Gene analysis of human parainfluenza virus type 4 and analysis of virus receptor

人副流感病毒4型基因分析及病毒受体分析

• 批准号: 13670304
• 负责人: KOMADA Hiroshi
• 金额: $ 2.3万
• 依托单位: Suzuka University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670304/
• 关键词: Parainfluenza virus type 4; gene analysis; lymphocyte; stimulation; CD98HC; IL-2; IL-2R; モノクローナル抗体; リンパ球増殖; パラインフルエンザウイルス4型; transgenicマウス; HPIV-4; Tgマウス; mitogenesis

We analyzed the gene sequence of human parainfluenza virus type 4 L protein. More than 80% was finished, and only gene ending region was remaining.CD98 is a widely expressed cell surface heterodimetric protein of 125kDa. Its expression is up-regulated during lymphocyte activation induced by mitogen, superantigen, conventional antigen, arid a combination of phorbol myristate acstate (PMA) and ionomycin. However, the role of CD98 in the immune system is not so well understood. The role of CD98 in lymphocyte proliferation was investigated, especially in correlation with IL-2/IL-2R system. Monoclonal antibody (mAb) (26-24) directed against murine CD98 heavy chain (mCD98HC) suppressed the proliferation of lymphocytes stimulated with concunavalin A (Con A). However, anti-CD98HC mAb (26-24) did not inhibit the production of interleukin 2 (IL-2) by lymphocyte stimulated with Coil A. In addition, IL-2 (recombinant), which induced considerable DNA synthesis of lymphocytes stimulated with sub-optimal dose of Con A, did not induce CD98HC expression in lymphocytes. Anti-mCD98HC mAb did not suress the expression of IL-2Rα. Anti-LL-2RαmAb, which suppressed DNA synthesis, did not inhibit the expression of CD98HC. Taken together with these findings, it was inferred that CD98 system is independent of IL-2-IL-2R system in lymphocyte activation.

我们分析了人副流感病毒4型L蛋白的基因序列。CD 98是一种广泛表达的细胞表面异源蛋白，分子量为125 kDa。在丝裂原、超抗原、常规抗原以及佛波酯和离子霉素联合诱导的淋巴细胞活化过程中，其表达上调。然而，CD 98在免疫系统中的作用还不太清楚。研究了CD 98在淋巴细胞增殖中的作用，特别是与IL-2/IL-2 R系统的关系。针对鼠CD 98重链（mCD 98 HC）的单克隆抗体（mAb）（26-24）可抑制伴刀豆球蛋白A（Con A）刺激的淋巴细胞增殖。然而，抗CD 98 HC mAb（26-24）不抑制Coil A刺激的淋巴细胞产生白细胞介素2（IL-2）。此外，IL-2（重组），诱导相当大的DNA合成的淋巴细胞刺激的次优剂量的刀豆蛋白A，不诱导CD 98 HC在淋巴细胞中的表达。抗mCD 98 HC mAb不抑制IL-2 R α的表达。抑制DNA合成的抗LL-2 R αmAb不抑制CD 98 HC的表达。结合上述结果，推测CD 98系统在淋巴细胞活化中不依赖于IL-2-IL-2 R系统。

项目成果

Morihiro Ito, Shin-ichiro Takebayashi, Katsuzumi Okumura, Takeshi Ohkubo, Machiko Nishio, Mitsuo Kawano, Hiroshi Komada, Yoshiro Ito, Masato Tusrudome: "Up-regulated expression of a novel gene in activated human peripheral blood mononuclear cells that is

Morihiro Ito、Shin-ichiro Takebayashi、Katsuzumi Okumura、Takeshi Ohkubo、Machiko Nishio、Mitsuo Kawano、Hiroshi Komada、Yoshiro Ito、Masato Tusrudome：“在激活的人外周血单核细胞中上调一种新基因的表达，该基因是

Machiko Nishio, Masato Tsurudome, Morihiro Ito, Mitsuo Kawano, Hiroshi Komada, Yasuhiko Ito: "High resistance of human parainfluenza type 2 virus protein-expressing cells to the antiviral and anti-cell proliferative"activities of alpha/beta interferons :

Machiko Nishio、Masato Tsurudome、Morihiro Ito、Mitsuo Kawano、Hiroshi Komada、Yasuhiko Ito：“人副流感 2 型病毒蛋白表达细胞对 α/β 干扰素的抗病毒和抗细胞增殖活性具有高抵抗力”：

Masato Tsurudome, Morihiro Ito, Machiko Nishio, Mitsuo Kawano, Hiroshi Komada, Yasuhiko Ito: "Hemagglutinin-neuraminidase-independent fusion activity of simian virus 5 fusion protein: difference in conformation between fusogenic and nonfusogenic F J)rotei

Masato Tsurudome、Morihiro Ito、Machiko Nishio、Mitsuo Kawano、Hiroshi Komada、Yasuhiko Ito：“猿猴病毒 5 融合蛋白的血凝素-神经氨酸酶独立融合活性：融合与非融合 F J)rotei 之间的构象差异

Mori K., Komada H., Ito Y.et al.: "The functional interactions between CD98 and CD147 in regulating virus-induced cell fusion and osteoclast formation"Med.Micro.Immunol.. in press. (2003)

Mori K.、Komada H.、Ito Y.等人：“CD98 和 CD147 在调节病毒诱导的细胞融合和破骨细胞形成中的功能相互作用”Med.Micro.Immunol.. 正在出版。

Kozuka Y., Komada H., Ito Y et al.: "Identification of amimo acids essential for the human parainfluenza type 2 virus V protein to lower the intracellular levels of STAT2"Virology. 317. 208-214 (2003)

Kozuka Y.、Komada H.、Ito Y 等人：“鉴定人副流感 2 型病毒 V 蛋白所必需的氨基酸，以降低 STAT2 的细胞内水平”病毒学。