Regulation of gene expression during development

发育过程中基因表达的调控

• 批准号: 04304007
• 负责人: SHIMADA Hiraku
• 金额: $ 12.16万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04304007/
• 关键词: gene expression; developmet; amphibia; fish; ascidia; sea urchin; artemia; planaria; 発生; 遺伝子クローニング; 遺伝子発現調節; 分化マーカー; 多様性

A recent remarkable accumulation of information on gene regulation of various events during early development of animals has been obtained through studies using only limitted variety of animal species, such as some species of mammals, amphibians, an insect (Drosophila) and a nematoda (C.elegans). However, for an extensive understanding of molecular processes governing early development, the detailed information on the regulation of gene expression during embryogenesis of a large variety of animals is definitely expected. The present research project has thus been proposed to access the comparative aspect of gene regulation of early development. The following studies were performed in this project. (1) The collagenase gene was cloned from the frog (Rana catesbiana) and its expression in larval skin was studied in relation to metamorphosis. (2) A cDNA,expression of which changes markedly during metamorphosis, was cloned from liver of Xenopus laevis tadpole. (3) A trial to detect sperm-specific genes from spermatogonia of a newt (Triturus pyrrhogaster). (4)Genes for the fish (Oryzias latipes) hatching enzyme were cloned and their expression in prehatching stages was studied. (5) Transcription factors regulating the skeletal muscle actin genes in embryos of a solitary ascidian (Halocynthia roretzi) were studied. (6) LIM-class homeotic genes were cloned from Halocynthia roretzi and pattern of their expression during development was studied. (7) Genes related to budding of a colonial ascidian (Polyandrocarpa misakiensis) were cloned. (8) Regulation of genes for arylsulfatase in the sea urchin (Hemicentrotus pulcherrimus) and for the exogastrula-inducing peptides of the sea urchin (Anthocidaris crassispina) were studied. (8) Cloning of the treharase cDNA was started in relation to crustacian dormancy (Artemia salina). (9) An extensive search for genes related to regulation of planarian (Dugesia japonica) regeneration was started.

最近，通过只使用有限种类的动物物种，如哺乳动物、两栖动物、昆虫(果蝇)和线虫(线虫)，获得了关于动物早期发育过程中各种事件的基因调控的显著积累的信息。然而，为了更广泛地了解控制早期发育的分子过程，关于大量动物胚胎发生过程中基因表达调控的详细信息是肯定值得期待的。因此，本研究项目被提议进入早期发育的基因调控的比较方面。本项目开展了以下研究工作。(1)从中国林蛙(Rana Catesbiana)中克隆了胶原酶基因，并研究了其在幼体皮肤中的表达与变态的关系。(2)从非洲爪哇蝌蚪肝脏中克隆了一个在变态过程中表达显著变化的基因。(3)利用金枪鱼精原细胞检测精子特异性基因的实验。(4)克隆了水稻孵化酶基因，并对其在孵化前的表达进行了研究。(5)对罗氏梭子蟹(Halocynthia Roretzi)胚胎中骨骼肌肌动蛋白基因的转录调控因子进行了研究。(6)克隆了罗氏梭梭LIM类同源异源基因，并对其在发育过程中的表达模式进行了研究。(7)克隆了群体海鞘(Polyandrocarpa Misakiensis)萌发相关基因。(8)研究了海胆的芳基硫酸酯酶基因和海胆的外源原肠诱导肽基因的调控。(8)克隆了盐生卤虫(Artemia Salina)甲壳休眠相关的海藻糖酶基因。(9)开始广泛寻找与涡虫(Dugesia Japan Onica)再生调控相关的基因。

项目成果

N.Ariyoshi: "cDNA cloning and expression of Xenopus sperm-specific basic nuclear protein 5 (SP5) gene." Molec.Reprod.Develop.37. 363-369 (1994)

N.Ariyoshi：“非洲爪蟾精子特异性碱性核蛋白 5 (SP5) 基因的 cDNA 克隆和表达。”

S.Yasumasu: "cDNAs and the genes of HCE and LCE,two constituents of the medaka hatching enzyme." Develop.Growth & Differ.36. 241-250 (1994)

S.Yasumasu：“cDNA 以及 HCE 和 LCE 基因，青鳉孵化酶的两种成分。”

K.Yoshizato: "Epidermal cells of the tail of anuran larva have a potency to transform into the adult type cells." Zool.Sci.10. 183-187 (1992)

K.Yoshizato：“无尾幼虫尾部的表皮细胞具有转变为成虫型细胞的能力。”

Ch.Katagiri: "Remodeling of sperm chromatin induced in egg extracts of amphibians." Int.J.Dev.Biol.38. 209-216 (1994)

Ch.Katagiri：“两栖动物卵提取物中诱导的精子染色质重塑。”

K.Yamada: "cis-Elements and protein factors related to regulation of transcription of arylsulfatase gene during sea urchin development." Develop.Growth & Differ.35. 703-710 (1993)

K.Yamada：“与海胆发育过程中芳基硫酸酯酶基因转录调节相关的顺式元件和蛋白质因子。”