Development of Evanescent Wave Light Scattering Microscope Instrument and Evaluation of Polymer Particle Dynamics and Interaction near Interface

通过将逝去波与动态光散射技术相结合，成功地构建了逝去波光散射显微镜(EVLSM)。利用该技术研究了分散聚苯胶粒与玻璃表面相互作用的动态和静态特性。除了对势垒本身的估计外，还清楚地、定量地估计了粒子与玻璃和重力之间的静电相互作用产生的势垒中粒子热振动的动态行为。随着盐浓度的增加，势能极小值变浅，同时也清楚地观察到，随着盐浓度的增加，井中颗粒的振动运动幅度变大，发生大振动的概率变大。进一步用EVLSM直接估计了带电脂质体颗粒与玻璃表面相互作用的势能分布，它可以测量颗粒与玻璃表面之间的距离作为时间间隔的函数，其量级小于INS。这种新的EVLSM技术也被应用于聚苯乙烯乳胶颗粒与经化学修饰的玻璃表面之间的体系。这种关于动力学的信息对于正确理解相互作用势是必不可少的。EVLSM方法被证明是一种非常强大的技术，不仅可以估计电势分布，而且还可以估计动态特性。

The construction of the evanescent wave light scattering microscope (EVLSM) proved successful by the use of a novel technique combining the evanescent wave and the dynamic light scattering techniques. The dynamic and static characteristics of a polystyrene latex particle in dispersion interacting with a glass surface were studied by this technique. The dynamic behavior of the thermal vibration of the particle in a potential well created by electrostatic interaction between the particle and glass and gravity was clearly and quantitatively estimated, in addition to the estimation of the potential profile itself. The potential minimum became shallower with increasing added salt concentration, It was also clearly observed that the vibrational motion of the particle in the well became large in amplitude and the probability of the occurrence of the large vibration became large with increasing salt concentration. The potential profile of interaction between a charged liposome particle and glass surface was futhermore estimated directly by EVLSM, which can measure the distance between the particle and surface as a function of time at intervals in the order of less than ins, This new EVLSM technique was as well applied to the system between polystyrene latex particle and chemically-modified glass surface. Such information on the dynamics is essential for the correct understanding of the interaction potential. The EVLSM method is shown to be a very powerful technique for the estimation of not only the potential profilebut also dynamic characteristics.