Study on injury and remodeling the lung

肺损伤与重塑研究

• 批准号: 07670681
• 负责人: NAGAI Atsushi
• 金额: $ 1.47万
• 依托单位: Tokyo Women's Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670681/
• 关键词: Oxidant; elastase; apoptosis; necrosis; DNA; lung injury; 急性肺傷害; ネフローシス; レドックスサイクル; ナイアシン; ニコチナマイド; ATP; NAD

1)In vivo experiments(1)An Administration of niacin or nicotinamide inhibited oxidant-induced acute lung injury.(2)Elastase induced detachment of airway epithelium, and SLPI protected the cells from the detachment.(3)Neutrophil sequestration induced by G-CSF did not produce acute inflammation in the lung.(4)P-selectin antagonist inhibited acute lung inflammation induced by LPS.2)In vitro experiments(1)Oxidant induced necrosis and/or apoptosis in the airway epithelium, and these effects ware in dose-and time-dependent fashion.(2)TNF alpha induced apoptosis in the lung fibroblasts.(3)Depletion of cystine induced apoptosis in the lung fibroblasts and airway epithelium. These findings suggest that intra-celluar reactive oxygen may be associated with signal transduction of apoptosis.

1)体内实验(1)烟酸或烟酰胺对氧化性急性肺损伤有抑制作用。(2)弹性蛋白酶诱导气道上皮脱离，而SLPI具有保护作用。(3) G-CSF诱导的中性粒细胞隔离未引起肺急性炎症。(4) p -选择素拮抗剂抑制lps诱导的急性肺炎症。(2)体外实验(1)氧化诱导的气道上皮坏死和/或凋亡，这些作用呈剂量和时间依赖性。(2)TNF α诱导肺成纤维细胞凋亡。(3)胱氨酸缺失诱导肺成纤维细胞和气道上皮细胞凋亡。这些发现提示细胞内活性氧可能与细胞凋亡的信号转导有关。

项目成果

Ozawa Yuri: "Apoptosis and necrosis induced by H_2O_2 and TNFα in bovine bronchial epithelum" Am J Respir Crit Care Med. 151. A546- (1995)

Yuri Ozawa：“牛支气管上皮中 H_2O_2 和 TNFα 诱导的细胞凋亡和坏死”Am J Respir Crit Care Med 151. A546- (1995)

Yasui Shuji: "Efects of cigarette smoke on cultured human fetal lung fibroblasts" Am J Respir Crit Care Med. (in press). (1996)

Yasui Shuji：“香烟烟雾对培养的人胎肺成纤维细胞的影响”Am J Respir Crit Care Med。

M.Hayashi: "Detection of p53 mutations in pleural effusion in patients with lung cancer." Am J Respir Crit Care Med. 151. A357 (1995)

M.Hayashi：“肺癌患者胸腔积液中 p53 突变的检测。”

S.Yasui: "A specific neutrophil elastase inhibitor (ONO-5046・Na) attenuates bleomycin-induced acute lung inflammation in the hamster." Am J Respir Crit Care Med. 151. A62 (1995)

S. Yasui：“一种特定的中性粒细胞弹性蛋白酶抑制剂 (ONO-5046·Na) 可减轻博莱霉素引起的仓鼠急性肺部炎症。”Am J Respir Crit Care Med 151. A62 (1995)

A.Nagai: "Basal surface of bronchial epithelial cells susceptible to elastase exposure." The Lung. 3. 147-152 (1995)

A.Nagai：“支气管上皮细胞的基底表面对弹性蛋白酶暴露敏感。”