STUDY ON TRANSCRIPTION FACTORS REGULATING GENE EXPRESSION IN SEA URCHIN EMBRYOS

海胆胚胎基因表达调控转录因子的研究

• 批准号: 07458195
• 负责人: SHIMADA Hiraku
• 金额: $ 5.12万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07458195/
• 关键词: sea urchin; arylsulfatase; gene expression; embryo; enhancer; 0tx; insulator; 発生; 転写因子; ミトコンドリア; リボソームRNA; Ars遺伝子; 転写調節; Gストリング

The present research project has been undertaken to elucidate a cascade of transcription factors related to temporally and spatially regulated expression of genes during early development of sea urchin. Results obtained during the research period for this grant are as follows.(1) A procedure to introduce exogenous DNA into sea urchin eggs by a particle gun has been developed.(2) Temporal expression of the Ars gene is regulated by two distinct cisactive elements in its promoter region (from -252 to +38bp), though level of transcription initiated by these elements alone is very low and declines rapidly.(3) GGATTA sequences (known as binding sites for transcription factor 0tx) in the first intron of the Ars gene act as a transcriptional enhancer of this gene. Two distinct cDNAs (Hp0tx_E and Hp0tx_L) encoding Hp0tx have been cloned. Hp0tx_E is a maternal factor and suppresses the Ars gene transcription, while Hp0tx_L is produced in hatched blastulae and activates the Ars gene transcription. Two cDNAs are produced from the same gene by alternative splicing.(4) It is found that the region from -2686 to -2113bp of the Ars gene acts as an transcriptional insulator that prevents the Ars gene to be transcribed under the influence of promoter of the gene located in a closely vicinity.

本研究项目是为了阐明与海胆早期发育期间的时间和空间调节基因表达相关的一系列转录因子。 （1）已经开发了通过粒子枪引入外源性DNA到海胆卵中的一种程序。 （被称为转录因子0TX的结合位点）在ARS基因的第一个内含子中，作为该基因的转录增强子。已经克隆了两个编码HP0TX的两个不同的cDNA（HP0TX_E和HP0TX_L）。 HP0TX_E是母体因子，并抑制ARS基因转录，而HP0TX_L则在孵化的胚胎中产生并激活ARS基因转录。 （4）发现ARS基因的-2686至-2113bp的区域从同一基因产生了两个cDNA。

项目成果

N. Sakamoto et al: "Atriplex DNA configuration of the polypyrimidine: polypurine stretch in the 5′ flanking region of the sea urchin arylsulfatase gene." Zoological Science. 13. 105-109 (1996)

N. Sakamoto 等人：“聚嘧啶的 Atriplex DNA 构型：海胆芳基硫酸酯酶基因 5 侧翼区域的聚嘌呤延伸。动物学科学”13. 105-109 (1996)。

J.Morokuma, K.Akasaka, K.Mitsunaga-Nakatsubo and H.Shimada: "A cis-regulatory element within the 5' flanking region of arylsulfatase gene of sea urchin, Hemicentrotus pulcherrimus." Develop.Growth & Differ. 39(in press). (1997)

J.Morokuma、K.Akasaka、K.Mitsunaga-Nakatsubo 和 H.Shimada：“海胆 Hemicentrotus pulcherrimus 芳基硫酸酯酶基因 5 侧翼区域内的顺式调控元件。”

Harada et al: "Spatial expression of a forkhead homologue in the sea urchin embryo." Mechanism of Development. 60. 163-173 (1996)

Harada 等人：“叉头同源物在海胆胚胎中的空间表达。”

N.Sakamoto, K.Akasaka, T.Yamamoto and H.Shimada: "A triplex DNA configuration of the polypyrimidine : polypurine stretch in the 5' flanking region of the sea urchin arylsulfatase gene." Zool.Sci.13. 105-109 (1996)

N.Sakamoto、K.Akasaka、T.Yamamoto 和 H.Shimada：“聚嘧啶的三链 DNA 构型：海胆芳基硫酸酯酶基因 5 侧翼区域的聚嘌呤延伸。”

K. Yamasu et al: "Molecular cloning of a cDNA that encodes the precursor to several gastrula-inducing peptides, EGF-related peptides of the sea urchin" European Journal of Biochemistry. 228. 515-523 (1995)

K. Yamasu 等人：“编码几种原肠胚诱导肽（海胆 EGF 相关肽）前体的 cDNA 的分子克隆”《欧洲生物化学杂志》。