Establishment of gene diagnosis and therapy for Endometrial carcinoma.

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1. We made various fragments derived from a human chromosome 1 by high dose radiation, and transferred them into endometrial carcinoma cells via microcell fusion. The introduction of a whole chromosome 1 resulted in the morphological alteration followed by cell senescence. In addition, telomerase activity was also suppressed in the microcell hybrids containing a chromosome 1. Evaluation of cell morphology in addition to telomerase activity in the microcell hybrid clones containing various fragments form chromosome 1 disclosed that 1q11-q21 or q31-ter region of chromosome 1 was critical for the suppression of endometrial cell growth properties.2. We established the reconstituted cells expressing that mutant K-Ras protein constitutively. Enchanced expression of ER protein that has a function as a transcription factor was shown in this cell. We also established the reconstituted cells expressing both the wild type of K-Ras protein and ER protein. Long term culture in the presence of 10% calf serum transformed the cell that was analogous to the level in the mutant K-Ras expressing cells, was shown in this transformed cells. These implicate ER protein in Ras-mediated transformation.