Distribution and function of tetrodotoxin-binding proteins in the pufferfish Takifugu rubripes -elucidation from a viewpoint of sexual maturation

河豚毒素结合蛋白在红鳍东方鲀中的分布和功能——从性成熟的角度阐明

• 批准号: 23580282
• 负责人: ARAKAWA Osamu
• 金额: $ 3.41万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580282/
• 关键词: テトロドトキシン; フグ; ツムギハゼ; テトロドトキシン結合性タンパク質（PSTBP）; 成熟; トラフグ; 交雑フグ; 肝量指数（HSI）; 生殖腺体指数（GSI）; クサフグ; マフグ; PSTBP

Various tetrodotoxin (TTX) administration experiments using non-toxic cultured pufferfish, and investigations on internal TTX distribution of several wild pufferfish species and the goby Yongeichthys criniger demonstrated that TTX kinetics inside the body changes depending on the skin/liver development due to growth, and ovary development/maturation due to sexual maturation in these TTX-bearing fish. Moreover, searches for homologous genes of the TTX-binding protein (PSTBP) on several pufferfish species indicated that Takifugu rubripes possesses 3 candidate genes, 2 of which actually exist as proteins in the blood plasma, and that the other 3 toxic species also have 2-domain type candidate genes, and the difference in their sugar chain binding sites may be related to the toxin accumulation ability.

使用无毒养殖河豚进行的各种河豚毒素 (TTX) 施用实验，以及对几种野生河豚和虾虎鱼体内 TTX 分布的研究表明，体内 TTX 动力学的变化取决于生长引起的皮肤/肝脏发育，以及这些携带 TTX 的鱼性成熟引起的卵巢发育/成熟。此外，在几种河豚身上寻找TTX结合蛋白（PSTBP）同源基因表明，红鳍东方鲀拥有3个候选基因，其中2个实际上以蛋白质形式存在于血浆中，而其他3个有毒物种也有2个结构域型候选基因，其糖链结合位点的差异可能与毒素积累能力有关。

项目成果

Growthassociated changes in internal tetrodotoxin distribution and skin structure in three species of pufferish

三种河豚体内河豚毒素分布和皮肤结构与生长相关的变化

• 发表时间: 2013
• 作者: R. Tatsuno;T. Mine;Y. Yamanaka;K. Ikeda;T. Takatani;O. Arakawa
• 通讯作者: O. Arakawa

最近日本で問題になっている魚介毒

最近在日本成为一个问题的海鲜毒物

• 发表时间: 2011
• 作者: R. Tatsuno;M. Shikina;Y. Shirai;T. Takatani;O. Arakawa;朴紅・坂下明彦;小瀧裕一・木田龍介・影山一成・原口浩一・Lundholm Nina;荒川 修
• 通讯作者: 荒川 修

Transfer profile of intramuscularly administered tetrodotoxin to different ages of artificial hybrid pufferfish

不同龄期人工杂交河豚肌肉注射河豚毒素的转移谱

• 作者: S. Niina;T. Araki;Y. Yamanaka;J. Wang;R. Tatsuno;O. Arakawa and T. Takatani
• 通讯作者: O. Arakawa and T. Takatani

Comparison of the localization of tetrodotoxin between wild pufferfish Takifugu rubripes juveniles and hatchery-reared juveniles with tetrodotoxin administration

• DOI: 10.1016/j.toxicon.2013.05.018
• 发表时间: 2013-09-01
• 期刊: TOXICON
• 影响因子: 2.8
• 作者: Okita, Kogen;Takatani, Tomohiro;Sakakura, Yoshitaka
• 通讯作者: Sakakura, Yoshitaka

Change in the transfer profile of orally administered tetrodotoxin to non-toxic cultured pufferfish Takifugu rubripes depending of its development stage

• DOI: 10.1016/j.toxicon.2013.01.011
• 发表时间: 2013-04-01
• 期刊: TOXICON
• 影响因子: 2.8
• 作者: Tatsuno, Ryohei;Shikina, Miwako;Arakawa, Osamu
• 通讯作者: Arakawa, Osamu