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An experimental investigation of locomotive behavior using an automated monitor system in the mouse and the rat : Effects of aging and NMDA antagonist treatment

使用自动监测系统对小鼠和大鼠的运动行为进行实验研究：衰老和 NMDA 拮抗剂治疗的影响

基本信息

批准号：
07551002
负责人：
SEKIGUCHI Shigehisa
金额：
$ 2.82万
依托单位：
Shiga Univ.
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

In Exp.1, age-sex related differences of the C57BI/6J mouse were studied in an open-field behavior. Total quaantities of locomotion, rearing and defecation were all found to differ significally between male and female mice of the older ages. The absolute rate of habituati on and the frequency of face-washing were found also to differ significantly as a function of aging. The possibility of age-sex related differences of the open-field behavior in the system of neuroendocrine is discussed.In Exp.2, rats given NMDA antagonist (MK801) were used in the open-field for three days. Male and female rats were injected ipwith either low dose (0.05mg/kg), middle dose (0.10mg/kg), high dose (0.20mg/kg) or equivalent volume of saline before testing of Day 2. The animal was placed individually in the field and was videotaped for 10-min period on three successive days. On the hige dose of MK801 induced hyperactivity of male rats. However, the high does of MK801 impaired locomotion and exploratory activity in female rats. In the female rats, indeed, the middle and high doses produced mild ataxi and head weaving in addition to the activity deficites. These results, together with the finding from previous experiment used with mice, further suggest that open field behavior and exploratory activity require NMDA-dependent synaptic plasticity

在实验1中，在旷场行为中研究了C57 BI/6 J小鼠的年龄-性别相关差异。结果表明，老年雌、雄小鼠的运动、直立和排便的总数量均存在显著差异。习惯化的绝对速率和洗脸的频率也被发现作为年龄的函数而显著不同。实验二用NMDA受体拮抗剂MK 801处理大鼠，连续3天，观察其旷场行为的变化，并探讨了旷场行为在神经内分泌系统中存在年龄性别差异的可能性。在第2天试验前，雄性和雌性大鼠腹腔注射低剂量（0.05 mg/kg）、中剂量（0.10 mg/kg）、高剂量（0.20 mg/kg）或等体积的生理盐水。将动物单独放置在田间，并连续三天进行10分钟的录像。对大剂量MK 801诱发的雄性大鼠多动有明显的抑制作用。然而，高剂量的MK 801损害了雌性大鼠的运动和探索活动。事实上，在雌性大鼠中，中剂量和高剂量除了活动缺陷外，还产生了轻度共济失调和头部摆动。这些结果，以及以前小鼠实验的发现，进一步表明旷场行为和探索活动需要NMDA依赖的突触可塑性