Ultrastructural and ultracytochemical observations on differentiation and fate of liver macrophages of the mouse embryo

小鼠胚胎肝巨噬细胞分化和命运的超微结构和超细胞化学观察

• 批准号: 07670043
• 负责人: SASAKI Kazunobu
• 金额: $ 1.47万
• 依托单位: KAWASAKI MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670043/
• 关键词: Macrophages; Liver Hemopoiesis; mouse embryos; Primitive erythrocytes; Erythroblastic islands; Enucleation; Ultracytochemistry; 超微組織化学; 類洞マクロファージ; 赤芽球島中心マクロファージ; 卵黄嚢造血

Embryonic liver macrophages are derived from intravascular macrophages. Before the initiation of liver hemopoiesis, embryonic peripheral blood contained a few free phagocytes as early as 10 days of gestation. Based on fine structural criteria, lntravascular phagocytes were considered to be specialized scavenger macrophages with the function of clearing the blood, and they are the precursor cells of sinusoidal macrophages at the onset of liver hemopoiesis.Sinusoidal macrophages move and differentiate into central macrophages of erythroblastic islands. Our ultrahistochemical and ultrastructural studies showed that the central cells in liver erythroblastic islands do not originate from hepatocytes but from sinusoid macrophages and that SBA binding ability is closely concerned with liver macrophage maturation.Changes of phagocytotic targets of liver macrophage take place in developmental saquence of liver hemopoiesis. Aged primitive erythrocytes, cell fragments of primitive erythrocytes, nuclei expelled from definitive erythroblasts and dying polymorphonuclear leukocytes were removed from fetal liver by macrophages at the various stages of liver hemopoiesis.Erythroblastic islands disappear in early neonatal liver and sinusoidal macrophages remain in adult livers. Neonatal liver contained two kinds of macrophages ; sinusoidal scavenger macrophages and central macrophages of erythroblastic islands. After 5 days of age, erythroblasts gradually left the islands, and the macrophages which lost surrounding erythroblasts became starfish-shaped due to many long twisted cytoplasmic projections. Their nuclei became shrinked and condensed, and the cytoplasm was fragmental and removed.

胚胎肝巨噬细胞来源于血管内巨噬细胞。在肝脏造血开始之前，早在妊娠10天胚胎外周血就含有少量游离的吞噬细胞。根据精细结构标准，血管内吞噬细胞被认为是具有清除血液功能的特化清道夫巨噬细胞，是肝脏造血开始时窦状巨噬细胞的前体细胞。窦状巨噬细胞移动并分化为红细胞岛中央巨噬细胞。我们的超组织化学和超微结构研究表明，肝红细胞岛的中央细胞并非来源于肝细胞，而是来源于肝窦巨噬细胞，并且SBA结合能力与肝巨噬细胞的成熟密切相关。肝巨噬细胞吞噬靶点的变化发生在肝造血的发育过程中。衰老的原始红细胞、原始红细胞的细胞碎片、定形红细胞排出的细胞核和垂死的多形核白细胞在肝脏造血的各个阶段被巨噬细胞从胎儿肝脏中清除。早期新生儿肝脏中的红细胞岛消失，成人肝脏中仍保留窦状巨噬细胞。新生儿肝脏含有两种巨噬细胞；正弦清道夫巨噬细胞和红细胞岛中央巨噬细胞。 5日龄后，有红细胞逐渐离开岛屿，失去周围有红细胞的巨噬细胞由于许多长而扭曲的细胞质突起而变成海星状。它们的细胞核收缩并浓缩，细胞质破碎并被去除。

项目成果

Iwatsuki, H.: "Membrane-bound glycoconjugates of tetal mouse erythropoietic cells with special reference to phagocytosis by hepatic macrophages." Histochem Cell Biol. 104. 227-286 (1995)

Iwatsuki, H.：“小鼠红细胞生成细胞的膜结合糖复合物，特别是肝巨噬细胞的吞噬作用。”

Sasaki, K: "Ultrastructure of Intravascular And Coelomic Scavenger Macrophages Of THe Mouse Embryo" Arch. Histol. Cytol.58. 549-556 (1995)

Sasaki，K：“小鼠胚胎血管内和体腔清道夫巨噬细胞的超微结构”Arch。

Iwatsuki, H: "Membrane-bound glycoconjugates of fetal mouse erythropoietic cells with special reference to phagocytosis by hepatic macrophages." Histochem. Cell Biol.104. 227-286 (1995)

Iwatsuki，H：“胎儿小鼠红细胞生成细胞的膜结合糖复合物，特别是肝巨噬细胞的吞噬作用。”

須田満寿美: "生後早期のマウス肝臓における中性好性顆粒白血球の細胞死:組織学的観察." 川崎医学会雑誌. 21. 71-80 (1995)

Masumi Suda：“出生后早期小鼠肝脏中的中性粒细胞死亡：组织学观察”，川崎医学会杂志 21. 71-80 (1995)。

Iwatsuki, H: "Origin of central cells of erythroblastic islands in fetal mouse liver : Ultrahistochemical studies of membrane‐bound glycoconjugates." Histochem Cell Biol. (in press). (1997)

Iwatsuki，H：“胎鼠肝脏中成红细胞岛的起源：膜结合糖复合物的超组织化学研究”（出版中）。