Is uric acid just a waste product? -Its role in antioxidation system in insects-

1.建立了一种高灵敏度的昆虫脂质过氧化物检测系统。该系统采用高效液相色谱和化学发光检测器（CL-HPLC）。甘油三酯过氧化物的检测限为1 pmol。用Sep-pak NH_2预处理可有效地去除昆虫样品中的化学发光抑制剂。2.用含光毒性物质赤藓红B的饲料喂养洋葱蝇迪利亚幼虫，用荧光灯照射，使其产生氧化应激，与对照昆虫（不含赤藓红B）相比，幼虫含有更大量的脂质过氧化物。3.使用家蚕（Bombyx mori）检测尿酸对抗氧化应激的作用。当黄嘌呤脱氢酶抑制剂别嘌呤醇加入家蚕人工饲料中时，以这种饲料生长的B体内尿酸的量降低到检测不到的水平。在紫外线照射下，尿酸耗竭的蚕的寿命比正常昆虫明显缩短。缺乏尿酸的蚕也表现出对光毒性物质虎红产生的氧化应激的抵抗力下降。

1.A highly-sensitive detection system for peroxides in a lipid fraction from an insect was developed. The system employs a high performance liquid chromatography and a chemiluminescence detector (CL-HPLC). The detection limit for triglyceride peroxide was 1 pmol. Samples from an insect body were found to contain unknown inhibitor(s) for chemiluminescence, but it was efficiently removed by a pretreatment with Sep-pak NH_2.2.The larvae of the onion fly, Delia antiqua was fed with a diet containing a phototoxicant, Erythrosine B.When the larvae was given an oxidative stress by illuminating them with a fluorescent lamp, the larvae contained a larger amount of lipid peroxide compared with the control insects (without Erythrosine B).3.The role of uric acid against the oxidative stress was examined using the silkworm, Bombyx mori. When an inhibitor of xanthine dehydrogenase, allopurinol, was added to the artificial diet of B.mori, the amount of uric acid in the body of B.mori grown on this diet was decreased to a nondetectable level. Under illumination with ultraviolet light, the silkworms depleted with uric acid showed a significantly shorter life span than the normal insects. The silkworms depleted with uric acid also showed decreased resistance against oxidative stress produced by a phototoxicant, rose bengal.