A morphological study of the brain in the ataxic mutant rat.

共济失调突变大鼠大脑的形态学研究。

基本信息

批准号：
07670708
负责人：
TAKAGISHI Yoshiko
金额：
$ 1.54万
依托单位：
NAGOYA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670708/
关键词：
Crerebellum Endoplasmic reticulum Purkinje cells Calucium Ataxia Mutant Neuronal plasticity Rat

项目摘要

An ataxic mutant rat, dilute-opisthotonus (dop), was originally discovered in a breeding colony of Wister rats and shows a dilution of coat color and neurological disorders with convulsivelimb movements and opisthotonic seizures continuing until the death of animals at 3 weeks of age. The animals have been bred in our laboratory by intercrossing the heterozygous (dop/+) rats. The dilute-opisthotonus gene was mapped on chromosome 8 by PCR-amplified microsatellite markers, which showed a linkage of Apoc3, dop and Mylclv genes, suggesting that this mutation was homologous to dilute-lethalof the mouse.Electronmicroscopy and immunocytochemistry using anti-inositol 1,4,5-triphosphate (InsP3) receptor antibody revealed that smooth endoplasmic reticulum (SER) was missing in the dendritic spine of Purkinje cell in the dop cerebellum. Observation of developing Purkinje cells showed that SER was formed in the soma and dendrites but not in the spine of the Purkinje cell.SER is assumed to be an intracellular Ca store and it has been suggested that a Ca signaling process at the synaptic site has a key role in synaptic transmission. Thus, the dop rat is thought to have impaired synaptic regulation. Electronmicroscopy and Immunocytochemistry using antibody raised against GABA,transmitter released by Purkinje cell terminals, indicated that Purkinje cell axon terminals normally contacted the neurons in deep cerebellarnuclei.

最初在北鸟大鼠的繁殖菌落中发现了一种共同的突变大鼠稀释性稀释剂（DOP），并显示出稀释的外套颜色和神经系统疾病，并具有抽搐的运动和蛋白石癫痫发作，直到3周的动物死亡。这些动物是通过将杂合（DOP/+）大鼠划入的实验室中的。通过PCR放大的微卫星标记在8染色体上映射稀释的基因，该标志物显示了ApoC3，DOP和MyLCLV基因的链接，这表明该突变与稀释的小鼠同源于小鼠。利用小鼠。利用抗肌术和免疫细胞化学抗体1 inst3 insption instion（ins）。抗体表明，在小脑DOP小脑的Purkinje细胞的树突状脊柱中缺少光滑的内质网（SER）。观察到发育中的purkinje细胞表明，SER是在soma和dendrites中形成的，但在浦肯野细胞的脊柱中没有形成。SER被认为是细胞内Ca储存库，已经建议突触部位的Ca信号传导过程在突触传播中具有关键作用。因此，DOP大鼠被认为受损的突触调节。使用针对GABA的抗体，Purkinje细胞末端释放的发射机，电子显微镜和免疫细胞化学表明，Purkinje细胞轴突末端正常接触深脑北核中的神经元。