Development of multi-layered stem cell sheet transplant materialfor periodontal regenerative therapy

在本研究中，我们开发了一种多层干细胞片状移植材料，旨在建立一种新的牙周再生治疗方法。在常规培养液中，间充质干细胞在形成融合单层后停止增殖。然而，在转化生长因子-β-1和抗坏血酸的存在下，细胞继续生长并形成多层片状。在含有转化生长因子-β-1和抗坏血酸的培养液中加入Y-27632可阻止转化生长因子-β-1引起的细胞膜收缩。多层膜中的细胞表达与原始间充质干细胞相同的表面标志，并具有向多种细胞系分化的潜能。这些结果表明，这种多层干细胞片状移植材料可用于牙周再生治疗。

In this study, we developed a multi-layered stem cell sheet transplant material with the aim to establish a new method for periodontal regenerative therapy. In the conventional culture medium, the proliferation of the mesenchymal stem cells stopped after forming confluent monolayer. However, the cells continued to grow and formed multi-layer sheets in the presence of TGF-β1 and ascorbic acid. Further addition ofROCK inhibitor Y-27632 to the medium containing TGF-β1 and ascorbic acid prevented the contraction of the cell sheets caused by TGF-β1. The cells in the multi-layered sheetsexpressed the same surface markers with those of the original mesenchymal stem cells and had the potential to differentiate into many cell lineages. These results indicated that this multi-layered stem cell sheet transplant material is useful for periodontal regenerative therapy.