Cytometrical analysis of blood monocytes : comparative studies of adult peripheral blood and embryonic hemopoietic organs.

这项研究的目的是首先在光镜和电子显微镜下观察外周血棕黄色涂层中的单核细胞，然后比较单核细胞和早期胚胎生命中的巨噬细胞前体1。外周血中单核细胞的半薄光和电子显微镜观察表明，细胞大小和H/C比不是鉴定小鼠外周血中单核细胞的第一标准。外周血棕黄色涂层中的小单体淋巴细胞和大颗粒淋巴细胞在细胞大小分布上有很大的重叠。然而，在超微结构上，单核细胞中出现亚表面空泡、长的细胞质突起和丰富的细胞质颗粒是区分外周血中离子核细胞的有用特征。胎肝在妊娠11~12天，肝窦内含有大量清道夫巨噬细胞，对卵黄囊原始红细胞具有主动吞噬作用。单个核细胞，形态上被鉴定为单核细胞，在妊娠13天时首次出现在胚胎肝脏中。在早期胚胎的巨噬细胞分化模式中，必须考虑单倍体出现的时间。胎儿胸腺在胚胎胸腺中，巨噬细胞和单核细胞早在妊娠15天就已存在。它们最早出现在血管周围，与胸腺周围的间质相连。胸腺巨噬细胞的前体不是来自卵黄囊，而是来自胎肝。

The purpose of this study was first to examine cytometrically monocutes in the buffy coat of peripheral blood under light and electron microscopy and second to compare monocutes with macrophage precursors during an early embryonic life.1. Monocutes in peripheral bloodSemithin light and electron microscopic observations revealed that the cell size and H-C ratio were not the first criterion for identification of monacytes in peripheral blood in the mouse. Small monocutes and large granular lymphocytes in the buffy coat of peripheral blood are largely overlapped in cell size distribution. Ultrastructurally, however, the occurrence of subsurface vacuoles, long cytoplasmic projections and the abundance of cytoplasmic granules in monocutes are useful features for the distinction between iiononuclear cells in the peripheral blood.2. Fetal liverAt 11-12 days gestation, liver sinusoids contained many scavenger macrophages showing active phagocytosis to primitiveerythroblasts from yolk sac. Mononuclear cells, morphologically identified as monocutes, first appeared in embryonic liver at 13 days gestation. The time of appearance of monocutes must be taken into account in pattern of macrophage differentiation in early embryos.3. Fetal ThymusIn the embryonic thymus, macrophages and monocutes were present as early as 15 days gestation. They first appeared in the perivascular space which was continuous with the mesenchyme surrounding the thymus. The precursors of thymic macrophages are not from the yolk sac but monocutes from the fetal liver.