The relation between interleukin-6 and adrenergic and opioid receptor mRNA expressions in the brain ; the role of stress response

采用逆转录-聚合酶链反应。大鼠应激10 ~ 240 min后，应激30、60 min下丘脑α1肾上腺素能受体mRNA水平显著下降，应激60、120、240 min中脑α1肾上腺素能受体mRNA水平显著升高。连续2、3天，每天4小时或每天4小时的应激可导致下丘脑α1肾上腺素能受体mRNA水平显著降低，而单一应激对下丘脑α1肾上腺素能受体mRNA水平无显著影响。在中脑，单次应激显著升高，多次应激显著降低。区域差异可能与下丘脑-垂体-肾上腺轴受累有关。在反复应激后观察到的两个区域下降的确切机制尚不清楚，以前也没有研究证明这样的发现。对白细胞介素-6 （IL-6）和IL-6受体（IL-6R） mRNA的表达，单次应激对下丘脑IL-6 mRNA表达无显著影响，但显著降低IL-6R mRNA表达水平，而2和3 d应激显著降低下丘脑IL-6和IL-6R mRNA表达水平。在中脑，单一应激导致IL-6 mRNA水平显著升高，IL-6R mRNA水平显著下降。在同一地区，两天应激对这两个水平没有显著影响，但显著降低了这两个水平。IL-6可能受到下丘脑-垂体-肾上腺轴的抑制。我们还不能解释重复应力所观察到的结果的机制。我们正在研究应激对阿片受体亚型的影响以及阿片受体和肾上腺素能受体亚型的激动剂和拮抗剂对IL-6和IL-6R mRNA的影响。

The reverse transcriptase-polymerase chain reaction was used. After rats had been restrained for 10-240 min, 30- and 60-min stress resulted in a significant fall in the level of α1 adrenoceptor mRNA in the hypothalamus, while significant elevation was noted after 60-, 120- and 240-min stress in the midbrain. When rats had been restrained for 4 h or 4 h per day for 2 or 3 consecutive days, 3-day stress resulted in a significant decrease in the level of α1 adrenoceptor mRNA, whereas single stress did not significantly affect it in the hypothalamus. In the midbrain, the level significantly increased after a single stress, but significantly decreased after repeated stress. The regional differences may be due to involvement of hypothalamic-pituitary-adrenal axis. The exact mechanism of the observed fall in the both regions after repeated stress is not clear and no previous studies have demonstrated such findings. On the expression of interleukin-6 (IL-6) and IL-6 receptor (IL-6R) mRNAs, single stress did not significantly affect the expression of IL-6 mRNA but significantly reduced the level of IL-6R mRNA, while 2- and 3- day stress significantly reduced the both levels in the hypothalamus. In the midbrain, single stress resulted in a significant rise in the level of IL-6 mRNA, and a significant fall in the level of IL-6R mRNA. Two day stress did not significantly affect the both levels but significantly reduced the both levels in the same region. IL-6 might be suppressed by hypothalamic-pituitary-adrenal axis. We cannot as yet explain the mechanism for observed findings on the repeated stress. We are investigating the effects of stress on the opioid receptor subtypes and the effects of agonists and antagonists of opioid and adrenergic receptor subtypes on IL-6 and IL-6R mRNA.