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Liver hematopoiesis and hematopoietic microenvironment - three dimensional reconstruction and immunohistochemical observation of CD markers

肝脏造血及造血微环境——CD标记物的三维重建及免疫组化观察

基本信息

批准号：
12670033
负责人：
SASAKI Kazunobu
金额：
$ 2.11万
依托单位：
KAWASAKI MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

In fetal liver hematopoiesis, four stages were identified : Stage I, onset of hematopoiesis at 10 days, Stage II, expansion of the volume of the hematopoietic compartment at ll and 12 days, Stage III, peak in the volume of the hematopoietic compartment at 13 and 14 days, and Stage IV, involution of hematopoiesis after 15 days. During Stages I-II, hematopoietic stem cells appeared to move from the sinusoidal lumina into primitive hepatic cell cords to give rise to colonies among hepatoblasts. At Stage III, the hematopoietic colonies formed ellipsoidal foci as a structural unit of hematopoiesis, and then the erythropoietic foci appeared as cords. At Stage IV, these cord-shaped foci became disrupted, and round solitary foci appeared within the hepatic cell cords on meandering sinusoids. Hematopoietic foci in Stage IV contains numerous granulocytes and lymphocytes as well as erythroblasts.The hepatoblast volumes appeared constant until 13 days of gestation, and then showed a gradual increa … More se. Hepatoblasts at stage HI made contact with one another by short cytoplasmic projections. ＆cadherin mediated adherens junctions were found. At stage H-III, in addition to the adherens junctions, small desmosomes appeared to bind hepatoblasts together, and section profiles of biliary canaliculi could be recognized between hepatoblasts. At the IE stage, both tight junctions and gap junctions appeared around the biliary canaliculi, and four types of specialized junctions, i. e., adherens junctions, desmosomes, tight junctions and gap junctions, appeared fully organized.In order to differentiate lymphocytes from myeloid hematopoietic cells and to determine lymphocytesubclasses, anti-mouse anti-CD monoclonal antibodies were used. In control staining, the antibodies were ableto stain T or B lymphocytes specifically in adult spleens, but, in fetal and neonatal livers, specific results could no be obtained. Immunohistochemical stainings with the anti-mouse monoclonal antibodies were considered to be future tasks in mouse fetal organs. Less

在胎肝造血中，确定了四个阶段：阶段I，在10天时开始造血，阶段II，在11天和12天时造血区室的体积扩大，阶段III，在13天和14天时造血区室的体积达到峰值，以及阶段IV，在15天后造血退化。在阶段I-II，造血干细胞似乎从窦腔移动到原始肝细胞索中，在肝母细胞中产生集落。III期造血细胞集落形成椭球形集落，成为造血的结构单位，随后红细胞集落呈条索状。在第四阶段，这些索状病灶被破坏，圆形孤立病灶出现在蜿蜒的肝窦上的肝细胞索内。IV期造血灶内有大量的粒细胞、淋巴细胞和成红细胞，肝细胞体积在妊娠13天前基本不变，13天后逐渐增大，13天后逐渐缩小，13天后逐渐缩小。 ...更多信息 se. HI阶段的肝母细胞通过短的细胞质突起相互接触。在H-III期，除粘附连接外，小桥粒也与肝母细胞结合在一起，肝母细胞间可见胆管的断面轮廓。在IE期，胆管周围既有紧密连接，也有缝隙连接，并出现四种特化连接，即：例如，粘附连接、桥粒、紧密连接和间隙连接组织完整。为了区分淋巴细胞和骨髓造血细胞并确定淋巴细胞亚类，使用了抗小鼠抗CD单克隆抗体。在对照染色中，抗体能够特异性地染色成人脾脏中的T或B淋巴细胞，但在胎儿和新生儿肝脏中，不能获得特异性结果。用抗小鼠单克隆抗体进行免疫组织化学染色被认为是小鼠胎儿器官的未来任务。少