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Mechanism of epileptogenesis of the Mongolian gerbil, an animal model of epilepsy.

癫痫动物模型蒙古沙鼠的癫痫发生机制。

基本信息

批准号：
07640917
负责人：
OHSHIMA Akiko
金额：
$ 1.54万
依托单位：
Institute for Developmental Research, Aichi Human Service Center
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07640917/
关键词：
Gerbil Epileptogenesis スナネズミ発作形成

项目摘要

The Mongolian gerbil (Meriones unguiculatus) is a genetical animal model of epilepsy. Using a seizure-sensitive strain we had established previously, we found that the rhythmical backward movement of the pinnae occurring after posture change, a strong inducer of the seizure, was the early phase of the epiletogenesis and that the vestibular cortex which brings the backward movement of the pinnae by electrical stimulation. A telemeter machine showed that the repetitive injection of pentylentetrazole and other events which induce the seizure-related behaviors induced a sharp wave at this area accompanied with similar ear movement, which may suggest that this area participates in the early phase of epileptogenesis. How the motor cortex works is now under investigation. The animals of a seizure-resistant strain established by Dr. Murashima (Tokyo Institute of Psychiatry) did not show the ear movement after posture change but their facial nerve was found to enter into the ear muscles similarly to the sensitive strain, which suggests other component(s) in the pathway between the vestibular end-origan and the ear muscle is responsible for the difference observed. A protein like P70, a 70kDa protein considered to be involved in the epileptogenesis of cobalt-treated rat, was analyzed and found that it changed its location to the plasma membrane of the neurons where it often colocalized with the intrinsic fluorescent materials in the cerebral cortex during the term of the epileptogenesis. This protein seems to resemble albumin of which amino acid sequence was determined from cDNA in this study. Albumin mRNA was found in the brain of the 3-month-old gerbils. Thus, modified albumin may participate to change the properties of ion channels at the cell periphery like P70.

长爪沙鼠（Meriones unguiculatus）是一种遗传性癫痫动物模型。利用我们以前建立的前庭敏感品系，我们发现，姿势改变后发生的有节奏的向后运动的小鳍，一个强大的诱导剂的癫痫发作，是epiletogenesis的早期阶段，前庭皮质，使向后运动的小鳍由电刺激。遥测仪显示，重复注射戊四氮和其他诱发癫痫相关行为的事件在该区域引起尖波，并伴有类似的耳运动，这可能表明该区域参与了癫痫发生的早期阶段。运动皮层是如何工作的，目前正在研究中。Murashima博士（东京精神病学研究所）建立的前庭抗性品系的动物在姿势改变后没有显示出耳朵运动，但发现它们的面神经与敏感品系类似地进入耳肌，这表明前庭起源终末和耳肌之间的通路中的其他成分是造成所观察到的差异的原因。对一种被认为与钴处理大鼠癫痫发生有关的70kDa蛋白质P70进行了分析，发现它在癫痫发生期间改变了其定位于神经元质膜的位置，在那里它经常与大脑皮层中的内在荧光物质共定位。该蛋白似乎类似于白蛋白，在本研究中从cDNA确定了其氨基酸序列。3月龄沙鼠脑内有白蛋白mRNA表达。因此，修饰的白蛋白可能参与改变细胞周边离子通道的性质，如P70。