Establishment of the estimation method for analgesia using stress-induced hyperalgesic animals

1、汉方药猫布氏西伸藤口服100和300 mg/kg对反复冷应激（RCS）引起的大鼠和小鼠痛敏有镇痛作用，对健康大鼠无镇痛作用。吗啡（0.1-3 mg/kg，s.c.）和双氯芬酸（0.5-80 mg/kg，p.o.）2、猫不食Saishin-toh的抗伤害作用鞘内注射肾上腺素能神经毒素5，7-二羟色胺（5，7-DHT）可显著抑制RCS大鼠（300 mg·kg-1）的多巴胺能神经递质（300 mg·kg-1），而注射儿茶酚胺能神经毒素6-羟多巴胺（6-OHDA）则无此作用。猫布氏西辛妥的抗伤害作用鞘内注射5-羟色胺受体拮抗剂赛庚啶、甲硫替平和麦角新碱可显著抑制RCS大鼠的（300 mg/kg），但注射肾上腺素受体拮抗剂酚妥拉明则无此作用。4，经口给予100和300 mg·kg-1的汉方药物京石藤对RCS引起的大鼠痛敏有镇痛作用，而对健康大鼠无镇痛作用。鞘内注射6-OHDA可抑制京石藤的镇痛作用（300 mg/kg），但5，7-DHT对Keishi-toh的抗伤害作用无影响。6.如上所述，我们用RCS引起的痛敏动物检测了Maoh-Bushi-Saishin-toh和Keishi-toh的抗伤害作用。此外，这些研究结果表明，Maoh-Bushi-Saishin-toh和Keishi-toh的抗伤害作用可能至少部分介导的下行肾上腺素能和下行去甲肾上腺素能系统，分别。

1, The Kampo medicine Maoh-Bushi-Saishin -toh at peroral doses of 100 and 300 mg/kg produced an antinociception in rats and mice that showed hyperalgesia induced induced by repeated cold stress (RCS), without effect in healthy ones. Morphine (0.1-3 mg/kg, s.c.) and diclofenac (0.5-80 mg/kg, p.o.) also produced an antinociception in RCS animals.2, The antinociception of Maoh-Bushi-Saishin-toh (300 mg-kg, ) in the RCS rat was significantly suppressed by intrathecal pretreatment with the serotonergic neurotoxin 5,7-dihydroxytryptamine (5,7-DHT) but not with the catecholaminergic neurotoxin, 6-hydroxydopamine (6-OHDA).3, The antiociception of Maoh-Bushi-Saishin-toh (300 mg/kg) in the RCS rat was significantly suppressed by intrathecal injection with antagonists for serotonin receptors, cyproheptadine, methiotepine and methysergide, but not with an antagonist for adrenaline receptors, phentolamine.4, The Kampo medicine Keishi-toh at peroral doses of 100 and 300 mg-kg produced an antinociception in rats that showed hyperalgesia induced by RCS,without effect in healthy ones.5, The intrathecal pretreatment with 6-OHDA tended to suppress the antinociception of Keishi-toh (300 mg/kg) in the RCS rat, but 5,7-DHT had no effect on the antinociception of Keishi-toh.6, As mentioned above, we detected antinociceptive effects of Maoh-Bushi-Saishin-toh and Keishi-toh using RCS-induced hyperalgesic animals. In addition, these findings suggest that the antinociception of Maoh-Bushi-Saishin-toh and Keishi-toh may be at least in part mediated by the descending serotonergic and the descending noradrenergic systems, respectively.