Optical imaging of synaptic transmission: a transformative technology to probe the functional determinants of behaviour.

突触传递的光学成像：一种探索行为功能决定因素的变革性技术。

• 批准号: 2886712
• 金额: --
• 依托单位: University of Sussex
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2886712
• 关键词: Optical; imaging; synaptic; transmission; transformative

First rotation- Optical imaging of synaptic transmission: a transformative technology to probe the functional determinants of behaviour.3D live imaging is key to understanding biological processes such as nervous system control of behaviour, but it is challenging to achieve at speed.One approach is selective plane illumination microscopy (SPIM) which uses a thin sheet of light imaged onto a high-speed camera: rapid z-movement of both light-sheet and imaging objective allows high-speed iobservation in 3D. We will develop a SPIM with improved resolution that uses two-photon Bessel beam excitation to image through the brain of a small live animal such as a larval zebrafish or C. elegans. This instrument, the first in the UK, will allow the student to develop a project based around visualizing the dynamics of signals transmitted at synaptic connections between neurons using the fluorescent glutamate reporter iGlUSnFR. This transformative technology will reveal how information flows through the brain as an animal interacts with its environment.The student will explore these processes in two contexts: visually-driven behaviour in zebrafish and feeding behaviour in worms. In zebrafish, as in all vertebrates, excitatory synapses convey the visual signal through the retina to downstream circuits controlling behaviours such as prey-capture and the optomotor response. These behaviours are plastic and we will investigate how neuromodulators such as dopamine and serotonin act on synapses to adjust them. In the context of the worm C. elegans the project and its collaboration will investigate a second-to-second interaction between two distinct glutamate-dependent circuits that pivot feeding behaviour. The student will image the dynamics of this circuit as it controls feeding behaviour.

第一次旋转-突触传递的光学成像：一种探索行为的功能决定因素的变革性技术。3D实时成像是理解神经系统行为控制等生物过程的关键，但要快速实现这一目标具有挑战性。一种方法是选择性平面照明显微镜（SPIM），它使用在高速相机上成像的薄片光：光片和成像物镜的快速z轴运动允许在3D中高速观察。我们将开发一种具有改进分辨率的SPIM，使用双光子贝塞尔光束激发通过小型活体动物（如幼体斑马鱼或秀丽隐杆线虫）的大脑成像。该仪器是英国第一个，将允许学生开发一个基于可视化的项目，该项目使用荧光谷氨酸报告基因iGlUSnFR在神经元之间的突触连接中传输信号的动态。这种变革性的技术将揭示信息是如何在动物与环境相互作用时流经大脑的。学生将在两种情况下探索这些过程：斑马鱼的视觉驱动行为和蠕虫的摄食行为。和所有脊椎动物一样，斑马鱼的兴奋性突触通过视网膜将视觉信号传递到下游回路，控制诸如捕获猎物和视运动反应等行为。这些行为是可塑的，我们将研究多巴胺和血清素等神经调节剂如何作用于突触来调节它们。在秀丽隐杆线虫的背景下，该项目及其合作将研究两个不同的谷氨酸依赖回路之间的秒对秒相互作用，这些回路决定了摄食行为。学生将对这个电路的动态成像，因为它控制进食行为。