Establishment of the estimation method for analgesia using stress-induced hyperalgesic animals

短句来源1、汉布药毛补-赛辛-土经口服100、300 mg/kg对重复性冷应激（RCS）致痛觉过敏的大鼠和小鼠均有抗痛觉作用，对健康小鼠无影响。吗啡（0.1-3 mg/kg, s.c）和双氯芬酸（0.5-80 mg/kg, p.o.）也对RCS动物产生抗痛觉作用。2、5 -羟色胺能神经毒素5,7-二羟色胺（5,7- dht）鞘内预处理能显著抑制毛-布希-塞辛-托（300 mg-kg,）对RCS大鼠的抗炎作用，而儿茶酚胺能神经毒素6-羟多巴胺（6-OHDA）鞘内预处理对毛-布希-塞辛-托（300 mg-kg,）的抗炎作用无显著抑制作用。3、鞘内注射5 -羟色胺受体拮抗剂、甲氧替平、甲氧替平和甲塞吉特能显著抑制毛-布希-赛辛-toh （300 mg/kg）对RCS大鼠的抗痛觉作用，而肾上腺素受体拮抗剂酚妥拉明对其无明显抑制作用。4、康布药Keishi-toh口服100和300 mg-kg对RCS致痛觉过敏大鼠有抗痛觉作用，对健康大鼠无影响。5、鞘内预处理6-OHDA （300 mg/kg）对RCS大鼠龟石土的抗伤性有抑制作用，而5,7- dht对龟石土的抗伤性无影响。6、如上所述，我们用rcs诱导的痛觉过敏动物检测了Maoh-Bushi-Saishin-toh和Keishi-toh的抗痛觉作用。此外，这些研究结果表明，Maoh-Bushi-Saishin-toh和Keishi-toh的抗痛觉作用可能至少部分由血清素能下降和去甲肾上腺素能下降系统介导。

1, The Kampo medicine Maoh-Bushi-Saishin -toh at peroral doses of 100 and 300 mg/kg produced an antinociception in rats and mice that showed hyperalgesia induced induced by repeated cold stress (RCS), without effect in healthy ones. Morphine (0.1-3 mg/kg, s.c.) and diclofenac (0.5-80 mg/kg, p.o.) also produced an antinociception in RCS animals.2, The antinociception of Maoh-Bushi-Saishin-toh (300 mg-kg, ) in the RCS rat was significantly suppressed by intrathecal pretreatment with the serotonergic neurotoxin 5,7-dihydroxytryptamine (5,7-DHT) but not with the catecholaminergic neurotoxin, 6-hydroxydopamine (6-OHDA).3, The antiociception of Maoh-Bushi-Saishin-toh (300 mg/kg) in the RCS rat was significantly suppressed by intrathecal injection with antagonists for serotonin receptors, cyproheptadine, methiotepine and methysergide, but not with an antagonist for adrenaline receptors, phentolamine.4, The Kampo medicine Keishi-toh at peroral doses of 100 and 300 mg-kg produced an antinociception in rats that showed hyperalgesia induced by RCS,without effect in healthy ones.5, The intrathecal pretreatment with 6-OHDA tended to suppress the antinociception of Keishi-toh (300 mg/kg) in the RCS rat, but 5,7-DHT had no effect on the antinociception of Keishi-toh.6, As mentioned above, we detected antinociceptive effects of Maoh-Bushi-Saishin-toh and Keishi-toh using RCS-induced hyperalgesic animals. In addition, these findings suggest that the antinociception of Maoh-Bushi-Saishin-toh and Keishi-toh may be at least in part mediated by the descending serotonergic and the descending noradrenergic systems, respectively.