Diagnostic imaging of infection and thrombus using radiolabeled pap tides developed by the genetic engineering.

使用基因工程开发的放射性标记乳潮进行感染和血栓的诊断成像。

• 批准号: 07557066
• 负责人: ENDO Keigo
• 金额: $ 4.16万
• 依托单位: Gunma Univeristy
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07557066/
• 关键词: genetic engineering; ovarian cancer; bone marrow; radionuclide; leukocytes; モノクローナル抗体; 炎症; 画像診断; シングルチェイン抗体

Single chain atibody consists of peptides, genetically analyzed from monoclonal antibody, and is reactive with the corresponding antigens. The advantages of single chain antibodies are (1) no immunogenecity to humans, (2) chemically produced, and (3) responding to the same antigen with the original monoclonal antibody.Single chain antibody was obatained from the murine antibody reactive with ovarian cancer antigen CA125, and iodinated with radioiodine-125. However I-125 labeled antibody was not stable and its binding to CA125 antigen was not confirmed. Binding of heavy chain and light chain remains to be studied.In contrast, chimeric antibody which reserves the antigen-binding sites of murine antibody was successfully labeled with technetium-99m and I-125.Chimeric antibody reactive with NCA-95 bindis to human leukocytes and colorectal cancer cells expressing carcinoembryonic antigen (CEA). After getting the permission of clinical use from the ethical committee, Tc-99m labeled was safely administered and was very useful in patients with a plastic anemia, myelofibrosis and leukemia. Tc-99m labeled chimeric antibody to NCA-95 would be also clinically useful for the localization of infectious sites and abscess.

单链抗体由单克隆抗体基因分析的多肽组成，并与相应的抗原反应。单链抗体的优点是：（1）对人无免疫原性，（2）化学合成，（3）与原单克隆抗体对同一抗原反应。然而，I-125标记的抗体不稳定，并且其与CA 125抗原的结合未被证实。与此相反，保留鼠源抗体抗原结合位点的嵌合抗体成功地被~（99）Tc ~（99）m和~（125）I标记，与NCA ~（95）反应的嵌合抗体可与表达癌胚抗原（CEA）的人白细胞和大肠癌细胞结合。在获得伦理委员会的临床使用许可后，Tc-99 m标记的药物安全使用，并且在再生障碍性贫血、骨髓纤维化和白血病患者中非常有用。Tc-99 m标记的NCA-95嵌合抗体在临床上也可用于感染部位和脓肿的定位。

项目成果

Oriuchi, N., Endo, K.: "Semiquantitative SPECT tumor uptake of technetium-99m-labeled anti-CEA monoclonal antibody in colorectal tumor." J. Nucl. Med.36. 679-683 (1995)

Oriuchi, N., Endo, K.：“结直肠肿瘤中锝-99m 标记的抗 CEA 单克隆抗体的半定量 SPECT 肿瘤摄取。”

Oriuchi, N., Endo, K.: "Independent thallium-201 accumulation and F-18-fluorodeoxygulcose metabolism in glioma." J. Nucl. Med.(in press.).

Oriuchi, N., Endo, K.：“神经胶质瘤中独立的铊 201 积累和 F-18-氟脱氧葡萄糖代谢。”

Kobayashi,H.: "Inflammation-seeking scintigraphy with radiolabeled biotinylated polyclonal IgG followed by the injection of avidin chase." Nuclear Medicine & Biology. 23. 29-32 (1996)

Kobayashi,H.：“使用放射性标记的生物素化多克隆 IgG 进行炎症追踪闪烁扫描，然后注射抗生物素蛋白。”

Kobayashi, H., Endo, K.: "Inflammation-seeking scintigraphy with radiolabeled biotinylated polyclonal IgG followed by thd injection of avidin chase." Nucl. Med. Biology. 23. 29-32 (1996)

Kobayashi, H., Endo, K.：“使用放射性标记的生物素化多克隆 IgG 进行炎症追踪闪烁扫描，然后注射抗生物素蛋白。”

Sakahara, H., Hosono, M., Kobayashi, H., Yao, Z., Saga, T., Yano, S., Endo, K., Mori, T., Konishi, J.: "Effect of circulating antigen on immunoscintigraphy of ovarian cancer patients using anti-CA125 monoclonal antibody." Jpn.J.Cancer Res. 87. 655-661 (19

Sakahara, H.、Hosono, M.、Kobayashi, H.、Yao, Z.、Saga, T.、Yano, S.、Endo, K.、Mori, T.、Konishi, J.：“循环抗原的影响