鼠下丘脑中白细胞介素 6 受体 mRNA 的表达减少”人类精神药理学。

Teruhisa Komori, et al.: "Reduced expression of interleukin-6 receptor mRNA in the hypothalamus of restrained rat." Human Psychopharmacology. (in press).

Teruhisa Komori 等人：“抑制大鼠下丘脑中白细胞介素 6 受体 mRNA 的表达减少。”