Development of Evanescent Wave Light Scattering Microscope Instrument and Evaluation of Polymer Particle Dynamics and Interaction near Interface

通过将倏逝波和动态光散射技术相结合，成功地构建了倏逝波光散射显微镜（EVLSM）。利用该技术研究了分散态聚苯乙烯乳胶粒子与玻璃表面相互作用的动态和静态特性。除了势分布本身的估计之外，还清楚地和定量地估计了由颗粒与玻璃之间的静电相互作用和重力产生的势阱中的颗粒的热振动的动力学行为。随着外加盐浓度的增加，电位极小值变浅，同时也清楚地观察到井中粒子的振动幅度变大，发生大振动的概率也随着盐浓度的增加而增大。用EVLSM技术直接测定了荷电脂质体颗粒与玻璃表面相互作用的电位分布，并将其应用于聚苯乙烯乳胶颗粒与化学修饰的玻璃表面体系。这种动力学信息对于正确理解相互作用势是必不可少的。EVLSM方法被证明是一个非常强大的技术，不仅潜在的profilebut也动态特性的估计。

The construction of the evanescent wave light scattering microscope (EVLSM) proved successful by the use of a novel technique combining the evanescent wave and the dynamic light scattering techniques. The dynamic and static characteristics of a polystyrene latex particle in dispersion interacting with a glass surface were studied by this technique. The dynamic behavior of the thermal vibration of the particle in a potential well created by electrostatic interaction between the particle and glass and gravity was clearly and quantitatively estimated, in addition to the estimation of the potential profile itself. The potential minimum became shallower with increasing added salt concentration, It was also clearly observed that the vibrational motion of the particle in the well became large in amplitude and the probability of the occurrence of the large vibration became large with increasing salt concentration. The potential profile of interaction between a charged liposome particle and glass surface was futhermore estimated directly by EVLSM, which can measure the distance between the particle and surface as a function of time at intervals in the order of less than ins, This new EVLSM technique was as well applied to the system between polystyrene latex particle and chemically-modified glass surface. Such information on the dynamics is essential for the correct understanding of the interaction potential. The EVLSM method is shown to be a very powerful technique for the estimation of not only the potential profilebut also dynamic characteristics.