The relation between interleukin-6 and adrenergic and opioid receptor mRNA expressions in the brain ; the role of stress response

脑内白细胞介素6与肾上腺素受体和阿片受体mRNA表达的关系；

• 批准号: 10670895
• 负责人: KOMORI Teruhisa
• 金额: $ 2.05万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670895/
• 关键词: interleukin-6; interleukin-6 receptor; α1 adrenocepter; opioid receptor; brain; mRNA; PCR; stress; 脳内サイトカイン; アドレナリン受容体; RT-PCR; ラット; 脳内インターロイキン-6; 脳内アドレナリン受容体; 脳内オピオイド受容体; 脳内インターロイキン-6受容体

The reverse transcriptase-polymerase chain reaction was used. After rats had been restrained for 10-240 min, 30- and 60-min stress resulted in a significant fall in the level of α1 adrenoceptor mRNA in the hypothalamus, while significant elevation was noted after 60-, 120- and 240-min stress in the midbrain. When rats had been restrained for 4 h or 4 h per day for 2 or 3 consecutive days, 3-day stress resulted in a significant decrease in the level of α1 adrenoceptor mRNA, whereas single stress did not significantly affect it in the hypothalamus. In the midbrain, the level significantly increased after a single stress, but significantly decreased after repeated stress. The regional differences may be due to involvement of hypothalamic-pituitary-adrenal axis. The exact mechanism of the observed fall in the both regions after repeated stress is not clear and no previous studies have demonstrated such findings. On the expression of interleukin-6 (IL-6) and IL-6 receptor (IL-6R) mRNAs, single stress did not significantly affect the expression of IL-6 mRNA but significantly reduced the level of IL-6R mRNA, while 2- and 3- day stress significantly reduced the both levels in the hypothalamus. In the midbrain, single stress resulted in a significant rise in the level of IL-6 mRNA, and a significant fall in the level of IL-6R mRNA. Two day stress did not significantly affect the both levels but significantly reduced the both levels in the same region. IL-6 might be suppressed by hypothalamic-pituitary-adrenal axis. We cannot as yet explain the mechanism for observed findings on the repeated stress. We are investigating the effects of stress on the opioid receptor subtypes and the effects of agonists and antagonists of opioid and adrenergic receptor subtypes on IL-6 and IL-6R mRNA.

采用逆转录-聚合酶链反应。束缚10-240 min后，应激30和60 min可导致下丘脑α1肾上腺素能受体mRNA水平显著下降，而60、120和240 min可导致中脑α1肾上腺素能受体mRNA水平显著升高。当大鼠每天被束缚4 h或4 h，连续束缚2或3d后，3d的应激导致下丘脑α1肾上腺素能受体mRNA水平显著下降，而单一应激对下丘脑α1肾上腺素能受体mRNA水平无显著影响。在中脑中，单次应激后该水平显著升高，而重复应激后该水平显著降低。这种区域性差异可能与下丘脑-垂体-肾上腺轴的受累有关。重复压力后观察到的两个区域下降的确切机制尚不清楚，以前的研究也没有证明这样的发现。在白细胞介素6（IL-6）和IL-6受体（IL-6 R）mRNA的表达方面，单一应激对下丘脑IL-6 mRNA的表达无显著影响，但显著降低了IL-6 R mRNA的表达水平，而2 d和3 d应激则显著降低了下丘脑IL-6和IL-6 R mRNA的表达水平。在中脑，单一应激导致IL-6 mRNA水平显著升高，IL-6 R mRNA水平显著下降。两天的压力没有显着影响这两个水平，但显着降低了这两个水平在同一地区。IL-6可能受下丘脑-垂体-肾上腺轴的抑制。我们还不能解释在重复压力下观察到的结果的机制。我们正在研究应激对阿片受体亚型的影响以及阿片受体和肾上腺素能受体亚型的激动剂和拮抗剂对IL-6和IL-6 R mRNA的影响。

项目成果

Satoru Miyahara, et al.: "Effects of single and repeated stresses on the expression of mRNA for α1-adrenoceptors in the rat hypothalamus and midbrain"European Journal of Pharmacology. 379(1). 111-114 (1999)

Satoru Miyahara 等人：“单次和重复应激对大鼠下丘脑和中脑 α1-肾上腺素受体 mRNA 表达的影响”欧洲药理学杂志 379(1) (1999)。

Satoru Miyahara,et al.: "Effects of repeated stress on expression of interleukin(IL-6) and IL-6 receptor mRNAs in rat hypothalamus and midbrain"Life Science. 66・6. PL93-PL98 (2000)

Satoru Miyahara 等：“重复应激对大鼠下丘脑和中脑中白细胞介素 (IL-6) 和 IL-6 受体 mRNA 表达的影响”生命科学 66・6 (2000)。