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Studies on intraepithelial lymphocytes and cytokine production in the protection against nematode infection

上皮内淋巴细胞和细胞因子产生预防线虫感染的研究

基本信息

批准号：
07670270
负责人：
ABE Tatsuya
金额：
$ 1.47万
依托单位：
Akita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670270/
关键词：
parasite protection intraepitheliallymphocytes cytokine mouse flow cytometry S.ratti N.brasiliensis

项目摘要

The mechanism of worm expulsion, spontaneous cure, of the intestinalnematodes is recently understood that the direct effector cells against parasites are selective among various intestinal nematode species. To generalize the mechanism of worm expulsion, we need to understand the mechanism for regulating direct effector cells. We focused on intestinalintraepithelial cells (IEL), and tried to study on T cell subsets and cytokine production in S.ratti (Sr) and N.brasiliensis (Nb) -infection by flow cytometry and RT-PCR.We examined also on mesenteric lymph node cells by those methods. Mean recovery of IEL from Sr-infected and Nb-infected mice were less than 40% and 6% of IEL from normal mice, respectively. Percentage of CD3^+, CD4^+, CD8^+ and gammadeltaTCR^+ cells was not different basically between IEL from Sr-infected and that from Nb-infected mice. Th2 type cytokines, IL-4 and IL-3 mRNA were detected clearly in IEL from Sr-infected mice on day 7 but not in normal IEL.Th1 type cytokine, … More IFN-gamma mRNA was detected in normal IEL and hardly changed in the infection. IEL probably play a role on the expulsion of Sr, because IL-3 mRNA,a mast cell growth factor messenger, was increased in IEL in the infection. We could not examine the expression of mRNA in IEL from Nb-infected mice.Intracellularcytokines in mesenteric lymph node cells were detected by flow cytometry. IL-3 containing cells increased in the mesenteric lymph node both in Sr-and Nb-infection. IL-4 or IFN-gamma containing cells were not different basicallyamong infection with the two parasites. TNF containing cells, however, were clearly different between Sr-and Nb-infection. Percentage of TNF containing cells decreased in Sr-infection but not in Nb-infection.These results may be helpful to clarify the selective mechanism among different intestinal nematode species. We also examined mechanism of regulating mucus secretion in Nb-infected mice, because less IEL were recovered and more mucus seemed to be secreted in Nb-infection. We found that mucus secretion from goblet cells may be regulated by CD4^+ cells. Less

肠道线虫的驱虫、自然治愈机制是肠道线虫的直接效应细胞对寄生虫具有选择性。为了概括蠕虫驱逐的机制，我们需要了解调节直接效应细胞的机制。本研究以小肠上皮内细胞（IEL）为研究对象，采用流式细胞术和RT-PCR技术，对感染拉氏血吸虫（Sr）和巴西血吸虫（Nb）的小肠上皮内细胞（IEL）T细胞亚群和细胞因子的产生进行了研究，并对肠系膜淋巴结细胞进行了检测。Sr感染和Nb感染小鼠的IEL平均回收率分别小于正常小鼠IEL的40%和6%。Sr和Nb感染小鼠IEL中CD 3 ^+、CD 4 ^+、CD 8 ^+和γ δ TCR ^+细胞的百分率基本无差异。在Sr感染小鼠的IEL中检测到Th 2型细胞因子IL-4和IL-3 mRNA，而在正常IEL中未检测到Th 1型细胞因子IL-4和IL-3 mRNA， ...更多信息 IFN-γ mRNA在正常IEL中检测到，在感染中几乎没有变化。IEL中肥大细胞生长因子IL-3 mRNA表达增加，可能参与了Sr的排出。流式细胞仪检测肠系膜淋巴结细胞内细胞因子的表达。Sr和Nb感染组肠系膜淋巴结中IL-3阳性细胞数均增加。两种寄生虫感染后，IL-4和IFN-γ阳性细胞的数量无明显差异。然而，含TNF的细胞在Sr-和Nb-感染之间明显不同。Sr感染后，含TNF细胞的百分比下降，而Nb感染后，含TNF细胞的百分比没有下降。这些结果可能有助于阐明不同肠道线虫种之间的选择机制。我们还研究了Nb感染小鼠粘液分泌的调节机制，因为Nb感染小鼠的IEL较少，而粘液分泌似乎较多。我们发现杯状细胞的粘液分泌可能受CD 4 ^+细胞的调节。少