Method of Collection and Preservation of Urine Samples for Measurement of Urinary Volatile Organic Solvents as Indices of Biological Monitoring for Exposure to Organic Solvents

本研究的目的是开发一种用于测定尿液中高挥发性有机溶剂的尿液样本的采集和保存方法，因此，对于尿液样本的保存，发现充满尿液的样本瓶（样本瓶中没有顶部空间）的冷藏是非常可行的。另一方面，针对混合有机溶剂的暴露，建立了一种多组分标准溶液的同时简易制备方法，用于毛细管气相色谱法测定尿液中的有机溶剂。通过对有机溶剂使用车间的调查，研究了有机溶剂暴露浓度与2-HP-β-CD浓度之间的关系，并对2-HP-β-CD浓度与2-HP-β-CD浓度之间的关系进行了探讨。 ...更多信息 相关系数r和回归方程y=ax分别为甲苯[r=0.950，y=0.0270x]，甲基异丁基酮[r = 0.900，y =0.352x]，甲乙酮[r =0.919，y=0.519x]，异丙醇[r =0.940，y=1.095x]。根据有机溶剂暴露浓度与尿中有机溶剂浓度的回归直线方程，可计算出暴露至限值时的尿中有机溶剂浓度。未校正的观测值与有机溶剂暴露浓度的相关性比校正值更显著本研究建立了尿中挥发性有机溶剂测定的尿样采集和保存方法，有机溶剂暴露浓度与尿有机物浓度之间存在显著相关性。少

This study was for the purpose of developing an available method of collection and preservation of urine samples for measurement of urinary highly volatile organic solvents.Consequently, for preservation of urine samples, it was found out that the cold storage with a sample vial full of urine (there was no head space in the sample vial) was very available. And urine samples spiked with water-soluble beta-cyclodextrin and hydroxypropyl derivative (2-HP-beta-CD) was more available for preservation.On the other hand, for exposure to mixed organic solvents, the simultaneous simple preparation method of a multi-component standard solution was established for the measurement of urinary organic solvents using gaschromatography with capillary column. Namely, a simultaneous multi-component standard solution spiked with 2-HP-beta-CD was easily prepared with stability.From the investigation in the workshop using organic solvents, the relation between the concentration of organic solvent exposure … More and that of urinary organic solvent showed a significant correlation : correlation coefficient (r) and equation of regression line (y=ax) were toluene [r=0.950, y=0.0270x], MIBK [r=0.900, y=0.352x], MEK [r=0.919 y=0.519x], IPA [r=0.940, y=1.095x]. From the equation of the regression line between the concentration of organic solvent exposure and that of the urinary organic solvent, urinary organic solvent concentrations corresponding with exposure to threshold limit value could be calculated.In the case of concentration of urinary organic solvents, the observed value that was uncorrected showed more significant correlation with the concentration of organic solvent exposure than the corrected values (by creatinine or specific gravity).This study established the method of collection and preservation of urine samples for measurement of urinary volatile organic solvents, and showed significant correlation between the concentration of organic solvent exposure and that of urinary organic concentration. Less