MATURATION AND SPAWNING OF WILD TIGER PUFFER TAKIFUGU RUBRIPES AND MECHANISM OF MATURATION ELUCIDATED BY CULTURED ONE

1995年：为了给养殖业提供受精卵，本研究采用激素诱导红鳍东方鲀（Takifugu rubripes）卵巢成熟和排卵的方法。用LHRH-a胆固醇颗粒（LHRH-a 400 mg/kg）对卵巢有卵黄堆积的3龄养殖河豚鱼进行胚胎植入，成功地诱导了卵巢成熟。然后用HCG（500 IU/kg）和鲑鱼垂体匀浆（7 mg/kg）联合单次注射，以同步排卵。在实验I（卵母细胞直径为900 mm的鱼）中，从所用的7条鱼中的5条获得受精卵。在实验II（鱼与卵母细胞之间的直径800和900毫米），受精卵获得8的10条鱼tereated。相反，在作为常规方法的实验III中（在没有LHRH-a处理的情况下以5天间隔重复注射HCG+SP），从5条鱼中的2条获得受精卵。平均生育力 关于我们 三个实验的转化率分别为55.1%、53.6%和11.0%。在Exp. I、II期排卵主要发生在HCG+SP注射后96 ~ 120 h。1996年：本研究旨在为红鳍东方鲀（Takifugu ruberipes）成熟诱导激素（MIH）的真实性提供直接证据。为此，我们在卵母细胞发育的四个不同阶段使用无卵黄卵泡细胞。将无卵黄卵泡细胞与17 <14>C-标记的17 α-羟孕酮孵育2小时，然后色谱分离放射性类固醇。对产生的放射性代谢产物进行了鉴定，并检测了其体外诱导卵母细胞生殖囊泡破裂（GVBD）的能力。此外，通过监测最终卵母细胞成熟过程中的血清类固醇水平，研究了MIH候选物的体内产生。本研究结果为20 b-S是河豚鱼体内天然存在的MIH提供了证据。少

1995 : We developed the effective method of induction of ovarian maturation and ovulation of the cultured tiger puffer Takifugu rubripes by hormonal treatments in order to supply the fertilized eggs for aquaculture. The LHRH-a cholesterol pellet (LHRH-a 400 mg/kg) implantation successully induced overian maturation for three-year-old cultured tiger puffer which had yolk accumulated ovaries. Thereafter single injection of HCG (500IU/kg) combined with chum salmon pituitary (SP) homogenate (7 mg/kg) was applied to the fish to sinchronize ovulation among fish. In Experiment I (fish with oocytes *900 mm in diameter), fertilized eggs were obtained from 5 of 7 fish used. In Experiment II (fish with oocytes between 800 and 900 mm in diameter), fertilized eggs were obtained from 8 of 10 fish tereated. On the contrary, in Experiment III as a conventional method (repeated injection of HCG+SP at 5 days interval without LHRH-a treatment), fertilized eggs were obtained from two of 5 fish. Mean ferti … More lization rates in the three experiments were 55.1,53.6, and 11.0%, respectively. In Exp. I and II,ovulation was mainly occurred between 96 and 120 hours after HCG+SP injection. Thus, a thchnique for obtaining eggs of high quality from the cultured tiger puffer was developed.1996 : The study was designed to obtain derect evidence for the authentic maturation-inducing hormone (MIH) of tiger puffer, Takifugu ruberipes. To this end, we used yolk free follicle cells at four different stages of oocyte development. The yolk free follicle cells were incubated with the ^<14>C-labeled 17a-hydroxyprogesterone for 2hr, followed by chromatographic separation of the radioactive steroids. The radioactive metabolites produced were identified and tested for their ability to induce germinal vesicle breakdown (GVBD) in oocytes in vitro. Furthermore, in vivo production of candidates for MIH was investigated by monitoring the serum steroid levels during final oocyte maturation. The results obtained in this study provide evidence that 20b-S is the naturally occurring MIH in tiger puffer. Less