DNA methylation and histone modification regulate differentiationof skeletal stem cells

DNA甲基化和组蛋白修饰调节骨骼干细胞的分化

• 批准号: 22390344
• 负责人: NIFUJI Akira
• 金额: $ 12.23万
• 依托单位: Tsurumi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390344/
• 关键词: 細胞分化; 骨格組織; 骨格系細胞; ヒストン; メチル化

Chromatin structure is a critical determinant to regulate gene expression and it alters during fate decision or/and cell differentiation. Patterns of post-translational modification at histone tails as well as methylation patterns of cytosine residues at DNA affect chromatin structure. In this study, we examined histone modification during skeletal cell differentiation. We observed dynamic changes of expression of H3K9methyltransferases at H3K9 and localization of three H3K9 methylation patterns, me1, me2, and me3 , during skeletal development. It also suggests that nhibition of H3K9 me2 affects gene expression and subsequent cell differentiation.

染色质结构是调控基因表达的关键决定因素，它在决定命运或/和细胞分化过程中发生变化。组蛋白尾部的翻译后修饰模式以及DNA上胞嘧啶残基的甲基化模式影响染色质结构。在这项研究中，我们研究了骨骼细胞分化过程中组蛋白的修饰。我们观察了骨骼发育过程中H3K9甲基转移酶在H3K9上表达的动态变化以及三种H3K9甲基化模式ME1、ME2和ME3的定位。这也表明抑制H3K9me2影响基因表达和随后的细胞分化。

项目成果

Extracellular modulators regulate Bone Morphogenic Proteins in skeletal tissue

细胞外调节剂调节骨骼组织中的骨形态发生蛋白

• 发表时间: 2010
• 期刊: J Oral Biosciences
• 作者: Nifuji A;Ideno H;Takanabe R;Noda M
• 通讯作者: Noda M

Collagen Gel Culture Accelerates Proliferation of Mouse Periodontal Ligament Cells.

胶原凝胶培养加速小鼠牙周膜细胞的增殖。

• 发表时间: 2010
• 作者: Shimada A;Shibata T;Komatsu K;Nifuji A
• 通讯作者: Nifuji A

A Molecular mechanisms of skeletal tissue formation.

A 骨骼组织形成的分子机制。

• 发表时间: 2013
• 期刊: J Physical Fitness and Sports Medicine.
• 作者: Morita J;Funato N;Kobayashi Y;Nakamura M;and Moriyama K;Nifuji
• 通讯作者: Nifuji

Nifuji A.The myogenic conversion from fibroblastsinto myogenic cells is blocked by Trichostatin A through suppression of myogenin expression

Nifuji A.曲古抑菌素 A 通过抑制肌生成素表达来阻断从成纤维细胞到肌原细胞的肌原性转化

• 发表时间: 2011
• 作者: Ideno H;Araki R,Imaizumi K;Abe M
• 通讯作者: Abe M

Annexin a5 Expression Regulates Periodontal Tissue and Cementum Formation

膜联蛋白 a5 表达调节牙周组织和牙骨质形成

• 发表时间: 2011
• 作者: Akemi Shimada;Koichiro Komatsu;Kazuhisa Nakashima;Norio Amizuka;Bent Brachvogel;Akira Nifuji
• 通讯作者: Akira Nifuji