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Development of a functional imaging system of the brain using dadual-wavelength measuremant and improved voltage sensitive dyes

使用双波长测量和改进的电压敏感染料开发大脑功能成像系统

基本信息

批准号：
07558097
负责人：
TANIFUJI Manabu
金额：
$ 13.57万
依托单位：
The institute of Physical and Chemical research (RIKEN) (1996-1997)University of Fukui (1995)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

To establish optical imaging technique to visualize spatio-temporal patterns of neural activities from cortex in alive animals, we investigated two aspects : (1) evaluation of voltage-sensitive dye related signal using a slice preparation, and (2) development of an imaging technique from a cortical surface. The former part includes designing a new voltage sensitive dye and the later part includes development of surgical techniques to keep exposed cortex for a long period of time.(1) Evaluation of voltage sensitive dye related signalFirst, we used visual cortical slices to examine relation between dyerelated signals and neural activities. Our main finding is that the dye signal mainly reflects synaptic potential but not spiking activities. This is an important aspect when we correlate the optical signal with conventional extracellular recordings in which spiking activities are measured. Second, we synthesized a new voltage sensitive dye having two chromophores, which is designed to enha … More nce the optical signal by the mechanism of energy transfer. We have started to test the dye properties. Thirdly, we applied the voltage sensitive dye measurement from brain slices to study neural circuitry and pharmacology in various regions in the brain.(2) Development of imaging technique from cortical surfaceTo visualize optical signals from cortical surface it is important to keep exposed surface in good condition. We develop several surgical techniques to do so, and evaluate the cortical condition by imaging intrinsic signal evoked by visual stimulation. As a result, we made it possible to record the intrinsic signal from the same cortical surface repeatedly from the same animal. By using this technique we found columnar organization in inferotemporal cortex in monkey.Our final goal of the project is to visualize dye related signals from cortical surface of alive animals. Although this final goal could not beachieved unfortunately, the present study provides important information to do so. Less

为了建立光学成像技术来可视化活体动物大脑皮质神经活动的时空模式，我们从两个方面进行了研究：(1)使用切片制备法评估与电压敏感的染料相关的信号；(2)开发基于皮质表面的成像技术。前半部分包括设计一种新的电压敏感染料，后半部分包括长时间保持大脑皮质暴露的外科技术的发展。(1)电压敏感染料相关信号的评估首先，我们使用视觉皮层切片来检测与染料相关的信号与神经活动之间的关系。我们的主要发现是，染料信号主要反映突触电位，而不是棘波活动。当我们将光信号与测量尖峰活动的传统细胞外记录相关联时，这是一个重要的方面。其次，我们合成了一种新的具有两个生色团的压敏染料，它是为增强…而设计的并通过能量传递机制实现了光信号的传输。我们已经开始测试染料的性能。第三，我们应用脑片的电压敏感染料测量来研究大脑不同区域的神经回路和药理学。(2)皮质表面成像技术的发展为了可视化来自皮质表面的光信号，保持暴露表面的良好状态是很重要的。我们开发了几种手术技术来做到这一点，并通过成像视觉刺激诱发的内在信号来评估皮质状况。因此，我们有可能重复记录来自同一动物的同一皮质表面的内在信号。通过使用这项技术，我们在猴子的颞下皮质中发现了柱状组织。我们项目的最终目标是可视化活动物皮质表面与染料相关的信号。虽然这一最终目标不幸未能实现，但本研究为实现这一目标提供了重要信息。较少