Regulation of gene expression during development

发育过程中基因表达的调控

基本信息

批准号：
04304007
负责人：
SHIMADA Hiraku
金额：
$ 12.16万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

项目摘要

A recent remarkable accumulation of information on gene regulation of various events during early development of animals has been obtained through studies using only limitted variety of animal species, such as some species of mammals, amphibians, an insect (Drosophila) and a nematoda (C.elegans). However, for an extensive understanding of molecular processes governing early development, the detailed information on the regulation of gene expression during embryogenesis of a large variety of animals is definitely expected. The present research project has thus been proposed to access the comparative aspect of gene regulation of early development. The following studies were performed in this project. (1) The collagenase gene was cloned from the frog (Rana catesbiana) and its expression in larval skin was studied in relation to metamorphosis. (2) A cDNA,expression of which changes markedly during metamorphosis, was cloned from liver of Xenopus laevis tadpole. (3) A trial to detect sperm-specific genes from spermatogonia of a newt (Triturus pyrrhogaster). (4)Genes for the fish (Oryzias latipes) hatching enzyme were cloned and their expression in prehatching stages was studied. (5) Transcription factors regulating the skeletal muscle actin genes in embryos of a solitary ascidian (Halocynthia roretzi) were studied. (6) LIM-class homeotic genes were cloned from Halocynthia roretzi and pattern of their expression during development was studied. (7) Genes related to budding of a colonial ascidian (Polyandrocarpa misakiensis) were cloned. (8) Regulation of genes for arylsulfatase in the sea urchin (Hemicentrotus pulcherrimus) and for the exogastrula-inducing peptides of the sea urchin (Anthocidaris crassispina) were studied. (8) Cloning of the treharase cDNA was started in relation to crustacian dormancy (Artemia salina). (9) An extensive search for genes related to regulation of planarian (Dugesia japonica) regeneration was started.

通过仅使用限制各种动物物种的研究，例如一些哺乳动物，两栖动物，昆虫（果蝇）和nematoda（C.Elegans），获得了动物早期发育期间各种事件的基因调节信息的显着积累。但是，为了广泛理解有关早期发育的分子过程，肯定会预计在各种动物的胚胎发生过程中，有关基因表达调节的详细信息。因此，已经提出了本研究项目，以获取早期发展基因调节的比较方面。在该项目中进行了以下研究。（1）从青蛙（Rana Catesbiana）克隆胶原酶基因，并研究了其在幼虫皮中的表达与变形有关。（2）从爪蟾tadpole的肝脏克隆了一种cDNA，其在变形过程中的表达发生了明显变化。（3）一项试验，以检测Newt的精子（Triturus pyrrhogaster）的精子基因。（4）将鱼（oryzias latipes）孵化酶的基因克隆，并研究了它们在预次匹配阶段的表达。（5）研究了调节孤立海豚（Halocynthia roretzi）胚胎中骨骼肌肌动蛋白基因的转录因子。（6）研究了lim-class同源基因的基因，并研究了其在发育过程中表达的模式。（7）与殖民时期的海鞘（polyandrocarpa misakiensis）有关的基因被克隆。（8）研究了研究海胆（Anthocidaris crassispina）的海胆（半肠曲霉）中芳基硫酸酯酶的基因和诱导外侧术诱导的肽的调节。（8）与甲壳虫休眠（Artemia salina）相关的三酶cDNA的克隆。（9）开始对与Planarian（Dugesia Japonica）再生有关的基因进行广泛的搜索。