Determination of RNA editing sites in black pine chloroplast mRNA

黑松叶绿体 mRNA 中 RNA 编辑位点的测定

• 批准号: 07640873
• 负责人: NAKASHIMA Keiko
• 金额: $ 1.47万
• 依托单位: Sugiyama Jogakuen University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07640873/
• 关键词: black pine; chloroplast; RNA editing; mRNA; NRA editing; RNAエディティング(RNA editing); クロロプラスト(chloroplast); マツ(black pine); 読み枠(reading frame); コドン(codon); トランジション(transition)

We have estimated nearly three hundreds of RNA editing sites in chloroplast genome [119,707bp] from black pine [Pinus thunbergii] by aligning proteinous gene areas [62 protien gene and conserved ORFs] with those of other chloroplast genomes. Finally, we have identified twenty six C-to-U RNA editing sites and those include following cases of importance.1. New ORF formation by ACG to AUG editing ; This clearly shows the existence of ORF that can't be estimated from DNA sequence itself.2. CAA to UAA editing introducing terminal code [two cases] ; This also contributes modifying reading frame of proteinous gene of concern in maturing process of mRNA.3. New editing types of GCA to GUA,CCC to UCC,ACU to AUU and CUG to UUG ; These findings come from entire search of estimated editing sites on the genome.These results indicate the importance of RNA editing in post-transcriptional event in higher-plant chloroplast gene expression much more than expected.We find no concensus sequence in surounding area of editing sites while we have performed the multiple alignment for all the found editing sites and for grouped sites of the sasme editing pattern. Thus the mechanism for identifying the RNA editing site [s] is remained unknown.

通过蛋白质基因区[62个蛋白质基因和保守ORF]与其它叶绿体基因组的比对，我们估计了黑松叶绿体基因组[119，707 bp]中近300个RNA编辑位点。最后，我们确定了26个C-to-U RNA编辑位点，包括以下重要的情况.通过ACG到AUG编辑形成新的ORF;这清楚地表明了不能从DNA序列本身估计的ORF的存在。CAA到UAA编辑引入末端密码[两例] ;这也有助于在mRNA成熟过程中修饰相关蛋白质基因的阅读框架. GCA到GUA、CCC到UCC、ACU到AUU和CUG到UUG的新编辑类型;这些发现来自于对基因组上估计的编辑位点的整个搜索。这些结果表明RNA编辑在转录后事件中的重要性，植物叶绿体基因的表达比预期的要高得多，我们对所有发现的编辑位点进行了多重比对，但在编辑位点周围没有发现一致的序列sasme编辑模式的站点和分组站点。因此，识别RNA编辑位点的机制仍然未知。

项目成果

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