Molecularepidemiology of Parainfluenza virus type 4 infection

副流感病毒4型感染的分子流行病学

• 批准号: 07670684
• 负责人: KOMADA Hiroshi
• 金额: $ 1.34万
• 依托单位: Suzuka University of Medical Scinece and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670684/
• 关键词: parainfluenza virus type 4; expressed cell; HN protein; ELISA; virus neutralization; パラインフルエンザウイルス4型; F蛋白; HN蛋白; シークエンス

About 250 human sera were collected, and they were analyzed by enzyme linked-immunosorbent assay (ELISA). We found that about 20 sera were positive for human parainfluenza type 4 virus (HPIV-4). The same sera had neutralization activity against HPIV-4. However, it was impossible to distinguish whether the sera inhibited HPIV-4A or 4B growth. To analyze whether the infection was caused by HPIV-4A or 4B,we established L929 cell lines which express HN proteins of HPIV-4A or 4B,because there was a big antigenic difference between HPIV-4A and 4B HN proteins. The identification is now in progress.To elucidate the cause of the antigenic difference between HPIV-4A and 4B,3 mutated clones of 4B HN gene were constructed, and we established L929 cell lines which express the mutated HN proteins of HPIV-4B.The antigenicity was analyzed by flow cytometry using monoclonal antibodies against HPIV-4, and it was indicated that the sugar binding sites may in part contribute to the antigenic difference between HPIV-4A an 4B HN proteins.

收集约250份人血清，采用酶联免疫吸附试验（ELISA）进行分析。我们发现约20份血清对人类副流感病毒4型（HPIV-4）呈阳性反应。同样的血清对HPIV-4有中和活性。然而，无法区分血清是否抑制HPIV-4A或4B的生长。为了分析感染是由HPIV-4A还是4B引起，我们建立了表达HPIV-4A或4B的HN蛋白的L929细胞系，因为HPIV-4A和4B的HN蛋白存在较大的抗原差异。鉴定工作正在进行中。为了阐明HPIV-4A与4B抗原差异的原因，构建了4B HN基因的3个突变克隆，并建立了表达HPIV-4B突变HN蛋白的L929细胞系。用抗HPIV-4的单克隆抗体对其抗原性进行了流式细胞术分析，结果表明糖结合位点可能是HPIV-4A和4B HN蛋白抗原性差异的部分原因。

项目成果

伊藤守弘: "ヒト型パラインフルエンザ4B型ウイルスF蛋白の遺伝子解析および発現" 鈴鹿医療科学技術大学紀要. 2. 125-132 (1995)

伊藤守宏：“人副流感4B型病毒F蛋白的基因分析和表达”铃鹿医科大学通报2. 125-132（1995）。

Noriko watanabe, Hiroshi Komada, Yasuhiko Ito et al.: "Identification the sequence responsible for nuclear targeting of the V protein of human parainfuruenza virus type 2." Journal of General Virology. 77. 327-338 (1996)

Noriko watanabe、Hiroshi Komada、Yasuhiko Ito 等人：“鉴定了负责人副流感病毒 2 型 V 蛋白核靶向的序列。”

Shinji Ohgimoto: "Molecular Characterization of fusion regulatury protein-1 that induces multinacleated giant cell formation of monocytes and HIVqp160-mediated cell fusion" Journal of Immunology. 155. 3585-3592 (1995)

Shinji Ohgimoto：“诱导单核细胞多核巨细胞形成和 HIVqp160 介导的细胞融合的融合调节蛋白 1 的分子特征”《免疫学杂志》。

Shigeru. Suga: "Identification of fusion regulatory protein(FRP)-1/4F2 related molecules" Cell Structure and Function. 20. 473-483 (1995)

茂。

Noriko Watanabe: "Identification of the sequences responsible for nuclear targeting of the V protein of human parainfluenza virus type 2" Journal of General Virology. 77. 327-338 (1996)

Noriko Watanabe：“负责人类副流感病毒 2 型 V 蛋白核靶向的序列的鉴定”普通病毒学杂志。