Molecular analysis of epithelial mesenchymal interaction during tooth formatio

将小鼠胚胎牙胚分离成上皮细胞和间充质细胞，并进行联合培养，以检测牙齿发育和形成过程中的基因表达。使用从这些组合培养物制备的RNA制备互补DNA文库。我们克隆了牙齿发育过程中表达的新基因。我们还分析了6kb釉原蛋白基因启动子片段。利用C1细胞RNA构建的cDNA文库，克隆出noggin的cDNA，原位杂交揭示了noggin在硬组织形成和发育过程中的新表达模式。这些分子在胚胎发育过程中发挥信号作用。

Embryonic mouse tooth germs were excised and were devided into epithelium and mesenchyme followed by combination cultures to examine gene expression during tooth development and formation. Complementary DNA library was made using RNAs prepared from these combinatorial cultures. We cloned novel genes expressed during tooth development. We also analyzed 6kb amelogenin gene promoter fragment. Using another cDNA library made from C1 cell RNA,we cloned out noggin cDNA.In situ hybridization revealed novel expression pattern of noggin during hard tissue formation and development. These molecules would play their role as signals during embryogenesis.