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Post-sequencing functional studies on the pufferfish (Takifugu rubripes) genome

河豚（Takifugu rubripes）基因组的后测序功能研究

基本信息

批准号：
14104008
负责人：
WATABE Shugo
金额：
$ 72.38万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (S)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2006
项目状态：
已结题

项目摘要

1.Sequencing of 24,398 ESTs generated from 15 different adult and juvenile torafugu tissues resulted in 10,116 gene tags when compared with the genome data. The EST assemblies showed strong conservation with those of zebrafish, although significant differences remained between the two species.2.Muscle specific genes, myosin heavy chain (MYH) and tropomyosin (TPM), and genes encoding myogenic regulatory factors (MyoD family) such as MyoD and myogenin were analyzed in silico and in vivo. Comparison with mammalian counterparts revealed that although the number of the MyoD family genes was almost the same, torafugu had MYHs and TPMs about twice as many as mammalian counterparts, which suggests genome duplication in teleost.3.A total of 28 MYHs comprising 13 fast skeletal, 5 cardiac, 2 slow skeletal, 1 superfast, 2 smooth and 5 nonmuscle, were found in torafugu.4.Torafugu fast skeletal MYHs were distributed across five genomic regions, three of which contained MYH clusters. While human 6 fast skeletal MYHs formed one cluster, its syntenic region in torafugu was duplicated, although each locus contained a single MYH. These results suggest that fast skeletal MYHs evolved independently in teleosts and tetrapods after they had diverged from a common ancestor.5.Bacterial isolates from the torafugu skin were agglutinated by pufflectin, but could not adhere to the torafugu skin.6.pIgR was isolated as an transporter of the skin mucus IgM and, unlike mammals, torafugu pIgR was expressed not only in the intestine but also in the skin. A fragment of pIgR could bind to tetrameric IgM in the skin mucus.7.Marker genes of antigen presenting cells, CD11c, CD40 and MHC class IIα, and genes encoding costimulatory B7 molecules, B7-H1, B7-H3 and B7-H4, isolated from torafugu represent the first of these kinds from teleosts.

1.从15种不同的成年和幼年托拉富古鱼组织中产生的24,398个ESTs测序结果显示，与基因组数据相比，有10,116个基因标签。EST序列与斑马鱼具有较强的保守性，但仍存在显著差异。对肌肉特异性基因myosin heavy chain （MYH）和tropomyosin （TPM）以及MyoD和myogenin等肌生成调节因子（MyoD家族）的编码基因进行了计算机和体内分析。与哺乳动物相比较发现，尽管MyoD家族基因的数量几乎相同，但托拉富古鱼myh和TPMs的数量是哺乳动物的两倍，这表明硬骨鱼存在基因组复制。在torafugu中共发现28个myh，其中快速骨骼13个，心脏5个，慢骨骼2个，超高速1个，平滑肌2个，非肌肉5个。Torafugu快速骨骼MYH分布在5个基因组区域，其中3个包含MYH集群。虽然人类6个快速骨骼MYH形成了一个簇，但其在torafugu的同区是重复的，尽管每个位点包含一个MYH。这些结果表明，在硬骨鱼和四足动物从共同的祖先分化出来之后，它们的快速骨骼myh是独立进化的。pufflectin的用法和样例：pufflectin的用法和样例：pIgR作为皮肤黏液IgM的转运体被分离出来，与哺乳动物不同的是，torafugu pIgR不仅在肠道中表达，而且在皮肤中表达。pIgR片段可与皮肤黏液中的四聚体IgM结合。抗原呈递细胞CD11c、CD40和MHCⅱ类α的标记基因，以及编码共刺激B7分子B7- h1、B7- h3和B7- h4的基因，在硬骨鱼中尚属首次。

项目成果

期刊论文数量（76）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Characterization and expression analysis of the CD3ε and CD3_Y/δ in fugu, Takifugu rubripes.

红鳍东方鲀 CD3ε 和 CD3_Y/δ 的表征和表达分析。

DOI：
发表时间：
2005
期刊：
Immunogenetics 57
影响因子：
0
作者：
Araki;K.;Suetake;H.;Kikuchi;K.;Suzuki;Y.
通讯作者：
Y.

Identification and characterization of Fugu orthologues of mammalian interleukin-12 subunits

DOI：
10.1007/s00251-003-0582-9
发表时间：
2003-07
期刊：
Immunogenetics
影响因子：
3.2
作者：
Y. Yoshiura;I. Kiryu;A. Fujiwara;H. Suetake;Yuzuru Suzuki;T. Nakanishi;M. Ototake
通讯作者：
Y. Yoshiura;I. Kiryu;A. Fujiwara;H. Suetake;Yuzuru Suzuki;T. Nakanishi;M. Ototake

"トラフグを中心に魚類体表の生体防御機構をさぐる"海洋生命系のダイナミクス・シリーズ第2巻海洋生物の機能生命は海にどう適応しているか(竹井祥郎編)

《以虎河豚为中心研究鱼类体表的生物防御机制》海洋生物系统动力学系列第2卷海洋生物的功能生命如何适应海洋（武井义郎主编）

DOI：
发表时间：
2005
期刊：
影响因子：
0
作者：
H.Oguri;et al.;池田大介ら;鈴木譲ら
通讯作者：
鈴木譲ら

Isolation of microsatellite markers by in silico screening implicated for genetic linkage mapping in Japanese pufferfish Takifugu rubripes

通过计算机筛选分离微卫星标记，用于日本河豚红鳍东方鲀的遗传连锁图谱

DOI：
发表时间：
2004
期刊：
Fish.Sci. 70
影响因子：
0
作者：
Y.Nakamura;M.Ando;M.Seoka;K.Kawasaki;Y.Sawada;S.Miyashita;T.Okada;H.Kumai;Y.Tsukamasa.;Megumi Hatori et al.;Furukawa S et al.
通讯作者：
Furukawa S et al.

Identification of three isoforms for mitochondrial adenine nucleotide translocator in the pufferfish Takifugu rubripes