Method of Collection and Preservation of Urine Samples for Measurement of Urinary Volatile Organic Solvents as Indices of Biological Monitoring for Exposure to Organic Solvents

本研究旨在为尿液中高挥发性有机溶剂的测定建立一种可行的尿样采集和保存方法。因此，对于尿样的保存，采用装满尿液的样品瓶(样品瓶中没有顶空)的冷藏是非常可行的。另一方面，对于混合有机溶剂暴露，建立了一种同时简易的多组分标准溶液的制备方法，用于毛细管色谱检测尿液中有机溶剂。也就是说，同时加入2-HP-β-CD的多组分标准溶液易于制备，具有稳定性。通过车间使用有机溶剂的考察，有机溶剂暴露浓度与…的关系相关系数(R)和回归方程(y=ax)分别为甲苯[r=0.950，y=0.0270x]、甲基异丁基酮[r=0.900，y=0.352x]、甲酮[r=0.919 y=0.519x]、异丙基甲苯[r=0.940，y=1.095x]。根据有机溶剂暴露浓度与尿液有机溶剂浓度之间的回归方程，可以计算出接触阈值对应的尿液有机溶剂浓度。以尿液有机溶剂浓度为例，未经校正的观测值与有机溶剂暴露浓度的相关性比校正后的值(肌酐或比重)更显著。本研究建立了尿液中挥发性有机溶剂浓度的采集和保存方法，并显示有机溶剂暴露浓度与尿有机浓度之间存在显著的相关性。较少

This study was for the purpose of developing an available method of collection and preservation of urine samples for measurement of urinary highly volatile organic solvents.Consequently, for preservation of urine samples, it was found out that the cold storage with a sample vial full of urine (there was no head space in the sample vial) was very available. And urine samples spiked with water-soluble beta-cyclodextrin and hydroxypropyl derivative (2-HP-beta-CD) was more available for preservation.On the other hand, for exposure to mixed organic solvents, the simultaneous simple preparation method of a multi-component standard solution was established for the measurement of urinary organic solvents using gaschromatography with capillary column. Namely, a simultaneous multi-component standard solution spiked with 2-HP-beta-CD was easily prepared with stability.From the investigation in the workshop using organic solvents, the relation between the concentration of organic solvent exposure … More and that of urinary organic solvent showed a significant correlation : correlation coefficient (r) and equation of regression line (y=ax) were toluene [r=0.950, y=0.0270x], MIBK [r=0.900, y=0.352x], MEK [r=0.919 y=0.519x], IPA [r=0.940, y=1.095x]. From the equation of the regression line between the concentration of organic solvent exposure and that of the urinary organic solvent, urinary organic solvent concentrations corresponding with exposure to threshold limit value could be calculated.In the case of concentration of urinary organic solvents, the observed value that was uncorrected showed more significant correlation with the concentration of organic solvent exposure than the corrected values (by creatinine or specific gravity).This study established the method of collection and preservation of urine samples for measurement of urinary volatile organic solvents, and showed significant correlation between the concentration of organic solvent exposure and that of urinary organic concentration. Less