Basic research on cell-biological feature of multidrug resistant osteosarcoma and overcoming of resistance

DNA倍体分析结果显示，非二倍体(包括非整倍体和整倍体-多倍体)骨肉瘤对化疗的敏感性高于二倍体骨肉瘤。阿霉素结合研究表明，阿霉素结合能力与骨肉瘤对化疗的组织学反应密切相关。本研究建立的多重耐药小鼠骨肉瘤细胞不仅对阿霉素耐药，而且对长春新碱、博莱霉素、丝裂霉素等药物也耐药，该细胞系可用于克服耐药性的实验研究。在克服耐药性的各种方法中，维拉帕米、环孢菌素A、MS 209、吐温20或80、电子磁场、放射治疗对小鼠骨肉瘤有效。然而，临床上可能有电子磁场和MS 209可用，因为其中两种对人体无毒。

Result of DNA ploidy analysis in the study revealed that non-diploid (including aneuploid and euploid-polyploid) osteosarcoma was more sensitive to chemotherapy than diploid one. Adriamycin binding study showed that adriamycin binding ability was closely correlated with histological response to chemotherapy in human osteosarcomas. Multigrug resistant mouse osteosarcoma cells which was established in the study was resistant not only adriamycine also vincristin, bleomycine, mitomycine etc. This cell line was found to be useful for experimental study on overcoming of resistance. In various overcoming methods for resistance, verapamil, cyclosporin A,MS 209, Tween 20 or 80, electoron magnetic field, radiation were useful in mouse osteosarcoma. However, clinically electron magnetic field and MS 209 may available, because two of these were not toxic for human body.