Study on Molecular mechanisms regulating S phase in sea urchin embryo at cleavage stage.

海胆胚胎卵裂期S期调控的分子机制研究

基本信息

项目摘要

In most oviporous animals, the S phases during cleavage stage are extremely short, and little, if any, gap phases are detectable in the cell cycles at this stage of development. This project has been planned to elucidate the mechanism specifically regulating the S phases in the cells of cleavage embryos of sea urchin.(A) Role of deadenosine tetraphosphate (AP4A) on the regulation of s phase initiation. We have previously reported that the level of the soluble AP4A in sea urchin embryos at cleavage stage fluctuates cyclically during the cell cycle, drastically decreasing at the beginnning of S phase and increasing again during the S phase. We found that the decrease in the AP4A level is not due to enzymatic hydrolysis of this substance. A part of the free AP4A becomes bound to the nuclear matrix proteins at the beginning of S phase. The AP4A-binding activity of nuclear matrix of sea urchin embryos is most prominent at the beginning of S phase and become lowest during the other period of … More cell cycle. Gel electrophoretic analysis of the AP4A-binding proteins from nuclear matrix revealed the presence of two distince proteins, 23 kda protein and 70 kda protein. It seems that the 70 kda protein corresponds to nuclear lamins. We are now trying to purify the AP4A-binding protein of 23 kda.(B) Regulation of DNA replication at cleavage stage. It is generally believed that the extremely short S phases in cleavage-stage embryos of sea urchin are supported byactivation of many extra replication origins on genomic DNA. In the cells of abult sea urchin which replicate their DNA relatively slowly, these extra origins would be in an inactive state. To confirm this hypothesis, it is needed to compare the numbers of active origins on the DNA fragmenst with known length and nucleotide sequnce which are obtained from various stages of development. For this purpose, we have isolated the arylsulfatase gene (approx. 20 kbp) from sea urchin embryos by the conventional gene-cloning procedure starting from purification of this enzyme. Less
在大多数卵孔动物中,卵裂期的S相非常短,在这个发育阶段的细胞周期中几乎没有间隙相。本项目旨在阐明海胆卵裂胚胎细胞中S相的特异性调控机制。(A)去甲肾上腺素在S相启动中的调控作用。我们以前报道过卵裂期海胆胚胎中可溶性AP4A的水平在细胞周期中呈周期性波动,在S期开始急剧下降,在S期再次上升。我们发现,AP4A水平的下降并不是由于这种物质的酶水解。游离的AP4A的一部分在S期开始时与核基质蛋白结合。海胆胚胎核基质的AP4A结合活性在S期初最显著,在…的其他时期最低更多的细胞周期。对核基质中AP4A结合蛋白的凝胶电泳分析表明,存在两种差异蛋白:23kda蛋白和70kda蛋白。70KDA蛋白似乎与核层蛋白相对应。我们现在正在努力纯化23KDA的AP4A结合蛋白。(B)卵裂期DNA复制的调节。一般认为,海胆卵裂期胚胎极短的S期是由基因组DNA上许多额外复制起点的激活所支持的。在复制DNA相对较慢的成年海胆细胞中,这些额外的来源将处于不活跃状态。为了证实这一假设,需要比较从不同发育阶段获得的已知长度和核苷酸序列的DNA片段上的活性起始点的数量。为此,我们分离了芳基硫酸酯酶基因(约.从这种酶的纯化开始,通过常规的基因克隆程序从海胆胚胎中分离出20kbp的DNA。较少

项目成果

期刊论文数量(28)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Hiroshi,Sasaki: Comparative Biochemistry and Physiology. 88B. 147-152 (1987)
佐佐木浩:比较生物化学和生理学。
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    0
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Mizue, Morioka; H. Shimada: "AP4A-hydrolyzing activity in sea urchin embryos." Experimental Cell Research. 169. 57-62 (1987)
水江,盛冈;
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Hiraku, Shimada: "DNA replication and its regulation in cleavage embryos of sea urchin." Development, Growth and Differentiation. 29. 417-425 (1987)
Hiraku, Shimada:“海胆卵裂胚胎中的 DNA 复制及其调控。”
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    0
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Hiraku,Shimada: Development,Growth and Differentiation. 29. 417-425 (1987)
Hiraku、Shimada:发展、成长和差异化。
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SHIMADA Hiraku其他文献

SHIMADA Hiraku的其他文献

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{{ truncateString('SHIMADA Hiraku', 18)}}的其他基金

Joint Study on Gene Expression in Early Einbryo
早期胚胎基因表达联合研究
  • 批准号:
    09044226
  • 财政年份:
    1997
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C).
Cascade of transcription factors in sea urchin early embryos
海胆早期胚胎中转录因子的级联
  • 批准号:
    09480205
  • 财政年份:
    1997
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of systems for cell culture and expression vector to generate transgenic marine invertegra
开发细胞培养和表达载体系统以产生转基因海洋无脊椎动物
  • 批准号:
    08558080
  • 财政年份:
    1996
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
STUDY ON TRANSCRIPTION FACTORS REGULATING GENE EXPRESSION IN SEA URCHIN EMBRYOS
海胆胚胎基因表达调控转录因子的研究
  • 批准号:
    07458195
  • 财政年份:
    1995
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Requlation of gene expression in sea urchin embryo
海胆胚胎基因表达调控
  • 批准号:
    05454653
  • 财政年份:
    1993
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Regulation of gene expression during development
发育过程中基因表达的调控
  • 批准号:
    04304007
  • 财政年份:
    1992
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for Co-operative Research (A)
A study on the regulation of gene expression in sea urchin embryo.
海胆胚胎基因表达调控的研究。
  • 批准号:
    02454022
  • 财政年份:
    1990
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

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    2019
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    66.0 万元
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相似海外基金

Enhancing the cardioprotective effect of diadenosine tetraphosphate: designing inhibitors against Ap4A hydrolase
增强四磷酸二腺苷的心脏保护作用:设计 Ap4A 水解酶抑制剂
  • 批准号:
    nhmrc : 251679
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    2003
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    NHMRC Project Grants
Ap4A as an Alarmone in Stress Physiology
Ap4A 作为应激生理学中的警报素
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    6556864
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    2003
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    $ 4.16万
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Determination of the three-dimensional structure and substrate-binding mechanism of the assymetric Ap4A hydrol
不对称Ap4A水解物三维结构及底物结合机制的测定
  • 批准号:
    nhmrc : 7016
  • 财政年份:
    2000
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    $ 4.16万
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    Early Career Fellowships
Characterization of Ap4A Induced Release of Nitric Oxide From Endothelial Cells
Ap4A 诱导内皮细胞释放一氧化氮的表征
  • 批准号:
    9816681
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    1999
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    $ 4.16万
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    Continuing Grant
AP4A MODULATION PC12 CELL MONOAMINE OXIDASE ACTIVITY
AP4A 调节 PC12 细胞单胺氧化酶活性
  • 批准号:
    2274611
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    1996
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    $ 4.16万
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AP4A IN CELL PROLIFERATION, DNA REPLICATION AND REPAIR
AP4A 在细胞增殖、DNA 复制和修复中的作用
  • 批准号:
    3305890
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AP4A IN CELL PROLIFERATION, DNA REPLICATION AND REPAIR
AP4A 在细胞增殖、DNA 复制和修复中的作用
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    2183940
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AP4A IN CELL PROLIFERATION, DNA REPLICATION AND REPAIR
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AP4A IN CELL PROLIFERATION, DNA REPLICATION AND REPAIR
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THE CYTOLOGICAL AND PHYSIOLOGICAL MODE OF AP4A ACTION
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