Joint Study on Gene Expression in Early Einbryo

早期胚胎基因表达联合研究

• 批准号: 09044226
• 负责人: SHIMADA Hiraku
• 金额: $ 1.86万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C).
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09044226/
• 关键词: Transcription; Cis-element; Ets; ウニ胚; 骨マトリクスタンパク質; SM30; 一次間充組織細胞; 骨マトリックスタンパク質; 一次間充織細胞

In this research project in cooperation with Dr. Fred Wilt of the California University, we performed a series of study to elucidate a gene cascade regulating morphogenesis of sea urchin embryos, and obtained following results. 1. Expression of HpEts gene that regulated expression of genes encoding spicule matrix proteins is activated at the blastula stage. HpEts contains T domain in its 5' UTR. 2. cDNA of HpTbr, a sea urchin homolog of Tbr, was cloned. HpTbr cDNA becomes first detectable in pre-hatching blastulae and disappears after mesenchyme blastulae. Site of its expression is restricted to primary mesenchyme cells that specifically expresses HpEts, suggesting that HpTbr is related to regulation of transcription of HpEts gene. 3. HpLim, a sea urchin homolog of XLim, is transiently expressed in the vegetal half of the blastulae, and the embryos that express HpLim throughout the whole body by introduction of a large amount of HpLim mRNA in to eggs failed to form neither endoderm and mesoderm nor the oral and aboral axis. This suggests that HpLim is related to a vegetal signalling system needed for dorsoventral axis formation.

在与加利福尼亚大学的弗雷德·威尔特（Fred Wilt）博士合作的研究项目中，我们进行了一系列研究，以阐明调节海胆胚胎形态发生的基因级联，并获得了以下结果。 1。在囊泡阶段激活了调节编码Spicule基质蛋白的基因表达的HPET基因的表达。 HPET在其5'UTR中包含T域。 2。hptbr的cDNA是TBR的海胆同源物，被克隆。 HPTBR cDNA首先在预圈囊肿中可检测，并在间充质囊肿后消失。其表达的位点仅限于特异性表达HPET的原代间充质细胞，这表明HPTBR与HPET基因转录的调节有关。 3。Xlim的海胆同源物Hplim在胚芽的植物半时瞬时表达，并且通过引入大量的Hplim mRNA在整个身体中表达HPLIM的胚胎未能形成内胚层和中胚层和中胚层，也不形成中胚层，也没有形成口腔和口腔和异常轴。这表明HPLIM与背腹轴形成所需的植物信号系统有关。

项目成果

Y.Harada: "Spatial expression of a fork head homologue in the sea urchin embryo"Mech.Dev.. 60. 163-173 (1997)

Y.Harada：“海胆胚胎中叉头同源物的空间表达”Mech.Dev.. 60. 163-173 (1997)

Koji Akasaka, Hiroko Uemoto, Fred Wilt. Keiko Mitsunaga-Nakatsubo & Hiraku Shimada: "Oral-aboral ectoderm differentiation of the sea urchin embryos is disrupted in response to calcium ionophore"Dev. Growth and Differ.. 39. 373-379 (1997)

赤坂浩二、上本博子、弗莱德·威尔特。

K.Yamasu: "The functional organization of DNA elements regulating SM30a,a spicule matrix gene of sea unchin embryos"Dev.Growth & Difter.. 41. 81-91 (1999)

K.Yamasu：“调节海鳗胚胎的针状基质基因 SM30a 的 DNA 元件的功能组织”Dev.Growth

K.Akasaka: "Oral-cboral ectoderm differentiation of the sea urchin embryos is disrupted in response to calcium inophore"Dev.Growth & Difter.. 39. 373-379 (1997)

K.Akasaka：“海胆胚胎的口腔外胚层分化因钙载体而被破坏”Dev.Growth

K.Akasaka: "Upstream element of the sea urchin arylsulfatase gene serves as an insulator"Cell.Mol.Biol.. 45. 555-565 (1999)

K.Akasaka：“海胆芳基硫酸酯酶基因的上游元件充当绝缘体”Cell.Mol.Biol.. 45. 555-565 (1999)