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Cytometrical analysis of blood monocytes : comparative studies of adult peripheral blood and embryonic hemopoietic organs.

血液单核细胞的细胞计数分析：成人外周血和胚胎造血器官的比较研究。

基本信息

批准号：
02670001
负责人：
SASAKI Kazunobu
金额：
$ 1.34万
依托单位：
Kawasaki Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670001/
关键词：
Monocytes Macrophages Lymphocytes Blood Thymus Liver Cytometry Ultrastructure マウス

项目摘要

The purpose of this study was first to examine cytometrically monocutes in the buffy coat of peripheral blood under light and electron microscopy and second to compare monocutes with macrophage precursors during an early embryonic life.1. Monocutes in peripheral bloodSemithin light and electron microscopic observations revealed that the cell size and H-C ratio were not the first criterion for identification of monacytes in peripheral blood in the mouse. Small monocutes and large granular lymphocytes in the buffy coat of peripheral blood are largely overlapped in cell size distribution. Ultrastructurally, however, the occurrence of subsurface vacuoles, long cytoplasmic projections and the abundance of cytoplasmic granules in monocutes are useful features for the distinction between iiononuclear cells in the peripheral blood.2. Fetal liverAt 11-12 days gestation, liver sinusoids contained many scavenger macrophages showing active phagocytosis to primitiveerythroblasts from yolk sac. Mononuclear cells, morphologically identified as monocutes, first appeared in embryonic liver at 13 days gestation. The time of appearance of monocutes must be taken into account in pattern of macrophage differentiation in early embryos.3. Fetal ThymusIn the embryonic thymus, macrophages and monocutes were present as early as 15 days gestation. They first appeared in the perivascular space which was continuous with the mesenchyme surrounding the thymus. The precursors of thymic macrophages are not from the yolk sac but monocutes from the fetal liver.

本研究的目的是首先在光镜和电镜下检查外周血血沉棕黄层中的单细胞计数，其次比较早期胚胎生命中的单细胞计数与巨噬细胞前体。外周血单核细胞半薄光镜和电镜观察表明，细胞大小和H-C比值不是鉴定小鼠外周血单核细胞的首要标准。外周血血沉棕黄层中的小单核细胞和大颗粒淋巴细胞在细胞大小分布上大部分重叠。超微结构上，单核细胞亚表面空泡的出现、胞质长突起和胞质颗粒的丰富程度是区分外周血单核细胞的有用特征.胎肝孕11-12天，肝窦内含有大量的清道夫巨噬细胞，对卵黄囊内的成红细胞有吞噬作用。单核细胞，形态学鉴定为单核细胞，第一次出现在胚胎肝脏在妊娠13天。在研究早期胚胎巨噬细胞分化模式时，应考虑单核细胞出现的时间.胎儿胸腺在胚胎胸腺中，巨噬细胞和单核细胞早在妊娠15天就已存在。它们首先出现在血管周围空间，该空间与胸腺周围的间充质连续。胸腺巨噬细胞的前体不是来自卵黄囊，而是来自胎肝的单核细胞。