Joint Study on Gene Expression in Early Einbryo

早期胚胎基因表达联合研究

• 批准号: 09044226
• 负责人: SHIMADA Hiraku
• 金额: $ 1.86万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C).
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09044226/
• 关键词: Transcription; Cis-element; Ets; ウニ胚; 骨マトリクスタンパク質; SM30; 一次間充組織細胞; 骨マトリックスタンパク質; 一次間充織細胞

In this research project in cooperation with Dr. Fred Wilt of the California University, we performed a series of study to elucidate a gene cascade regulating morphogenesis of sea urchin embryos, and obtained following results. 1. Expression of HpEts gene that regulated expression of genes encoding spicule matrix proteins is activated at the blastula stage. HpEts contains T domain in its 5' UTR. 2. cDNA of HpTbr, a sea urchin homolog of Tbr, was cloned. HpTbr cDNA becomes first detectable in pre-hatching blastulae and disappears after mesenchyme blastulae. Site of its expression is restricted to primary mesenchyme cells that specifically expresses HpEts, suggesting that HpTbr is related to regulation of transcription of HpEts gene. 3. HpLim, a sea urchin homolog of XLim, is transiently expressed in the vegetal half of the blastulae, and the embryos that express HpLim throughout the whole body by introduction of a large amount of HpLim mRNA in to eggs failed to form neither endoderm and mesoderm nor the oral and aboral axis. This suggests that HpLim is related to a vegetal signalling system needed for dorsoventral axis formation.

在本研究项目中，我们与加州大学的Fred Wilt博士合作，进行了一系列研究，以阐明调控海胆胚胎形态发生的基因级联，并获得了以下结果。1.调控骨针基质蛋白编码基因表达的HpEts基因的表达在囊胚期被激活。HpEts在其5'UTR中含有T结构域。2.克隆了Tbr的海胆同源物HpTbr的cDNA。HpTbr cDNA在孵化前囊胚中首先检测到，在间充质囊胚后消失。HpTbr的表达位点仅限于特异性表达HpEts的原代间充质细胞，提示HpTbr与HpEts基因的转录调控有关。3. HpLim是XLim的海胆同源物，在囊胚的植物性一半中瞬时表达，并且通过将大量HpLim mRNA引入卵中而在整个身体中表达HpLim的胚胎既不能形成内胚层和中胚层，也不能形成口轴和背口轴。这表明HpLim与背腹轴形成所需的植物信号系统有关。

项目成果

Y.Harada: "Spatial expression of a fork head homologue in the sea urchin embryo"Mech.Dev.. 60. 163-173 (1997)

Y.Harada：“海胆胚胎中叉头同源物的空间表达”Mech.Dev.. 60. 163-173 (1997)

M.Ogawa: "Asymmetrical distribution of mitochondrial rRNA into small micromeres of sea urchin embryos"Zool.Sci.. 16. 445-451 (1999)

M.Okawa：“线粒体 rRNA 不对称分布到海胆胚胎的小微米中”Zool.Sci.. 16. 445-451 (1999)

K. Yamasu & F. H. Wilt: "The functional organization of DNA elements regulating SM30a, a spicule matrix gene of sea urchin embryos."Dev. Growth & Differ. 41. 81-91 (1999)

K·山须

Daisuke kurokawa: "Cloning of Cyclin E cDNA of the sea Urchin,Hemicentrotus pulcherrimus." Zoological Scienu. 14. 791-794 (1997)

Daisuke kurokawa：“海胆 Cyclin E cDNA 的克隆，Hemicentrotus pulcherrimus。”

K.Akasaka: "Upstream element of the sea urchin arylsulfatase gene serves as an insulator"Cell.Mol.Biol.. 45. 555-565 (1999)

K.Akasaka：“海胆芳基硫酸酯酶基因的上游元件充当绝缘体”Cell.Mol.Biol.. 45. 555-565 (1999)